Histological and Histochemical Methods
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Histological and Histochemical Methods: Theory and Practice

Histological and Histochemical Methods: Theory and Practice


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About the Book

Now in its fourth edition, Histological and Histochemical Methods has been expanded and updated with the latest techniques and developments within the field, whilst retaining the details of the classic techniques still in use. The relations of chemical structures and reactions to fixation, tissue processing, staining, enzyme location, immunohistochemistry and other procedures are explained in simple, descriptive terms. This book is recommended for lecturers and students in biological and biomedical disciplines using histological and histochemical techniques. Postgraduate researchers will find this to be the ideal laboratory companion, as will medical laboratory technologists preparing for their professional examinations. Reviews: "John Kiernan has published extensively on histochemical methods since his first scientific paper in 1964. His textbook encompasses his wide personal experience and interest in dyes, staining and histochemical methodologies...I personally believe that this text should be found on the bookshelves of every histology and histochemistry laboratory as a basic reference source." Raymond Coleman, Acta Histochemica, April 2010

Table of Contents:
1. Introduction to microtechnique 2. Fixation 3. Decalcification and other treatments for hard tissues 4. Processing and mounting 5. Dyes 6. Histological staining in one or two colours 7. Staining blood and other cell suspensions 8. Methods for connective tissue 9. Methods for nucleic acids 10. Organic functional groups and protein histochemistry 11. Carbohydrate histochemistry 12. Lipids 13. Methods for inorganic ions 14. Enzyme histochemistry: general considerations 15. Hydrolytic enzymes 16. Oxidoreductases 17. Methods for soluble organic compounds of low molecular weight 18. Metal reduction and precipitation methods 19. Immunohistochemistry 20. Miscellaneous data Bibliography Glossary Index

Review :
'This book would serve as an excellent introduction for undergraduate and PhD students and will easily guide researchers and technicians in the comprehension of new techniques. The author emphasizes the importance of acquiring laboratory skills under the guidance of more experienced colleagues, which of course is true, but this book provides a wonderful introduction to the 'new researcher' and provides alternatives to the 'more experienced colleague'. This book is a 'must have' for any laboratory using histological techniques. It is an excellent teaching resource for anyone new to the field and gives ideas to the more experienced histologist. This book is not for the bookshelf - it should live on your laboratory bench.' Journal of Anatomy'The book is written in a succinct manner and is very readable despite being a technical manual. Numerous diagrams on chemical structures and reactions with excellent legends also make this book very user-friendly.Besides providing the usual cookbook-type recipes for the various staining methods, the author also carefully explains the theory and biochemistry behind the methodology. This information, a valuable asset in troubleshooting, would be very helpful to students, histotechnologists, and even pathologists and researchers...the concise and well written text makes this book a very handy manual for all laboratories involved in histology and histochemistry.'Journal of Histotechnology


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Product Details
  • ISBN-13: 9781904842422
  • Publisher: Scion Publishing Ltd
  • Publisher Imprint: Scion Publishing Ltd
  • Edition: Revised edition
  • Language: English
  • Spine Width: 20 mm
  • Weight: 1205 gr
  • ISBN-10: 1904842429
  • Publisher Date: 01 Mar 2008
  • Binding: Paperback
  • Height: 234 mm
  • Returnable: N
  • Sub Title: Theory and Practice
  • Width: 156 mm


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