Protein'Protein Interactions: Methods and Applications(261 Methods in Molecular Biology)

A collection of highly successful biochemical, biophysical, genetic, and computational techniques for studying protein-protein interactions. These readily reproducible methods demonstrate how to identify protein interaction partners, qualitatively or quantitatively measure protein-protein interactions in vitro or in vivo, monitor protein-protein interactions as they occur in living cells, and determine interaction interfaces. The techniques described utilize a variety of cutting-edge technologies, including surface plasmon resonance (SRP), fluorescence resonance energy transfer (FRET), fluorescence polarization (FP), isothermal titration calorimetry (ITC), circular dichroism (CD), protein fragment complementation assays (PCA), various two-hybrid systems, and proteomics and bioinformatics-based approaches, such as the Scansite program for computational analysis. These powerful tools are essential for deciphering how proteins interact with each other to form biological networks, as well as for unraveling protein-protein interactions in disease to find novel therapeutic targets.

Table of Contents:
Overview.- Structural Basis of Protein-Protein Interactions.- Quantitative Analysis of Protein-Protein Interactions.- In Vitro Techniques.- Characterization of Protein-Protein Interactions by Isothermal Titration Calorimetry.- Circular Dichroism Analysis for Protein-Protein Interactions.- Protein-Protein Interaction Analysis by Nuclear Magnetic Resonance Spectroscopy.- Measuring Rhodopsin-G-Protein Interactions by Surface Plasmon Resonance.- Using Light Scattering to Determine the Stoichiometry of Protein Complexes.- Sedimentation Equilibrium Studies.- Analysis of Protein-Protein Interactions by Simulation of Small-Zone Gel Filtration Chromatography.- Fluorescence Gel Retardation Assay to Detect Protein-Protein Interactions.- Fluorescence Polarization Assay to Quantify Protein-Protein Interactions.- Studying Protein-Protein Interactions via Blot Overlay or Far Western Blot.- Glutathione-S-Transferase-Fusion Based Assays for Studying Protein-Protein Interactions.- Affinity Capillary Electrophoresis Analyses of Protein—Protein Interactions in Target-Directed Drug Discovery.- Mapping Protein-Ligand Interactions by Hydroxyl-Radical Protein Footprinting.- Use of Phage Display and Polyvalency to Design Inhibitors of Protein-Protein Interactions.- Detecting Protein-Protein Interactions in Heterologous Systems.- A Bacterial Two-Hybrid System Based on Transcription Activation.- Using the Yeast Two-Hybrid System to Identify Interacting Proteins.- Analysis of Protein-Protein Interactions Utilizing Dual Bait Yeast Two-Hybrid System.- The Split-Ubiquitin Membrane-Based Yeast Two-Hybrid System.- Reverse Two-Hybrid Techniques in the Yeast Saccharomyces cerevisiae.- Mammalian Two-Hybrid Assay for Detecting Protein-Protein Interactions In Vivo.- Co-Immunoprecipitation from TransfectedCells.- Probing Protein-Protein Interactions in Living Cells.- Microscopic Analysis of Fluorescence Resonance Energy Transfer (FRET).- Monitoring Molecular Interactions in Living Cells Using Flow Cytometric Analysis of Fluorescence Resonance Energy Transfer.- Fluorescence Correlation Spectroscopy.- Confocal Microscopy for Intracellular Co-Localization of Proteins.- Mapping Biochemical Networks With Protein-Fragment Complementation Assays.- In Vivo Protein Cross-Linking.- Proteomics-Based Approaches.- Computational Prediction of Protein-Protein Interactions.- Affinity Methods for Phosphorylation-Dependent Interactions.- Two-Dimensional Gel Electrophoresis for Analysis of Protein Complexes.- Sample Preparation of Gel Electrophoretically Separated Protein Binding Partners for Analysis by Mass Spectrometry.- Quantitative Protein Analysis by Solid Phase Isotope Tagging and Mass Spectrometry.- Internet Resources for Studying Protein-Protein Interactions.

Review :
From the reviews: "This book is thorough and packed with information...the volume is of high practical value and is fully recommended to all biochemists..." - Cell Biochemistry and Function "The book is well-balanced and easy to use...well worth the purchasing cost." - ChemBioChem "This ... volume comprehensively describes a variety of biophysical-, cell biology- and molecular biology-based approaches to the analysis of protein-protein interactions. ... This volume is recommended as a solid presentation on the fundamentals of protein interaction analysis, suitable for graduate biophysical chemistry courses and for those investigators new to the field who would like to get a solid grounding ... ." (Wayne F. Patton, Proteomics, Issue 6, 2006)