Regulating with RNA in Bacteria and Archaea: (ASM Books)

Revealing the many roles of RNA in regulating gene expression For decades after the discoveries of messenger RNA, transfer RNA, and ribosomal RNA, it was largely assumed that the role of RNA in the cell was limited to shuttling the genomic message, chaperoning amino acids, and toiling in the ribosomes. Eventually, hints that RNA molecules might have regulatory roles began to appear. With the advent of genomics and bioinformatics, it became evident that numerous other RNA forms exist and have specific functions, including small RNAs (sRNA), RNA thermometers, and riboswitches to regulate core metabolic pathways, bacterial pathogenesis, iron homeostasis, quorum sensing, and biofilm formation. All of these functions, and more, are presented in Regulating with RNA in Bacteria and Archaea, written by RNA biologists from around the globe. Divided into eight sections-RNases and Helicases, Cis-Acting RNAs, Cis Encoded Base Pairing RNAs, Trans-Encoded Base Pairing RNAs, Protein Titration and Scaffolding, General Considerations, Emerging Topics, and Resources-this book serves as an excellent resource for established RNA biologists and for the many scientists who are studying regulated cellular systems. It is no longer a fair assumption that gene expression regulation is the provenance of proteins only or that control is exerted primarily at the level of transcription. This book makes clear that regulatory RNAs are key partners along with proteins in controlling the complex interactions and pathways found within prokaryotes.

Table of Contents:
Contributors Foreword Preface Acknowledgments About the Editors Section I: RNases and Helicases 1. RNase E and the High-Fidelity Orchestration of RNA Metabolism Katarzyna J. Bandyra and Ben F. Luisi 2. Enzymes Involved in Posttranscriptional RNA Metabolism in Gram-Negative Bacteria Bijoy K. Mohanty and Sidney R. Kushner 3. RNases and Helicases in Gram-Positive Bacteria Sylvain Durand and Ciarán Condon Section II: Cis-acting RNAs 4. RNA Thermometers in Bacterial Pathogens Edmund Loh, Francesco Righetti, Hannes Eichner, Christian Twittenhoff, Franz Narberhaus 5. Small Molecule-Binding Riboswitches Thea S. Lotz and Beatrix Suess 6. The T-Box Riboswitch: tRNA as an Effector to Modulate Gene Regulation Kiel D. Kreuzer and Tina M. Henkin 7. rRNA Mimicry in RNA Regulation of Gene Expression Michelle M. Meyer 8. Processive Antitermination Jonathan R. Goodson and Wade C. Winkler 9. Genes within genes in bacterial genomes Sezen Meydan, Nora Vázquez-Laslop, and Alexander S. Mankin 10. Leaderless mRNAs in the Spotlight: Ancient but Not Outdated! Heather J. Beck and Isabella Moll Section III: Cis-encoded base pairing RNAs 11. Type I Toxin-Antitoxin Systems: Regulating Toxin Expression via Shine-Dalgarno Sequence Sequestration and Small RNA Binding Sara Masachis and Fabien Darfeuille 12. Widespread Antisense Transcription in Prokaryotes Jens Georg and Wolfgang R. Hess Section IV: Trans-encoded base pairing RNAs 13. Small Regulatory RNAs in the Enterobacterial Response to Envelope Damage and Oxidative Stress Kathrin S. Fröhlich and Susan Gottesman 14. Carbohydrate Utilization in Bacteria: Making the Most Out of Sugars with the Help of Small Regulatory RNAs Svetlana Durica-Mitic, Yvonne Göpel, Boris Görke 15. Small RNAs Involved in Regulation of Nitrogen Metabolism Daniela Prasse and Ruth A. Schmitz 16. Bacterial Iron Homeostasis Regulation by sRNAs Sylvia Chareyre and Pierre Mandin 17. Small-RNA-Based Regulation of Bacterial Quorum Sensing and Biofilm Formation Sine Lo Svenningsen 18. Regulatory RNAs in Virulence and Host-Microbe Interactions Alexander J. Westermann Section V: Protein titration and scaffolding 19. Global Regulation by CsrA and Its sRNA Antagonists Tony Romeo and Paul Babitzke 20. 6S RNA, a Global Regulator of Transcription Karen M. Wassarman 21. Bacterial Y RNAs: Gates, Tethers, and tRNA Mimics Soyeong Sim and Sandra L. Wolin Section VI: General considerations 22. Proteins That Chaperone RNA Regulation Sarah A. Woodson, Subrata Panja, and Andrew Santiago-Frangos 23. Epitranscriptomics: RNA Modifications in Bacteria and Archaea Katharina Höfer and Andres Jäschke 24. RNA Localization in Bacteria Jingyi Fei and Cynthia M. Sharma 25. Sponges and Predators in the Small RNA World Nara Figueroa‐Bossi and Lionello Bossi 26. Bacterial Small RNAs in Mixed Regulatory Networks Anaïs Brosse and Maude Guillier 27. Dual-Function RNAs Medha Raina, Alisa King, Colleen Bianco, and Carin K. Vanderpool 28. Origin, Evolution, and Loss of Bacterial Small RNAs H. Auguste Dutcher and Rahul Raghavan Section VII: Emerging topics 29. Cross-regulation between bacteria and phages at a posttranscriptional Level Shoshy Altuvia, Gisela Storz, Kai Papenfort 30. Large Noncoding RNAs in Bacteria Kimberly A. Harris and Ronald R. Breaker 31. Synthetic Biology of Small RNAs and Riboswitches Jordan K. Villa, Yichi Su, Lydia M. Contreras, Ming C. Hammond Section VIII: Resources 32. Functional Transcriptomics for Bacterial Gene Detectives Blanca Perez-Sepulveda and Jay C.D. Hinton 33. Structure and Interaction Prediction in Prokaryotic RNA Biology Patrick R. Wright, Martin Mann, Rolf Backofen Index

About the Author :
Gisela Storz is an NIH Distinguished Investigator in the Eunice Kennedy Shriver National Institute of Child Health and Human Development in Bethesda, Maryland. She carried out graduate work with Dr. Bruce Ames at the University of California, Berkeley and postdoctoral work with Dr. Sankar Adhya at the National Cancer Institute and Dr. Fred Ausubel at Harvard Medical School. As a result of the serendipitous discovery of the peroxide-induced OxyS RNA in E. coli, one of the first small, regulatory RNAs to be found, much of the work in her lab has focused on the genome-wide identification of small RNAs and their characterization. Kai Papenfort is a Professor of Microbiology at the Ludwig Maximilians University of Munich, Germany. He received a diploma in biology from the University of Marburg and carried out graduate work with Dr. Jörg Vogel at the Max Planck Institute for Infection Biology and the Humboldt University of Berlin. In his postdoctoral work at the University of Würzburg and Princeton University, Dr. Papenfort studied the regulatory functions of small RNA in bacterial pathogens and their involvement in bacterial communication processes such as quorum sensing. His laboratory focuses on the molecular mechanisms underlying the regulation by small RNAs in the major human pathogen, Vibrio cholerae.

Review :
Finally! One spectacular volume provides us all we need to educate ourselves in the fascinating world of regulatory RNAs in bacteria and archaea. From the genetics to the biochemistry to the mechanisms to the structures to the physiology to the networks to the challenges for the future—it's all here—laid out for us in exceptional form by the world's key experts. This volume is an eye-opening must read about one of microbiology's most exciting emerging fields. -Bonnie L. Bassler, Ph.D., HHMI Investigator, Squibb Professor and Chair of the Department of Molecular Biology, Princeton University