Morphology Methods
Home > Medicine & Health Science textbooks > Medical specialties, branches of medicine > Pathology > Morphology Methods: Cell and Molecular Biology Techniques
Morphology Methods: Cell and Molecular Biology Techniques

Morphology Methods: Cell and Molecular Biology Techniques


     0     
5
4
3
2
1



International Edition


X
About the Book

Ricardo Lloyd, md, phd, provides critical and tutorial surveys of the cutting-edge molecular and cell biologic techniques used today in the analysis of cells and tissues in situ. These molecular-based histologic and morphologic techniques are described in step-by-step detail by hands-on experts, often those who have originated or perfected them. Among the techniques presented are laser capture microdissection, in situ hybridization, FISH, confocal laser scanning microscopy, in situ PCR, and tyramide amplification. Applications include the detection of infectious agents and DNA, the diagnosis of undifferentiated tumors and endocrine lesions, the metaphase analysis of whole blood cultures, and clonality analysis. In its integrated, state-of-the-art approach, Morphology Methods: Cell and Molecular Biology Techniques thoroughly details and applies the rich panoply of significant new cell and molecular biologic technologies to all the major problems confronting morphologic studies and diagnostic pathology today.

Table of Contents:
1 Introduction to Molecular Methods.- 2 Laser Capture Microdissection: Principles and Applications.- Protocol: H&E Staining.- Protocol: Operating the PIX Cell System.- Protocol: DNA Extraction from Collected Cells.- Protocol: RNA Extraction from Collected Cells.- 3 In Situ Hybridization: Detection of DNA and RNA.- Protocol: In Situ Hybridization.- 4 In Situ Detection of Infectious Agents.- Protocol: Detecting Adenovirus.- Protocol: Epstein-Barr Virus (Eber I/II).- 5 Fluorescent In Situ Hybridization.- Protocol: FISH.- 6 Practical Applications of the FISH Technique.- Protocol: Metaphase Analysis from Whole Blood Culture.- Protocol: Metaphase Analysis from Solid Tumors.- Protocol: Interphase Analysis on Cell Imprints—Cytology Specimens.- Protocol: Interphase Analysis of Formalin-Fixed Paraffin-Embedded Tissues.- 7 Tyramide Amplification Methods for In Situ Hybridization.- Protocol: TSA by Rapp et al., 1995.- Protocol: CARD/TSA with Nanogold.- Protocol: Tyramide-enhanced FISH with Peroxidase-labeled Oligonucleotides.- 8 Protocols: Ultrastructural In Situ Hybridization.- 9 Quantitation of In Situ Hybridization Analysis.- 10 Protocols: Confocal Laser Scanning Microscopy.- 11 Protocols: Polymerase Chain Reaction.- 12 Protocols: Detection of Nucleic Acids in Cells and Tissues by In Situ Polymerase Chain Reaction.- 13 Immunohistochemistry: Theory and Practice.- 14 Antigen Retrieval Technique for Immunohistochemistry: Principles, Protocols, and Further Development.- Protocol: Antigen Retrieval (AR) Technique.- 15 Tyramide Amplification in Immunohistochemistry.- Protocol: CARD Immunohistochemistry.- 16 Application of Immunohistochemistry in the Diagnosis of Lymphoid Lesions.- 17 Applications of Immunohistochemistry in the Diagnosis of Undifferentiated Tumors.- 18 Applicationsof Immunohistochemistry in the Diagnosis of Endocrine Lesions.- 19 Ultrastructural Immunohistochemistry.- Protocol: Ultrastructural Immunohistochemistry.- 20 Protocols: Clonality Analysis.

Review :
"The book nicely covers the hot topics in this area -- laser capture microdissection, in situ hybridization, FISH, tyramide amplification methods, etc. An attractive feature of this book is that the last 10 chapters (of the 20) contain actual "cookbook" protocols (e.g., PCR, confocal laser scanning microscopy, immunohistochemistry, antigen retrieval, etc.) that can be adapted immediately to any laboratory...This book would be useful for those needing a concise reference for how to perform modern molecular tissue-based technology. " -Doody's Health Science Book Review Journal "This book describes in detail new methods employed in the study of molecular morphology as a basis for the study of human genes and the etiology of diseases. Among the methods are in situ hybridization, in situ detection of infectious agents, the "FISH" technique, PCR, immunocytochemistry, etc. This excellent production has many photos, some in color, and tables and figures. Very useful to those wishing to employ these techniques." - Journal of Pediatric Endocrinology and Metabolism


Best Sellers


Product Details
  • ISBN-13: 9781617372735
  • Publisher: Humana Press Inc.
  • Binding: Paperback
  • Language: English
  • Returnable: N
  • Width: 178 mm
  • ISBN-10: 1617372730
  • Publisher Date: 09 Dec 2010
  • Height: 254 mm
  • No of Pages: 422
  • Sub Title: Cell and Molecular Biology Techniques


Similar Products

Add Photo
Add Photo

Customer Reviews

REVIEWS      0     
Click Here To Be The First to Review this Product
Morphology Methods: Cell and Molecular Biology Techniques
Humana Press Inc. -
Morphology Methods: Cell and Molecular Biology Techniques
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

Morphology Methods: Cell and Molecular Biology Techniques

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Bookswagon (the "CRR Service").


    By submitting any content to Bookswagon, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Bookswagon (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Bookswagon a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Bookswagon may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Bookswagon's sole discretion. Bookswagon reserves the right to change, condense, withhold publication, remove or delete any content on Bookswagon's website that Bookswagon deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Bookswagon does not guarantee that you will have any recourse through Bookswagon to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Bookswagon reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Bookswagon, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Bookswagon, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept

    New Arrivals


    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!