About the Book
Kerry S. Campbell and Marco Colonna have assembled a comprehensive collection of readily reproducible methods designed to study natural killer (NK) cells from the broadest variety of viewpoints. These include not only classic techniques, but also new approaches to standard methods, newly evolved techniques that have become valuable for specific applications, and unique models for manipulating and studying NK cells. Among the advanced methods covered are those for in vitro transendothelial migration, in vivo detection of cells migrating into tumors, immunofluorescence staining of intracellular cytokines, and in vitro NK cell development. Valuable techniques for specific applications include vaccinia virus protein expression, soluble KIR-Fc fusions for HLA class I binding assays, calcium mobilization in cell conjugates, and identification of heterodimeric receptor complexes using cDNA library expression cloning.
Natural Killer Cell Protocols: Cellular and Molecular Methods offers immunologists, cancer researchers, virologists, and cell biologists today's most comprehensive collection of both established and cutting-edge techniques, methods that will contribute significantly to advancing our understanding of this fascinating and critically important class of cells.
Table of Contents:
Cloning and Culturing of Human Natural Killer Cells, Marina Cella and Marco Colonna. Generation of Short-Term Murine Natural Killer Cell Clones to Analyze Ly49 Gene Expression, Werner Held, Bente Lowin-Kropf, and David H. Raulet. Cloning and Culturing of Fetal Mouse Natural Killer Cells, Colin G. Grooks. Techniques for Studying Development of Human Natural Killer Cells and T Cells, Hergen Spits, Pieter Res, and Ana-Cristina Jaleco. Murine Natural Killer Cell Cloning from Fetal Thymic Organ Cultures, Zuhair K. Ballas and Wendy L. Rasmussen. Generation of HLA Class I Transfected Target Cell Lines, Jenny E. Gumperz. Murine Natural Killer Cells and Hybrid Resistance to Hemopoietic Cells in vivo, Jingxuan Liu, Thaddeus George, Gene A. Devora, P. V. Sivakumar, Colleen Davenport, Wayne C. Lai, John Schatzle, Vinay Kumar, and Michael Bennett. Hybrid Resistance by Mouse Natural Killer Cells in vitro, P. V. Sivakumar, Thaddeus George, Igor J. Puzanov, Noelle S. Williams, Susan Stepp, Jingxuan Liu, John Schatzle, Wayne C. Lai, Michael Bennett, and Vinay Kumar. Use of Activated Natural Killer Cells for Tumor Immunotherapy in Mouse and Human, Per H. Basse, Theresa L. Whiteside, and Ronald B. Herberman. Methods for in vivo Analyses of Natural Killer (NK) Cells, William H. Chambers, Simon M. Watkins, and Per H. Basse. Assay for the Transendothelial Migration of Human Natural Killer Cells, Miriam E. Berman and William A. Muller. Methods for Analyzing Chromatin Changes Accompanying Apoptosis of Target Cells in Killer Cell Assays, Richard C. Duke. Methods for Detecting Lytic Granules in Natural Killer Cells, Alison Darmon, Lesley J. Page, and Gillian M. Griffiths. Fluorogenic Substrates as Detectors of Caspase Activity During Natural Killer Cell-Induced Apoptosis, Marek Los, Henning Walczak, and John C. Reed. Techniques for Studying Murine Natural Killer Cells in Defense Against Viral Infection, Anthony A. Scalzo, Helen E. Farrell, and Gunasegaran Karupiah. Assays for Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) and Reverse ADCC (Redirected Cytotoxicity) in Human Natural Killer Cells, Bice Perussia and Matthew J. Loza. Multiple Color Immunofluorescence for Cytokine Detection at the Single Cell Level, Matthew J. Loza, Jeffrey S. Faust, and Bice Perussia. Generation of Antibodies to Cell Surface Markers on Mature NK Cells, Wayne C. Lai, Ying Zhang, Thadeus George, P. V. Sivakumar, Susan Stepp, Deming Zhou, Michael Bennett, Vinay Kumar, and John Schatzle. Human Single Chain Fv Fragments Specific for Natural Killer Cell Receptors from Phage Display Libraries, Barbara Uchanska-Ziegler, Qiao Xugang, Armin Volz, Ahuva Nissim, and Andreas Ziegler. Binding of Soluble KIR-Fc Fusion Proteins to HLA Class I, Christine C. Winter and Eric O. Long. Genetic Analysis of a Highly Homologous Gene Family: The Killer Cell Immunoglobulin-Like Receptors, Michael J. Wilson, Michaela Torkar, and John Trowsdale. Use of Vaccinia Virus for Functional Gene Transfer in Human Natural Killer Cells, Sumati Rajagopalan and Eric O. Long. Use of cDNA Library Expression Cloning to Identify Components of Heterodimeric Receptor Complexes, Chiwen Chang and Lewis L. Lanier. Functional Analysis of NK Cell Receptors in the RNK-16 Rat Leukemic Cell Line, James C. Ryan, Erene C. Niemi, and Mary C. Nakamura. Use of Mutant Jurkat T Cell Lines to Define Human Natural Killer Cell Surface Receptor Signaling Pathways, Bryce A. Binstadt, Brandi L. Williams, Robert T. Abraham, and Paul J. Leibson. Measurement of Calcium Mobilization Responses in Killer Cell/Target Conjugates by FACS Analysis, Salvatore Valitutti and Mark Dessing. BIAcore Analysis to Test Phosphopeptide-SH2 Interactions, Frederic Vely, Alain Trautmann, and Eric Vivier. Analysis and Use of Mutant Mice Exhibiting Natural Killer Cell Defects, Toru Miyazaki and Kerry S. Campbell. Isolation and Analysis of Natural Killer Cells in Chickens, Thomas W. F. Gobel. Analyzing Nonspecific Cytotoxic Cells in Fish, Sandra Ristow, Donald L. Evans, and Liliana Jaso-Friedmann. Natural Killer Cells and Immunotoxicology, Albert E. Munson and Kathleen E. Phillips. Appendix. Index
Review :
"Natural Killer Cell Protocols is a worthwhile addition to the reference collection of an academic library that serves scientists who are conducting research on NK cells, lymphocytes, cancer, and/or viral infections. It is well representative of the previous volumes in the Methods in Molecular Biology series and therefore is an important acquisition for libraries collecting the series in its entirety."-E-Streams (Electronic Reviews of Science and Technology References "Besides clearly describing each step in the assays, the very detailed protocols point out potential trouble spots and provide those small points that make a technique workable. This is a fine "cookbook" to use to get started on NK cell assays."-Doody's Health Science Book Review Journal "The book contains 31 chapters contributed by experts in the field...The chapters are generally well written and informative. Experimental procedures are presented in great detail, which should be very helpful for those who are unfamiliar with the methodologies involved. An especially useful feature is the inclusion of notes at the end of each chapter in which potential problems and pitfalls are describe and methods for dealing with them are suggested. The appendices provide a handy summary of monoclonal antibodies and cell lines that are commonly used in NK cell studies...this book is an excellent compendium of laboratory methods relevant to research on NK cells. It should prove valuable not only to novices embarking upon NK cell work, but also to experienced investigators who wish to explore a new facet of these intriguing lymphocytes." - Hematologic Oncology
