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Home > Medicine & Health Science textbooks > Surgery > Wound Healing: Methods and Protocols(78 Methods in Molecular Medicine)
Wound Healing: Methods and Protocols(78 Methods in Molecular Medicine)

Wound Healing: Methods and Protocols(78 Methods in Molecular Medicine)


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About the Book

Expert researchers and physicians not only describe classic and contemporary laboratory methods for studying tissue repair using a broad range of wound healing models, but also provide sensitive assays for the assessment of tissue healing. The systems detailed include human, animal, in vitro, in vivo, and impaired healing models, as well as models in which underlying systemic and genetic conditions influence the healing process. These powerful models illustrate the biochemical, molecular, and surgical techniques designed for the analysis and manipulation of the healing wound, and provide step-by-step instructions to ensure robust results.

Table of Contents:
Experimental Models of Wound Healing.- Excisional Wound Healing.- Methods in Reepithelialization.- Incisional Wound Healing.- Animal Models of Ischemic Wound Healing.- Corneal Injury.- Subcutaneous Sponge Models.- A Mouse Model of Burn Wounding and Sepsis.- A Porcine Burn Model.- Wound Healing in Airways In Vivo.- Murine Models of Intestinal Anastomoses.- Murine Model of Peritoneal Adhesion Formation.- Methods for Investigating Fetal Tissue Repair.- Growth of Human Blood Vessels in Severe Combined Immunodeficient Mice.- Tissue Repair in Models of Diabetes Mellitus.- Wound Healing Studies in Transgenic and Knockout Mice.- Wound Repair in Aging.- Specimen Collection and Analysis.- Suction Blister Model of Wound Healing.- Implantable Wound Healing Models and the Determination of Subcutaneous Collagen Deposition in Expanded Polytetrafluoroethylene Implants.- The Fibroblast-Populated Collagen Lattice.- A Quantifiable In Vitro Model to Assess Effects of PAI-1 Gene Targeting on Epithelial Cell Motility.- Human Skin Organ Culture.- In Vitro Matrigel Angiogenesis Model.- Analysis and Manipulation of Wound Healing.- Quantification of Wound Angiogenesis.- In Vivo Matrigel Migration and Angiogenesis Assays.- Endothelial Cell Migration Assay.- Analysis of Collagen Synthesis.- Method for Detection and Quantitation of Leukocytes During Wound Healing.- Detection of Reactive Oxygen Intermediate Production by Macrophages.- Measurement of Chemokines at the Protein Level in Tissue.- Methods of Measuring Oxygen in Wounds.- Isolation, Culture, and Characterization of Human Intestinal Smooth Muscle Cells.- Use of High-Throughput Microarray Membranes for cDNA Analysis of Cutaneous Wound Repair.- Particle-Mediated Gene Therapy of Wounds.

Review :
From the reviews: "Clinical and basic science researchers with an interest in wound healing of various kinds are an appropriate audience for this work. Editors and authors represent some of the leading wound healing laboratories in the United States with a smaller number of presentations from overseas. . .Chapters are written in a straightforward manner. . .This book is a collection of techniques and insights which belongs on the shelf of laboratories or research organizations having an interest in wounds." -Doody's Health Sciences Book Review Journal "This informative volume provides a highly practical collection of widely used model systems and methods for studying the injury repair process and is therefore highly recommended as a valuable reference source for all individuals with interests in wound healing." - Carbohydrate Polymers "The editors have done an impressive job of editing the book into both a practical and readable style. It is highly recommended for any group that wishes to participate in this exciting and expanding field of research." -British Journal of Surgery "This book … provides a range of classical and more recent tools in wound research. … an impressive collection of both animal and in vitro-based techniques useful for experimental studies of tissue damage and repair. … Each chapter has a concluding notes section offering valuable suggestions and warnings based on practical experience. The book is highly recommended for all basic and clinical researchers involved in tissue repair biology and particularly those with an interest in experimental wound modelling and analysis." (Ola Rollman, Acta Dermato-Venereologica, Vol. 85, 2005)


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Product Details
  • ISBN-13: 9781617372964
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Height: 229 mm
  • No of Pages: 468
  • Series Title: 78 Methods in Molecular Medicine
  • Width: 152 mm
  • ISBN-10: 161737296X
  • Publisher Date: 09 Nov 2010
  • Binding: Paperback
  • Language: English
  • Returnable: Y
  • Sub Title: Methods and Protocols


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