About the Book
Leading experts detail proven laboratory techniques for the study of MMPs. The methods include those for the expression and purification of MMPs and TIMPs, for the detection of MMPs and TIMPs at both the protein and mRNA levels, and for the assay of MMP and TIMP activities in a wide variety of circumstances. Each method includes step-by-step instructions and notes variant applications and pitfalls to avoid. A selective overview of the MMP arena spells out where the field has been, where it is, and where it is going. Comprehensive and highly practical, Matrix Metalloproteinase Protocols brings together the long and hard-earned experience of master experimentalists that will allow not only novices to get up to speed quickly, but also add to the repertoire of successful techniques in expert laboratories.
Table of Contents:
Part I. MMPs and TIMPs-An Overview of the Field. MMPs and TIMPs: An Historical Perspective, J. Frederick Woessner, Jr. Strategies for Cloning New MMPs and TIMPs, Gloria Velasco and Carlos Lopez-Otin. Structural Studies of MMPs and TIMPs, Wolfram Bode and Klaus Maskos. Matrix Metalloproteinase Substrate Binding Domains, Modules and Exosites: Overview and Experimental Strategies, Christopher M. Overall. The Matrix Metalloproteinase (MMP) and Tissue Inhibitor of Metalloproteinase (TIMP) Genes: Transcriptional and Posttranscriptional Regulation, Signal Transduction and Cell-Type-Specific Expression, Matthew P. Vincenti. Models for Gain-of-Function and Loss-of-Function of MMPs: Transgenic and Gene Targeted Mice, Lisa M. Coussens, Steven D. Shapiro, Paul D. Soloway, and Zena Werb. Part II. Expression and Purification of MMPs and TIMPs. Expression of MMPs and TIMPs in Mammalian Cells, Karen M.-L. Yeow, Blaine W. Phillips, P. Paul Beaudry, Kevin J. Leco, Gillian Murphy, and Dylan R. Edwards. Expression of Recombinant Matrix Metalloproteinases in Escherichia coli, L. Jack Windsor and Darin L. Steele. Expression of Human Collagenase I (MMP-1) and TIMP-1 in a Baculovirus-Based Expression System, Rudiger Vallon and Peter Angel. Expression of Recombinant Matrix Metalloproteinases in Yeast, Glenn A. Doyle. Expression of Recombinant Membrane-Type MMPs, Michael J. Butler, Marie-Pia d'Ortho, and Susan J. Atkinson. Refolding of TIMP-2 from Escherichia coli Inclusion Bodies, Richard A. Williamson. Expression and Refolding of Full-Length Human TIMP-1, Deborah Davis. Purification of MMPs and TIMPs, Ken-ichi Shimokawa and Hideaki Nagase. Part III. Detection of MMPs and TIMPs. Monitoring MMP and TIMP mRNA Expression by RT-PCR, Howard Wong, Huong Muzik, Lori L. Groft, Marc A. Lafleur, Charles Matouk, Peter A. Forsyth, Gilbert A. Schultz, Steven J. Wall, and Dylan R. Edwards. Measuring Transcription of Metalloproteinase Genes: Nuclear Run-Off Assay vs Analysis of hnRNA, Anne M. Delany. In Situ Hybridization for Metalloproteinases and Their Inhibitors, Tina L. Hurskainen and Suneel S. Apte. Use of EIA to Measure MMPs and TIMPs, Noboru Fujimoto and Kazushi Iwata. Immunohistochemistry of MMPs and TIMPs, Yasunori Okada. Detecting Polymorphisms in MMP Genes, Shu Ye and Adriano M. Henney. Part IV. Assay of MMP and TIMP Activities. Methods for Studying Activation of Matrix Metalloproteinases, Vera Knauper and Gillian Murphy. Assay of Matrix Metalloproteinases Against Matrix Substrates, Timothy E. Cawston, Paul Koshy, and Andrew D. Rowan. Zymography and Reverse Zymography for Detecting MMPs and TIMPs, Susan P. Hawkes, Hongxia Li, and Gary T. Taniguchi. In Situ Zymography, Sarah J. George and Jason L. Johnson. Detection of Focal Proteolysis Using Texas-Red-Gelatin, Rosalind M. Hembry. Antibodies to MMP-Cleaved Aggrecan, Amanda J. Fosang, Karena Last, David C. Jackson, and Lorena Brown. Cartilage Proteoglycan Release Assay, Caron J. Billington. Immunoassay for Collagenase-Mediated Cleavage of Types I and II Collagens, R. Clark Billinghurst, Mirela Ionescu, and A. Robin Poole. Collagen Degradation Assays, Anthony P. Hollander. Invasion Assays and Matrix Metalloproteinases: Quantification of Cellular Invasion Using Propidium Iodide Labeling and Confocal Laser Scanning Microscopy, Ulrike Benbow, Kenneth A. Orndorff, and Alice L. Givan. Using Fluorogenic Peptide Substrates to Assay Matrix Metalloproteinases, Gregg B. Fields. Kinetic Analysis of the Inhibition of Matrix Metalloproteinases by Tissue Inhibitor of Metalloproteinases (TIMP), Mike Hutton and Frances Willenbrock. Assaying Growth Factor Activity of Tissue Inhibitors of Metalloproteinases, Kyoko Yamashita and Taro Hayakawa. Index.
Review :
"The editor, Ian M. Clark, has done an excellent job in putting together a collection of articles that span what is now a fairly wide field. ...the content and writing are of a high standard...In chapter 3, covering structural studies and contributed by Wolfram Bode and Klaus Maskos, the authors point out the absence of specific discussions of three-dimensional structures amongst the plethora of general reviews on MMPs and TIMPs. Their contribution expertly fills that void...Indeed the 'Notes' section, at the end of each chapter, is where the benefit of much, often hard-earned, experience is to be reaped and is a valuable addition in almost every case. The last two sections, between them, cover detection of MMp and TIMP expression at both RNA and protein levels, and actively assays. Hore, particular mention must be made of the chapter dealing with zymography and reverse zymography in which Susan Hawkes and coauthors have carefully explained this technique. Their use of figures with detailed interpretation is really excellent and alone could justify the purchase of this book for many labs...this book is recommended as a reference text for all labs engaged in MMP research. There is enough variety for established research labs to find some new methods, and, for newcomers to the field, it should make working with MMPs and their inhibitors a very straightforward prospect." - TRENDS in Cell Biology
