Membrane Protein Protocols
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Home > Mathematics and Science Textbooks > Biology, life sciences > Biochemistry > Membrane Protein Protocols: Expression, Purification, and Characterization(228 Methods in Molecular Biology)
Membrane Protein Protocols: Expression, Purification, and Characterization(228 Methods in Molecular Biology)

Membrane Protein Protocols: Expression, Purification, and Characterization(228 Methods in Molecular Biology)


     0     
5
4
3
2
1



International Edition


X
About the Book

A collection of key techniques for the study of receptors and transport proteins. The book provides examples of how different membrane proteins can be overexpressed in both prokaryotic and eukaryotic expression systems, how natural and overexpressed proteins can be solubilized from their host membranes, and how the solubilized protein can be purified in active form. Each protocols contains step-by-step instructions to ensure success, troubleshooting advice, lists of reagents, and tips on avoiding pitfalls.

Table of Contents:
Expression of Membrane Proteins.- Expression and Purification of the Amphipathic Form of Rabbit Cytochrome b5 in Escherichia coli.- Dihydroorotate Dehydrogenase of Escherichia coli.- A General Approach for Heterologous Membrane Protein Expression in Escherichia coli.- Expression of Membrane-Bound Iron-Sulfur Proteins.- Heterologous Expression of Human Scavenger Receptor Class B Type I (SR-BI) in Pichia pastoris.- Expression of Oligomeric Amiloride-Sensitive Epithelial Sodium Channel in Sf9 Insect Cells.- Functional Expression of His-Tagged Rhodopsin in Sf9 Insect Cells.- Detergent Selection in Membrane Protein Purification.- Preparation of Glycerol Facilitator for Protein Structure and Folding Studies in Solution.- Solubilization of Chemokine Receptors from Cell Membranes.- A Systematic Approach for the Solubilization of the Integral Membrane Protein Lysophospholipid.- Membrane Protein Purification.- Purification of Omp50, a Minor Porin of Campylobacter jejuni.- Purification of Porins from Mycobacterium smegmatis.- Isolation of the Melanocortin 5 Receptor.- Purification of Mammalian Serine Palmitoyltransferase, a Hetero-Subunit Enzyme for Sphingolipid Biosynthesis, by Affinity-Peptide Chromatography.- Purification of Phosphatidylglycerophosphate Synthase from Cultured Mammalian Cells.- Purification of Pancreastatin Receptor from Rat Liver Membranes.- Purification, Reconstitution, and Functional Characterization of Zinc Transporter from Rat Renal Brush Border Membranes.- Isolation of Lipid Raft-Associated Proteolipids.- Purification of Membrane-Bound Catechol-O-Methyltransferase.- Purification and Characterization of Transporter Proteins from Human Erythrocyte Membrane.- Purification of the Human Erythrocyte PS-Stimulated Mg2+-ATPase.- Reconstitution and Assay of Biogenic Membrane-Derived Phospholipid Flippase Activity in Proteoliposomes.- Expression, Purification, and Reconstitution of Rat Liver Carnitine Palmitoyltransferase I.- Structural Analysis of Membrane Proteins.- Crystallization in Lipidic Cubic Phases.- Optical Biosensor Assay Using Retroviral Receptor Pseudotypes.

Review :
"Investigators working with membrane proteins will want to acquire this book. This is a useful compilation of protocols. . .there is much of utility in this book"-Doody's Health Sciences Book Review Journal "...a useful reference work for researchers investigating the structure and function of membrane proteins." -- Immunology News "...provides good food for thought for new and advanced membrane protein researchers." - ChemBioChem "...highly recommendable to people working in proteomics..." - Proteomics Journal


Best Sellers


Product Details
  • ISBN-13: 9781617373763
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Height: 229 mm
  • No of Pages: 334
  • Series Title: 228 Methods in Molecular Biology
  • Width: 152 mm
  • ISBN-10: 1617373761
  • Publisher Date: 10 Nov 2010
  • Binding: Paperback
  • Language: English
  • Returnable: Y
  • Sub Title: Expression, Purification, and Characterization


Similar Products

Add Photo
Add Photo

Customer Reviews

REVIEWS      0     
Click Here To Be The First to Review this Product
Membrane Protein Protocols: Expression, Purification, and Characterization(228 Methods in Molecular Biology)
Humana Press Inc. -
Membrane Protein Protocols: Expression, Purification, and Characterization(228 Methods in Molecular Biology)
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

Membrane Protein Protocols: Expression, Purification, and Characterization(228 Methods in Molecular Biology)

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Bookswagon (the "CRR Service").


    By submitting any content to Bookswagon, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Bookswagon (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Bookswagon a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Bookswagon may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Bookswagon's sole discretion. Bookswagon reserves the right to change, condense, withhold publication, remove or delete any content on Bookswagon's website that Bookswagon deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Bookswagon does not guarantee that you will have any recourse through Bookswagon to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Bookswagon reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Bookswagon, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Bookswagon, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept

    Fresh on the Shelf


    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!