Carrier-bound Immobilized Enzymes: Principles, Application and Design

Carrier-bound Immobilized Enzymes The first systematic overview of this key technique since the early 1990s, this authoritative reference is the only handbook available to include all recent developments. The author draws on his wide-ranging experience in both academia and industry to systematically cover all types of enzyme immobilization methods, including such novel techniques as site-specific immobilization, imprinting and immobilization in organic solvents. From the contents: Introduction to Immobilized Enzymes Adsorption-based Immobilization Covalent Enzyme Immobilization Enzyme Entrapment Enzyme Encapsulation Unconventional Enzyme Immobilization Throughout, a careful review of materials and techniques for the preparation of functional immobilized enzymes that benefits both developers and users of carrier-bound enzymes. Essential reading for biotechnologists, chemists and food scientists using enzymes in their daily work.

Table of Contents:
IMMOBILIZED ENZYMES: PAST, PRESENT AND PROSPECTS Introduction The Past Implications from the Past Prospective and Future Development ADSORPTION-BASED IMMOBILIZATION Introduction and Classification Principles of Adsorptive Enzyme Immobilization Requirements of the Carriers Factors which Dictate Enzyme Performance Preparation of Immobilized Enzymes by Adsorption COVALENT ENZYME IMMOBILIZATION Introduction Physical Nature of Carriers Chemical Nature of Carriers Amino Acid Residues for Covalent Binding of Enzymes Factors Influencing Enzyme Performance Preparation of Active Carriers ENZYME ENTRAPMENT Introduction and Definition Requirements of the Carriers Effect of the Entrapment Preparation of Entrapped Enzymes ENZYME ENCAPSULATION Introduction and Classification Effect of Encapsulation Preparation of Encapsulated Enzymes NON-CONVENTIONAL ENZYME IMMOBILIZATION Coating-based Immobilization Site-specific Immobilization Immobilization in Organic Solvents Imprinting Immobilization Modification-based Immobilization Post-immobilization Techniques

About the Author :
Linqiu Cao studied Organic Chemistry and Biotechnology at the Beijing University of Science and Technology (China). He was a visiting scientist at GBF (Gesellschaft fur Biotechnologische Forschung) in Braunschweig (Germany) and later moved to the University of Stuttgart (Germany) to obtain a PhD in Biotechnology. He then joined the group of Roger Sheldon at the Technical University in Delft (The Netherlands), where he pioneered a novel enzyme immobilisation technology with improved performance in biocatalytic transformations using cross-linked enzyme aggregates (CLEAs). In 2000, he joined the newly founded company Avantium in Amsterdam (The Netherlands) where he continued to develop novel enzyme crosslinking and immobilisation technology. Recently he moved to DMV in Veghel (The Netherlands).

Review :
"The first systematic overview of this key technique since the early 1990s, this authoritative reference is the only handbook available to include all recent developments. (...) A must for biotechnologists, biochemists and preparative chemists using enzymes in their daily work." Clinical Laboratory "...the volume provides excellent and comprehensive review on the important field of enzyme immobilization technology covering both the history and present state of immobilization procedures. Numerous tables and figures throughout the volume provide illustrative material to support the detailed information presented in the text, and make this volume an excellent resource for biotechnologists, biochemists, biochemical engineers and enzyme technologists." Carbohydrate Polymers "Es wird dazu beitragen, der rationalen Kombination der vorhandenen Prinzipien zur Enzymimmobilisierung die Zukunft zu ebnen." Nachrichten aus der Chemie