Applied Thin–Layer Chromatography – Best Practice and Avoidance of Mistakes
Home > Mathematics and Science Textbooks > Chemistry > Applied Thin–Layer Chromatography – Best Practice and Avoidance of Mistakes
12%
Applied Thin–Layer Chromatography – Best Practice and Avoidance of Mistakes

Applied Thin–Layer Chromatography – Best Practice and Avoidance of Mistakes


     0     
5
4
3
2
1



Out of Stock


Notify me when this book is in stock
X
About the Book

Thin layer chromatography (TLC) is a powerful, fast and inexpensive analytical method. It has proven its usefulness in pharmaceutical, food and environmental analysis. This new edition features a completely revised chapter on documentation, now including the use of digital cameras. Selected new sorbents and instruments are also introduced. Why has the prior edition been successful? All steps of the analytical procedure are clearly explained, starting with the choice of a suitable TLC technique and ending with data evaluation and documentation. Special emphasis is put on the proper choice of materials for TLC. Properties and functions of various materials and the TLC equipment are described, covering e. g. precoated layers, solvents and developing chambers, and including information on suppliers. Many practical hints for trouble shooting are given. All this is illustrated with numerous coloured figures. How to use TLC in compliance with GLP/GMP regulations is described in detail, including the required documentation. Therefore the reader can very easily compile his own standard operating procedures.

Table of Contents:
INTRODUCTION What is TLC? When is TLC used? Where is TLC used How is the result of a TLC represented? What kinds of reference substances are used in TLC? The literature on TLC PRECOATED LAYERS Why use precoated layers? How are precoated layers produced? What kinds of precoated layers are there? What are precoated layers used for? Criteria for choosing stationary phases in TLC Effect of the stationary phase when the solvents are identical Recommendations for the procurement and storage of precoated layers Problems in the nomenclature and classification of precoated layers BEFORE TLC DEVELOPMENT Handling of the precoated layers Prewashing Activation Conditioning Impregnation Application of the sample Positioning of the sample Drying before development SOLVENT, DEVELOPING CHAMBERS AND DEVELOPMENT Solvent TLC developing chambers Development of thin-layer chromatograms Drying after development EVALUATION WITHOUT DERIVATIZATION Visual direct evaluation Optical direct evaluation using special equipment Coupling methods for substance identification Documentation without or before derivatization DERIVATIZATION Thermochemical reaction Irradiation with high energy light Reaction with reagents Special types of derivatization Treatment of derivatized chromatograms EVALUATION AFTER DERIVATIZATION Visual evaluation Evaluation by TLC scanner Evaluation by video system Evaluation by flat-bed scanner DOCUMENTATION Description of a thin-layer chromatogram Documentation by reproduction, tracing or photocopying Photographic documentation Video documentation TLC scanner documentation Flat-bed scanner documentation GMP/GLP-CONFORMING TLC WORK Validation of TLC methods Use of qualified calibrated equipment GMP/GLP-conforming rawdata sheets Examples of GMP/GLP-conforming testing procedures LOADING EFFECTS Definite loading of a substance TLC of sensitive substances SPECIAL METHODS IN TLC AMD - Automated Multiple Development OPLC - Overpressured Layer Chromatography HPPLC - High Pressure Planar Liquid Chromatography TLC-FID/FTID - Combination of TLC with Flame Ionization Detector or Flame Thermoionic Ionization Detector RPC - Rotation Planar Chromatography Appendix Chromatography and art References Abbreviations used Acknowledgements Market review

About the Author :
After working for many years for Heumann Pharma (now part of Pfizer), where she was in particular responsible for the training and education of lab technicians, Elke Hahn-Deinstrop is now a freelance consultant for thin-layer chromatography. She is author of numerous papers and frequent lecturer.

Review :
"...an excellent practical guide to the utilization of this useful analytical procedure." (Journal of Hazardous Materials, June 25, 2007) "This impressive book thoroughly discusses a subject not usually covered thoroughly in the analytical chemistry literature." (CHOICE, June 2007)


Best Sellers


Product Details
  • ISBN-13: 9783527610259
  • Publisher: Wiley-VCH Verlag GmbH
  • Publisher Imprint: Wiley-VCH Verlag GmbH
  • Height: 242 mm
  • No of Pages: 330
  • Weight: 744 gr
  • ISBN-10: 3527610251
  • Publisher Date: 16 Jan 2007
  • Binding: Other digital
  • Language: English
  • Spine Width: 22 mm
  • Width: 175 mm


Similar Products

Add Photo
Add Photo

Customer Reviews

REVIEWS      0     
Click Here To Be The First to Review this Product
Applied Thin–Layer Chromatography – Best Practice and Avoidance of Mistakes
Wiley-VCH Verlag GmbH -
Applied Thin–Layer Chromatography – Best Practice and Avoidance of Mistakes
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

Applied Thin–Layer Chromatography – Best Practice and Avoidance of Mistakes

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Bookswagon (the "CRR Service").


    By submitting any content to Bookswagon, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Bookswagon (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Bookswagon a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Bookswagon may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Bookswagon's sole discretion. Bookswagon reserves the right to change, condense, withhold publication, remove or delete any content on Bookswagon's website that Bookswagon deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Bookswagon does not guarantee that you will have any recourse through Bookswagon to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Bookswagon reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Bookswagon, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Bookswagon, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept

    Fresh on the Shelf


    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!