Filling a gap in the literature for a hands-on guide focusing on everyday laboratory challenges, this English edition has been expanded and revised using the feedback received on the successful German precursor. Throughout the book, Professor Mascher draws on his 30 years of
experience and provides abundant practical advice, troubleshooting and other hints highlighted in boxes, as well as a broad selection of walkthrough case studies. Based on a course taught by the author, the first part of the book intuitively explains all steps of routine bioanalysis and explains how to set up a robust, inexpensive and effi cient method for a given substance. In the second part he includes 20 worked example cases that highlight common challenges and how to overcome them.
With its appendix containing tried-and-tested analytical methods for 100 clinically relevant substances from the author`s own laboratory,
complete with spectral and MS data as well as literature references and basic pharmacokinetic information, this is a life-long companion for
everyone working in clinical, pharmaceutical and biochemical analysis.
Comments to the German book:
"The book comes to life through its examples, showing not only what did work in the author's laboratory, but also what didn't."
ChemieReport
"Indispensable for novices, while even old hands will be able to expand their knowledge. A collection of analytical data for ca. 100 substances completes the book's offering, leaving almost nothing to be desired."
pharmind
Table of Contents:
Preface vii
Acknowledgements ix
1 Introduction 1
1.1 First Question: Determination of Ibuprofen in Plasma 2
1.2 Second Question: Determination of Tryptophan in Urine 7
1.3 Third Question: Determination of Paclitaxel in Tissue 10
2 Planning of Analyses 13
2.1 Introduction 13
2.2 Limit of Detection (LOD) and Determination (LLOQ) 14
2.3 Detectors 15
2.4 Structure of the Analyte 17
2.5 Solubility of an Analyte 20
2.6 Selection of the Detector 21
3 Sample Preparation 23
3.1 Dilution 23
3.2 Protein Precipitation, Overview 23
3.3 Extraction 25
4 HPLC Separation 35
4.1 HPLC Pumps 36
4.2 Degasser 38
4.3 Injector 38
4.4 HPLC Columns 39
5 Detection 45
5.1 Detection in the Pharmaceutical/Bioanalytical Area 46
5.2 Detection in the “Clinical Area” (Therapy Control/Compliance) 46
6 Chemical Derivatization for Detection Enhancement 49
7 Validation Concepts 53
7.1 Introduction 53
7.2 Realization of the FDA Guideline 55
8 Practical Hints Concerning Stability, Destruction and Degradation Products 59
9 Metabolites 61
10 Internal Standards 63
11 Case Studies with Intensive Discussion for Each Substance 65
11.1 Acetylcarnitine in Plasma 66
11.2 Acetylcysteine in Plasma 68
11.3 Acyclovir in Plasma and Urine 72
11.4 Caffeine in Plasma 74
11.5 Diazepam in Plasma 78
11.6 Diclofenac in Plasma 79
11.7 Dihydralazine in Plasma 82
11.8 Duramycin (Moli1901) in Plasma 85
11.9 Fluticasone Propionate in Plasma 89
11.10 Hydroxytriamterene Sulfate and Triamterene in Plasma and Urine 92
11.11 Ibuprofen in Plasma (also Enantiomeric Separation) 94
11.12 Minocycline in Plasma 96
11.13 Norfloxacine in Plasma and Urine 98
11.14 Paclitaxel in Plasma, Urine and Tissue 101
11.15 Paracetamol (Acetaminophen) in Plasma 104
11.16 Pimelic Acid in Plasma and Urine 106
11.17 8-Prenylnaringenin in Plasma and Different Types of Tissues 108
11.18 Silibinin in Plasma 110
11.19 Valnemulin in Plasma, Different Tissue Types and in Animal Feed 115
11.20 Vitamin B1 (Total Thiamine) in Plasma 118
Table 11.1 Recommended books 121
Table 11.2 Properties of substances 124
Appendix 135
Short Description of Determination for about 100 Substances 135
Substances listed in the Appendix 138
A Short Explanation of Tables Presented in the Appendix 139
About the Author :
Hermann Mascher is founder and CEO of the independent contract research organization "pharm-analyt" based in Baden (near Vienna), Austria. A chemist by training, he has been dealing with quantitative determination of drugs, metabolites, phyto-ingredients and endogenous substances in plasma, urine and tissue for more than 30 years. Professor Mascher is regularly being consulted for analytical questions
of drugs, food as well as botanical and ecological issues. He has published over 80 publications majorly in peer reviewed journals and received several awards in science and innovation for his work in the analysis of clinically important substances.
Review :
"The book is clearly written and comprehensive. A lot of practical advice and tips makes it helpful for any practically oriented clinical laboratory and it can be counted as a valuable source for everyone working with chromatographic techniques in a bioanalytical laboratory." (Analytical and Bioanalytical Chemistry, 13 November 2012)
“This book is more than a very successful and useful user guide, and is a valuable tool for the laboratorywork, not only for clinical analysts, but also for biochemists, pharmacists, etc. In addition to a compact targeted representation of the most important theoretical foundations for the planning and execution of (clinical) analysis (sample preparation, HPLC separation, usage of different modes, detection capabilities, derivatization techniques to the point of validation), the readers receive valuable information for their work, which are explained on the basis of practical examples [pages 1–64].” (ChemMedChem, 1 November 2012)
