Protocols for Semen Analysis in Clinical Diagnosis
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Protocols for Semen Analysis in Clinical Diagnosis

Protocols for Semen Analysis in Clinical Diagnosis


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About the Book

Once considered a passive variable in the fertility equation, sperm is now a directly observed, manipulated, potentially enhanced, and centrally determining factor in the overall fertility treatment regimen. Not only does contemporary fertility science place tremendous emphasis on sperm quality, tests such as semen analysis are relatively easier and more cost-effective for couples seeking fertility treatment than those performed on oocytes. Protocols for Semen Analysis in Clinical Diagnosis defines basic semen analysis concepts, provides clinically essential reference values, and elucidates the often subtle relationships between many sperm characteristics and their relevance for clinical diagnosis. The author takes you logically and systematically through each step of the diagnostic process. He discusses the underlying rationale for implementing different kinds of semen analysis, the intricacies of routine versus specialized testing, and additional diagnostic testing. With so many diverse semen analysis assays currently available, choosing the specific regimen appropriate for each patient can prove complex and confusing. Designed to supplement other more general, less clinically oriented texts, Protocols for Semen Analysis in Clinical Diagnosis provides easy to use procedural protocols for diagnosis.

Table of Contents:
Preface Introduction 1. Sperm Procurement Techniques 2. Semen Collection 3. Factors Affecting Ejaculate Counts 4. Biological Foundation for Specific Semen Assays 5. Questions and Answers 6. Semen Variable Designation 7. Semen Analysis and Sperm Assays 8. Can Sperm Get to the Fertilization Site? 9. Macroscopic Analysis 10. Microscopic Analysis 11. Sperm Mucus Penetration Test 12. Specialized Semen Analysis 13. Specialized Tests: When Routine Results are 'Normal' 14. Specialized Tests: When Routine Results are 'Abnormal' 15. Optional Clinical Semen Analysis 16. Sperm Nucleus Integrity Assay 17. Reactive Oxygen Species 18. Sperm Acrosin Assay 19. Creatine Phosphokinase Activity 20. Quality Control and Quality Assurance 21. The Need for Quality Control 22. External and Internal Quality Control 23. Reliability and Repeatability 24. Quality Control and Quality Assurance 25. Quality Assurance: The Bottom Line 26. Diagnostic Semen Analysis 27. Optimizing Fertility Treatment 28. Reporting Results 29. End Goal: Restoration of Natural Fertilization Potential 30. Post script 31. Suggested Additional Reading Appendices i. Sample Collection Instructions and Patient Questionnaire ii. Media and Protein Supplementation iii. Leukocyte Detection Methods iv. Diff-Quik Sperm Morphology Staining Method v. In vitro Sperm Capacitation Induction vi. In vitro Sperm Capacitation Enhancement vii. Quality Control for Chemical Analysis


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Product Details
  • ISBN-13: 9781842142059
  • Publisher: Taylor & Francis Ltd
  • Publisher Imprint: CRC Press
  • Language: English
  • No of Pages: 94
  • Weight: 599 gr
  • ISBN-10: 1842142054
  • Publisher Date: 17 Dec 2002
  • Binding: Hardback
  • No of Pages: 94
  • Returnable: N


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Protocols for Semen Analysis in Clinical Diagnosis
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Protocols for Semen Analysis in Clinical Diagnosis
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