Introduction to Fluorescence Sensing
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Introduction to Fluorescence Sensing

Introduction to Fluorescence Sensing


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About the Book

Fluorescence is the most popular technique in chemical and biological sensing and this book provides systematic knowledge of basic principles in the design of fluorescence sensing and imaging techniques together with critical analysis of recent developments. Its ultimate sensitivity, high temporal and spatial resolution and versatility enables high resolution imaging within living cells. It develops rapidly in the directions of constructing new molecular recognition units, new fluorescence reporters and in improving sensitivity of response, up to the detection of single molecules. Its application areas range from the control of industrial processes to environmental monitoring and clinical diagnostics. Being a guide for students and young researchers, it also addresses professionals involved in basic and applied research. Making a strong link between education, research and product development, this book discusses prospects for future progress.

Table of Contents:
Chapter 1. Introduction   Chapter 2. Basic principles   2.1. Overview of strategies in molecular sensing 2.2. Labeled targets in fluorescence assays 2.3. Competitor displacement assays 2.4. Sandwich assays 2.5. Catalytic biosensors 2.6. Direct reagent-independent sensing Sensing and thinking: How to make the best sensor? Comparison of basic principles   Chapter 3. Theoretical aspects   3.1. Parameters that need to be optimized in every sensor 3.2. Determination of binding constants 3.3. Modeling the ligand binding isotherm 3.4. Kinetics of target binding 3.5. Formats for fluorescence detection Sensing and thinking: How to provide the quantitative measure of target binding?   Chapter 4. Fluorescence detection techniques   4.1. Fluorescence fundamentals 4.2. Intensity-based sensing 4.3. Anisotropy-based sensing and polarization assays 4.4. Lifetime-based fluorescence response 4.5. Excimer and exciplex formation 4.6. Förster resonance energy transfer (FRET) 4.7. Wavelength-shift sensing 4.8. Two-band wavelength-ratiometric sensing with a single dye Sensing and thinking: The optimal choice of fluorescence detection technique   Chapter 5. Molecular-size fluorescence emitters   5.1. Fluorophores and their characteristics 5.2. Organic dyes as labels and tags 5.3. Organic dyes as fluorescence reporters 5.4. Visible fluorescent proteins 5.5. Luminescent metal complexes 5.6. Few-atom clusters of noble metals Sensing and thinking: Which molecular reporter to choose for particular needs?   Chapter 6. Nanoscale fluorescence emitters   6.1. Introduction to light emitting nano-world 6.2. Dye-doped nanoparticles and dendrimers 6.3. Conjugated polymers 6.4. Fluorescent carbon nanostructures 6.5. Semiconductor quantum dots 6.6. Up-converting nanocrystals Sensing and thinking: Nanoscale emitters, what are the advantages?   Chapter 7. Fluorescent nanocomposites   7.1. Fluorescence enhancement and quenching in nanocomposites 7.2. Modulation of emission parameters in multi-fluorophore systems 7.3. Optical choice of FRET donors and acceptors 7.4. Wavelength referencing, multiplexing and multicolor coding 7.5. Combining fluorescence with magnetic, NMR enhancing and other functionalities Sensing and thinking: Achieving multitude of functions in designed nanocomposites   Chapter 8. Recognition units   8.1. Multivalency: the principle of molecular recognition 8.2. Recognition units built of small molecules 8.3. Antibodies and their recombinant fragments 8.4. Ligand-binding proteins and protein-based display scaffolds 8.5. Designed and randomly synthesized peptides 8.6. Nucleic acid aptamers 8.7. Peptide nucleic acids 8.8. Molecularly imprinted polymers Sensing and thinking: Selecting the tools for optimal target recognition   Chapter 9. Mechanisms of signal transduction   9.1. General principles of signal transduction 9.2. Basic signal transduction mechanisms: electron, charge and proton transfer 9.3. Signal transduction via excited-state energy transfer 9.4. Superenhancement and superquenching 9.5. Signal transduction via conformational changes 9.6. Signal transduction via association and aggregation phenomena 9.7. Smart sensing with logical operations Sensing and thinking: How to couple the recognition and reporting functionalities?   Chapter 10. Supramolecular structures and interfaces for sensing   10.1. Self-assembled supramolecular systems10.2. Building blocks for supramolecular sensors10.3. Conjugation, labeling and cross-linking. 10.4. Supporting and transducing surfaces. 10.5. Functional lipid and polymer bilayersSensing and thinking: Extending sensing possibilities with smart nano-ensembles   Chapter 11. Non-conventional generation and transformation of response   11.1. Chemiluminescence and electrochemiluminescence 11.2. Bioluminescence 11.3. Radioluminescence and Cherenkov effect 11.4. Two-photon excitation and stimulated emission 11.5. Direct optical generation of electrical response signal 11.6. Evanescent-wave fluorescence sensors 11.7. Plasmonic enhancement of luminescence emission Sensing and thinking: Eliminating light sources and detectors: what remains?   Chapter 12. The sensing devices   12.1. Instrumentation for fluorescence spectroscopy 12.2. Optical waveguides and optodes 12.3. Multi-analyte spotted microarrays 12.4. Suspension arrays and barcoding 12.5. Microfluidic devices. 12.6. Devices incorporating whole living cells Sensing and thinking: Optimizing convenience, sensitivity and precision for obtaining the proper sensor response   Chapter 13. Focusing on targets   13.1. Temperature, pressure and gas sensing 13.2. Probing the properties of condensed matter 13.3. Detection of small molecules and ions 13.4. Nucleic acid detection and sequence identification 13.5. Recognition of protein targets 13.6. Polysaccharides, glycolipids and glycoproteins 13.7. Detection of harmful microbes Sensing and thinking: Adaptation of sensor units for multi-scale and hierarchical range of targets   Chapter 14. Sensing inside the living cells   14.1. Modern fluorescence microscopy 14.2. Super-resolution microscopy 14.3. Sensing and imaging on a single molecule level 14.4. Site-specific intracellular labeling and genetic encoding 14.5. Advanced nanosensors inside the cells 14.6. Sensing within the cell membrane 14.7. Sensing different targets in cell interior Sensing and thinking: Intellectual and technical means for addressing the systems of great complexity   Chapter 15. Sensing the whole body and clinical diagnostics   15.1. Ex-vivo diagnostics 15.2. Sensing the whole body 15.3. Monitoring the cells inside the living body 15.4. Theranostics: combining targeting, imaging and therapy Sensing and thinking: The strategy of controlling by light of diagnostics and treatment   Chapter 16. Opening new horizons   16.1. Genomics, proteomics and other ‘omics’ 16.2. The sensors to any target and to immense number of targets 16.3. New level of clinical diagnostics 16.4. Advanced sensors in drug discovery 16.5. Towards sensors that reproduce human senses 16.6. Sensors promising to change the society Sensing and thinking: Where do we stand and where should we go?   Epilogue.   Appendix. Glossary of terms used in fluorescence sensing   Index  


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Product Details
  • ISBN-13: 9783319207797
  • Publisher: Springer International Publishing AG
  • Publisher Imprint: Springer International Publishing AG
  • Edition: Revised edition
  • Language: English
  • Returnable: Y
  • Width: 155 mm
  • ISBN-10: 3319207792
  • Publisher Date: 20 Oct 2015
  • Binding: Hardback
  • Height: 235 mm
  • No of Pages: 794
  • Weight: 15031 gr


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