Peroxynitrite Detection in Biological Media: Challenges and Advances(Volume 7 Detection Science Series)

Peroxynitrite is a powerful oxidiser which can damage a wide array of molecules within cells, including DNA and proteins, leading to apoptosis, inflammation or cancer. Peroxynitrite detection and quantification provides critical information in understanding its biological implications. Attempts to investigate the behavior of peroxynitrite in vivo and in vitro have been hampered by the difficulty in detecting this highly reactive oxygen species. This book presents the current state of the art in this research field with contributions from scientific leaders in the field. The chapters make clear the associated challenges and development for selective and sensitive detection of peroxynitrite. This book is a timely addition to the literature, as the first in the field, dedicated to detecting this molecule in vivo. It will be welcomed by the community particularly medicinal and analytical chemists, developers of sensors and probes and analytical equipment manufacturers.

Table of Contents:
Peroxynitrite, the basics; Quantifying peroxynitrite: bridging the gap between chemistry, biology and immunology; Methods of peroxynitrite synthesis in the context of the development and validation of peroxynitrite sensors; Peroxynitrite sensitive electrochemically active matrices; Electrochemical detection of peroxynitrite in biological solutions: challenges and perspectives; Real time monitoring of peroxynitrite by stimulation of macrophages with microelectrodes; Electrophoretic methods for separation of peroxynitrite and related compounds; Investigation of peroxynitrite-biomembrane interactions using biomimetic interfaces; Recent approaches to enhance selectivity of peroxynitrite detection; Development of fluorescent probes for the detection of peroxynitrite; Reversible near infrared probes for peroxynitrite monitoring;

About the Author :
Serban Peteu is serving since 2011 as a Professor, adjunct in Chemical Engineering & Materials Science at Michigan State University, Michigan, United States and is also a Project manager and Principal investigator at the National Institute for Chemistry (ICECHIM) Bucharest Romania. He earned a PhD in Biotechnology and Bioengineering, with MS in Chemical Engineering, both from the “Politehnica” University Bucharest. His main research interests are in chemical sensors where he has advanced detection methods including for biological fluids. Both electrochemical and optochemical sensing have been investigated, with emphasis to response amplification via hybrid nano-architectures. Present works include catalytic interfaces for peroxynitrite quantification. He co-authored over 50 research publications: articles, book chapters, international patents and an RSC book co-edited in 2013. Sabine Szunerits is since 2009 Professor in Chemistry at the University Lille 1 and attached to the Interdisciplinary Research Institute (IRI). She holds a PhD in organic electrochemistry from the Queen Mary and Westfield College, London UK and a MA in Social Science from the Open University of London. She has become a member of the “Institut Universitaire de France” (IUF) in 2010. Her current research interests are in the area of material science with emphasis on the development of novel analytical platforms for the study of affinity binding events and in the modification of nanostructures for biomedical applications. She is co-author of more than 160 research publications, wrote several book chapters and has 6 patents.