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Home > Science, Technology & Agriculture > Biochemical engineering > Biotechnology > Genetic engineering > Lambda Phage as a Model Organism: Regulatory-Proteins and the Life of a Lambda
Lambda Phage as a Model Organism: Regulatory-Proteins and the Life of a Lambda

Lambda Phage as a Model Organism: Regulatory-Proteins and the Life of a Lambda


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About the Book

Professor Ira Herskowitz, one of the world's leading molecular geneticists, introduces Lambda, a virus that infects bacteria. The programme details the laboratory procedures for observing the phage life cycle and by using advanced graphic effects, allows viewers to see the program of infection, which includes DNA replication and synthesis of phage heads and tails. This book is intended for undergraduates in introductory biology (after students have heard about the relationship between gene and protein) and introductory courses dealing with health issues, such as HIV; graduate students as a supplement to molecular biology; students that have been exposed to some molecular biology; upper level molecular genetics; microbial genetics; or microbiology laboratory.

Table of Contents:
Part 1 Viruses: what, why and how?; Lambda are the simplest organisms capable of reproduction and is completely safe for humans to work with; it is easy to grow and grow only in bacterium E coli; genome is small and well-understood and because the phage life cycle grows exponentially and fast, it makes it possible to find extremely rare mutants. Part 2 The life cycle of Lambda: a simple time-lapsed 60-minute procedure is shown, in which viewers observe the effects of Lambda growth in a flask; a second procedure is done showing the effects of Lambda growth on a petri dish. Part 3 Regulatory proteins in Lambda: N protein, a regulatory protein that turns on the "early genes," O and P is discussed; an experiment is conducted showing that the joining of phage heads and tails can occur in vitro, outside of a living cell. Part 4 The latent state: prophage activation is defined and demonstrated through laboratory procedures; an experiment is conducted showing that bacteria carrying Lambda DNA in a latent state cannot be killed by infecting Lambda. Part 5 Biomedical applications: the herpes simplex virus is discussed in terms of the procedures the viewer has just seen.


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Product Details
  • ISBN-13: 9781888902013
  • Publisher: Cogito Learning Media Inc
  • Publisher Imprint: Cogito Learning Media Inc
  • Language: English
  • Sub Title: Regulatory-Proteins and the Life of a Lambda
  • ISBN-10: 1888902019
  • Publisher Date: 15 Sep 1996
  • Binding: VHS video
  • Returnable: N
  • Weight: 304 gr


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