Neuroprotection Methods and Protocols
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Neuroprotection Methods and Protocols: (399 Methods in Molecular Biology)

Neuroprotection Methods and Protocols: (399 Methods in Molecular Biology)


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About the Book

Neuroprotection is a topic of great importance in current neuroscience, both basic and clinical. The incidence of age-related neurodegenerative diseases could be expected to rise dramatically in the future owing to an aging population. Consequently, finding the means of retarding or preventing the progression of such diseases becomes increasingly important. This book focuses on basic perspective on neuroprotective approaches and scientists well recognized for their work have contributed chapters to this volume. Although findings on neuroprotection in the different pathologies become more and more frequent and detailed, it can be difficult for researchers to orient themselves in such a complicate field. For this reason, this book describes basic science discovery and the application of such research within different laboratories leading to the development of neuroprotective protocols. The main aim of this volume is thus to give an overview of methods used to study neuronal death and neuroprotection and to offer a really comprehensive step-by-step method in order to make clear not just the procedure but also the principles behind the use of it. At this purpose, the “Notes” section of each chapter represents a useful tool to solve technical problems and to help in reproducing the described methods.

Table of Contents:
Neuronal Death and Neuroprotection: A Review.- The Use of Propidium Iodide to Assess Excitotoxic Neuronal Death in Primary Mixed Cortical Cultures.- A Modified Silver Technique (de Olmos Stain) for Assessment of Neuronal and Axonal Degeneration.- Model of Acute Injury to Study Neuroprotection.- Organotypic Entorhino-Hippocampal Slice Cultures—A Tool to Study the Molecular and Cellular Regulation of Axonal Regeneration and Collateral Sprouting In Vitro.- Role of Nrf2-Dependent ARE-Driven Antioxidant Pathway in Neuroprotection.- Biochemical Methods to Assess the Coupling of Brain Energy Metabolism in Control and Disease States.- A Method for Isolating Prosurvival Targets of NF-?B/Rel Transcription Factors.- Functional Cloning of Genes Regulating Apoptosis in Neuronal Cells.- Characterization of mRNA Expression in Single Neurons.- Brain on a Chip: A Method to Detect Novel Neuroprotective Candidate Targets.- Intracerebral Infusion of Neurotrophic Factors.- Synthesis of Cell-Penetrating Peptides and Their Application in Neurobiology.- The Stem Cells as a Potential Treatment for Neurodegeneration.- In Vivo Tomographic Imaging Studies of Neurodegeneration and Neuroprotection: A Review.

Review :
From the reviews: "Research in neuroprotection is essential for the development of therapies for stroke and neurodegenerative diseases. This book reviews research methods currently used for prevention and slowing of central nervous system degenerative disorders. … Neurologists, neurosurgeons, and neuroscientists interested in research in neuroprotection are the main audience for this book. This is a comprehensive approach to research in neuroprotection. … the book is well written and structured with no drawbacks. ... a good book for neuroscience and neurological libraries." (Celso Agner, Doody’s Review Service, December, 2007)


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Product Details
  • ISBN-13: 9781617377075
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Height: 229 mm
  • No of Pages: 239
  • Series Title: 399 Methods in Molecular Biology
  • ISBN-10: 1617377074
  • Publisher Date: 25 Dec 2011
  • Binding: Paperback
  • Language: English
  • Returnable: Y
  • Width: 152 mm


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Neuroprotection Methods and Protocols: (399 Methods in Molecular Biology)
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