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Home > Mathematics and Science Textbooks > Biology, life sciences > Biochemistry > YAC Protocols: (349 Methods in Molecular Biology)
YAC Protocols: (349 Methods in Molecular Biology)

YAC Protocols: (349 Methods in Molecular Biology)


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About the Book

This completely revised and updated edition of YAC Protocols takes advantage of the many new advances in biology since the publication of the first edition, including the sequencing of the human genome, the genomes of a wide variety of other organisms, and the increased use of transgenic animals for understanding the molecular basis of human and animal disease. This volume also provides a much-needed update on the new techniques currently employed and to help redefine and illustrate the important roles still to be played by yeast artificial chromosome (YAC) technologies in the postgenomic age. YAC Protocols, Second Edition, demonstrates the enormous potential use of YAC technologies in understanding the role of genetics in maintaining health, promoting pathogenesis, and conferring susceptibility to disease. Offering solutions to the many problems that have plagued researchers, this new edition allows for the wider and more efficient use of YAC technologies for the analysis of genetic and pathogenic causes of human disease.

Table of Contents:
Markers, Selection, and Media in Yeast Artificial Chromosome Cloning.- Construction of Yeast Artificial Chromosome Libraries From Pathogens and Nonmodel Organisms.- Analysis and Screening of Yeast Artificial Chromosome Libraries by Filter Hybridization.- Analysis of Yeast Artificial Chromosome DNA by Restriction Digestion, Southern Blotting Nucleic Acid Hybridization, and Polymerase Chain Reaction.- Postgenomic Bioinformatic Analysis of Yeast Artificial Chromosome Sequence.- Transformation of Yeast Using Bioactive Beads With Surface-Immobilized Yeast Artificial Chromosomes.- Modification and Amplification of Yeast Artificial Chromosomes.- Modifying Yeast Artificial Chromosomes to Generate Cre/LoxP and FLP/FRT Site-Specific Deletions and Inversions.- Selective Isolation of Large Chromosomal Regions by Transformation-Associated Recombination Cloning for Structural and Functional Analysis of Mammalian Genomes.- A Polymerase Chain Reaction-Mediated Yeast Artificial Chromosome-Splitting Technology for Generating Targeted Yeast Artificial Chromosomes Subclones.- Genomic Reconstruction by Serial Mitotic Recombination of Yeast Artificial Chromosomes.- Targeting Vector Construction by Yeast Artificial Chromosome Modification.- Production of Yeast Artificial Chromosome Transgenic Mice by Pronuclear Injection of One-Cell Embryos.- Generation of Yeast Artificial Chromosome Transgenic Mice by Intracytoplasmic Sperm Injection.- Establishment of Cell Lines That Exhibit Correct Ontogenic Stage-Specific Gene Expression Profiles From Tissues of Yeast Artificial Chromosome Transgenic Mice Using Chemically Induced Growth Signals.- Application of Yeast Artificial Chromosomes in Fluorescence In Situ Hybridization.- Contribution of Yeast Artificial Chromosome-Based Physical Maps to the FinalAssembly of the Trypanosoma cruzi Genome.


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Product Details
  • ISBN-13: 9781617376733
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Edition: Revised edition
  • Language: English
  • Returnable: N
  • Width: 152 mm
  • ISBN-10: 1617376736
  • Publisher Date: 10 Dec 2010
  • Binding: Paperback
  • Height: 229 mm
  • No of Pages: 204
  • Series Title: 349 Methods in Molecular Biology


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