Cell-Penetrating Peptides: Methods and Protocols(683 Methods in Molecular Biology)

In recent years, a new understanding of cell-penetrating peptides has emerged, helping researchers to expand beyond a number of long-held dogmas. In Cell-Penetrating Peptides: Methods and Protocols, expert researchers explore the latest information on cell-penetrating peptides (CPPs), providing insight into the most important and contemporary areas of CPP research. Chapters address the historical background of CPP studies, provide an overview of the growing field of research, investigate methods for testing CPP mechanisms, present a summary of methods that attempt to use properties of CPPs to study biochemical intracellular mechanisms of interaction and signal transduction, and include new ideas for turning CPP-based strategies into drugs. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Innovative and current, Cell-Penetrating Peptides: Methods and Protocols is an essential guide that allows researchers to study intracellular mechanisms in new ways, and to promote the future development of novel drugs.

Table of Contents:
Classes and prediction of cell-penetrating peptides.- Penetratin story : an overview.- Testing membrane interactions of CPPs.- Interactions of amphipathic CPPs with model membranes.- NMR studies of three-dimensional structure and positioning of CPPs in membrane model systems.- Measurements of the intracellular stability of CPPs.- Tools for predicting binding and insertion of CPPs into lipid bilayers.- Studies of proteoglycan involvement in CPP-mediated delivery.- Uptake Kinetics of cell-penetrating peptides.- Thermodynamics of lipid interactions with cell-penetrating peptides.- Calcium and membrane repair.- Mapping of protein transduction pathways with fluorescent microscopy.- Insight into cell-entry mechanisms of CPPs by electron microscopy.- Toxicity methods for CPPs.- Comparison of CPP uptake methods.- Characterization of cellular internalization pathways for CPP-mediated oligonucleotide delivery.- Mimicry of protein function with cell-penetrating peptides.- Homeoprotein intercellular transfer, the hidden face of cell-penetrating peptides.- Pharmacology, biodistribution, and efficacy of GPCR-based pepducins in disease models.- Identification and Characterization of Tissue-Specific Protein Transduction Domains using Peptide Phage Display.- Applications of Cell Penetrating Peptides as Signal Transduction Modulators for the Selective Induction of Apoptosis.- Splice Redirection as a Convenient Assay to Monitor CPP-ON Efficiency and Mechanism.- CPP directed oligonucleotide exon skipping in animal models of Duchenne muscular dystrophy.- PTD-DRBD siRNA delivery.- A non-covalent peptide-based strategy for siRNA delivery.- Application of PepFect peptides for the delivery of splice correcting oligonucleotides.- Internalization of nucleoside phosphates into live cells by complex formation with different CPPs and JBS-Nucleoducin.- Enhanced cellular delivery of cell-penetrating peptide –peptide nucleic acid conjugates by photochemical internalization (PCI).- Identificationof homing peptides using the in vivo phage display technology.- Measuring the action of CPP-siRNA conjugates in the lung.- Intracellular delivery of nanoparticles with CPPs.- Multifunctional CPP polymer system for tumor-targeted pDNA and siRNA delivery.- Cell Penetrating Penta-Peptides (CPP5s) and Bax Inhibiting Peptides (BIPs): Protocol for their application.- PAIR technology: exon-specific RNA binding protein isolation in live cells.- Quantitation of cellular and topical uptake of luciferin oligoarginine conjugates.- Industrial-scale manufacturing of a possible oligonucleotide cargo CPP based drug.- Application of a fusiogenic peptide GALA for intracellular delivery.- Therapeutic Applications of Cell-Penetrating Peptides.- Nonclinical and clinical experiences with CPP-based self assembling peptide systems in topical drug development.

Review :
From the reviews: “Prof. Ulo Langel … rightly explain in a necessary preface that there are in use so many definition for the same things: protein transduction domain (PTDs), Trojan peptides, model amphipathic peptides (MAPs), membrane translocating sequences (MTS) that the best way to refer to all of these molecules is to call all of them cell-penetrating peptides, CPPs. … Clearly enough, the cell-penetrating peptides volume is surely of great help for basic research and for clinicians engaged in translational medicine.” (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 55, 2011)