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Home > Mathematics and Science Textbooks > Biology, life sciences > Biochemistry > Glycobiology Protocols: (347 Methods in Molecular Biology)
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Glycobiology Protocols: (347 Methods in Molecular Biology)

Glycobiology Protocols: (347 Methods in Molecular Biology)


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About the Book

A distinguished panel of international experts reviews the importance of glycobiology, an interdisciplinary field encompassing chemistry, biochemistry, biology, physiology, and pathology. Glycobiology Protocols highlights important methodological progress that has been made in the field of glycobiology, helping scientists to answer specific questions on glycoprotein structures, on the biosynthesis of glycoconjugates, and on the functions of lipid- or protein-bound carbohydrates. Glycobiology Protocols contains specific methods for the analysis of the structures or functions of glycoconjugates, as well as of glycosyltransferases and glycosidases involved in the biosynthesis of glycans. The methods described for specific systems can be modified for investigations of similar biomolecules and tissues or cell types. Comprehensive and highly practical, Glycobiology Protocols provides researchers with a solid basis for studying the many aspects of glycobiology with state-of-the-art methods. The protocols follow the successful Methods in Molecular Biologya series format, with each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Table of Contents:
Application of Fluorophore-Assisted Carbohydrate Electrophoresis for the Study of the Dolichol Pyrophosphate-Linked Oligosaccharides Pathway in Cell Cultures and Animal Tissues.- Partial Purification of Mannosylphosphorylundecaprenol Synthase From Micrococcus luteus.- Assay of Dolichyl-Phospho-Mannose Synthase Reconstituted in a Lipid Matrix.- O-Mannosylation in Mammalian Cells.- Methods for Analysis of Unusual Forms of O-Glycosylation.- Structural Analysis of Naturally Occurring Sialic Acids.- Nonradioactive Trans-Sialidase Screening Assay.- Structural Determination of O-Glycans by Tandem Mass Spectrometry.- Detailed Structural Analysis of N-Glycans Released From Glycoproteins in SDS-PAGE Gel Bands Using HPLC Combined With Exoglycosidase Array Digestions.- Molecular Modeling of Glycosyltransferases.- ?-Galactoside ?2,6-Sialyltransferase and the Sialyl ?2,6-Galactosyl-Linkage in Tissues and Cell Lines.- Visualizing Intracellular Distribution and Activity of Core2 ?(1,6)N-Acetylglucosaminyltransferase-I in Living Cells.- Gene Expression Analysis of Glycosylation-Related Genes by Real-Time Polymerase Chain Reaction.- Analysis of the Glycodynamics of Primary Osteoblasts and Bone Cancer Cells.- Micromethods for the Characterization of Lipid A-Core and O-Antigen Lipopolysaccharide.- Assay for a Galactosyltransferase Involved in the Assembly of the O7-Antigen Repeat Unit of Escherichia coli.- High-Throughput Quantitation of Metabolically Labeled Anionic Glycoconjugates by Scintillation Proximity Assay Utilizing Binding to Cationic Dyes.- Quantitative Analysis of Mucins in Mucosal Secretions Using Indirect Enzyme-Linked Immunosorbent Assay.- Molecular Dissection of the Mouse Zona Pellucida.- Soluble Adamantyl Glycosphingolipid Analogs as Probes of GlycosphingolipidFunction.- Diagnosis of Krabbe Disease by Use of a Natural Substrate.- In Vitro Assays of the Functions of Calnexin and Calreticulin, Lectin Chaperones of the Endoplasmic Reticulum.- Quantitative Measurement of Selectin-Ligand Interactions.- Binding and Inhibition Assays for Siglecs.

Review :
"...a very valuable handbook for a wide audience of specialists involved in studies of carbohydrates and carbohydrate-containing biopolymers; glycobiologists, biochemists, bioorganic chemists, and molecular and cell biologists." -Biochemistry


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Product Details
  • ISBN-13: 9781617376382
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Height: 229 mm
  • No of Pages: 382
  • Series Title: 347 Methods in Molecular Biology
  • ISBN-10: 1617376388
  • Publisher Date: 19 Nov 2010
  • Binding: Paperback
  • Language: English
  • Returnable: Y
  • Width: 152 mm


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