Molecular Methods for Microsporidia Detection
Home > Mathematics and Science Textbooks > Science: general issues > Scientific research and technological innovation > Molecular Methods for Microsporidia Detection: Use of an Inhibitor Control with Real-Time PCR(Water Research Foundation Report Series)
Molecular Methods for Microsporidia Detection: Use of an Inhibitor Control with Real-Time PCR(Water Research Foundation Report Series)

Molecular Methods for Microsporidia Detection: Use of an Inhibitor Control with Real-Time PCR(Water Research Foundation Report Series)

|
     0     
5
4
3
2
1




Out of Stock


Notify me when this book is in stock
About the Book

Detection of microorganisms in the U.S. water supply is crucial to the prevention of waterborne disease and bioterrorism. There is a current and critical need for optimized and standardized methods and evidence-based comparisons of methods to detect waterborne pathogens. Despite the advantages that molecular methods offer for improved sensitivity, specificity, and species confirmation, historical microscopic methods are considered to be the gold standard; molecular detection of microbes from source water is an ongoing area of research and no consensus methods currently exist. If molecular methods, such as polymerase chain reactions (PCR), are to supplement and/or replace microscopic methods, new methods must be verified and validated according to the highest standards of both sensitivity and specificity, so that false-negative and false-positive results may be avoided and evidence based risk-management decisions can be made.  The objective of this project was to optimize a robotic nucleic acid extraction method and design a real-time polymerase chain reaction (PCR) assay to detect microsporidian spores, which are currently listed on the U.S. Environmental Protection Agency’s Contaminant Candidate List (CCL). To accomplish this objective, the project team compared the efficiency of the nucleic acid extraction methods with those previously used for extraction of microsporidian spores in laboratory water and feces. Using laboratory water spiked with E. intestinalis spores, robotic nucleic acid extraction methods were compared by using numeric cycle threshold output values (Ct values), which are indirectly proportional to the target DNA concentration in a real-time PCR. The Ct values were used to guide decisions for optimizing the method’s performance under various experimental conditions. The method’s analytical sensitivity was determined in laboratory water. An additional synthetic oligonucleotide target was then added to serve as an IC. IC Cycle threshold values (IC-Ct) were used to monitor source water samples for the presence of PCR inhibitors, which could cause false negative results. Standardized protocols were documented and the PCR method was used to detect microsporidian spores spiked into various source water samples.


Best Sellers


Product Details
  • ISBN-13: 9781843398073
  • Publisher: IWA Publishing
  • Publisher Imprint: IWA Publishing
  • Height: 234 mm
  • No of Pages: 92
  • Spine Width: 18 mm
  • Width: 156 mm
  • ISBN-10: 1843398079
  • Publisher Date: 14 Oct 2008
  • Binding: Paperback
  • Language: English
  • Series Title: Water Research Foundation Report Series
  • Sub Title: Use of an Inhibitor Control with Real-Time PCR


Similar Products

Add Photo
Add Photo

Customer Reviews

REVIEWS      0     
Click Here To Be The First to Review this Product
Molecular Methods for Microsporidia Detection: Use of an Inhibitor Control with Real-Time PCR(Water Research Foundation Report Series)
IWA Publishing -
Molecular Methods for Microsporidia Detection: Use of an Inhibitor Control with Real-Time PCR(Water Research Foundation Report Series)
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

Molecular Methods for Microsporidia Detection: Use of an Inhibitor Control with Real-Time PCR(Water Research Foundation Report Series)

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Bookswagon (the "CRR Service").


    By submitting any content to Bookswagon, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Bookswagon (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Bookswagon a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Bookswagon may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Bookswagon's sole discretion. Bookswagon reserves the right to change, condense, withhold publication, remove or delete any content on Bookswagon's website that Bookswagon deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Bookswagon does not guarantee that you will have any recourse through Bookswagon to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Bookswagon reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Bookswagon, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Bookswagon, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept

    New Arrivals

    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!