In Vitro Transcription and Translation Protocols
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In Vitro Transcription and Translation Protocols: (v. 375 Methods in Molecular Biology)

In Vitro Transcription and Translation Protocols: (v. 375 Methods in Molecular Biology)


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About the Book

An update of the previous edition, "In Vitro Transcription and Translation Protocols" provides molecular biology laboratories with the most powerful techniques for employing in vitro transcriptions and translation systems. Consisting of sections focusing on technology and applications, its topics include continuous-exchange protein synthesizing systems, energy systems for ATP regeneration in cell-free protein synthesis reactions, as well as examining in vitro translation to study HIV protease activity.

Table of Contents:
Section One: TECHNOLOGIES; Chapter One - Energy Systems for ATP Regeneration in Cell-free Protein Synthesis Reactions; Kara A. Calhoun, James R. Swartz; Chapter 2 - Continuous-exchange Protein-synthesizing Systems; Vladimir A. Shirokov, Aigar Kommer, Vyacheslav A. Kolb, Alexander S. Spirin; Chapter 3 - Cell-free production of integral membrane proteins on a preparative scale"; Christian Klammt, Daniel Schwarz, Vilker Dotsch, Frank Bernhard; Chapter 4- SIMPLEX: Single-molecule PCR-linked in vitro expression: a novel method for high-throughput construction and screening of protein libraries; Suang Rungpragayphan, Tsuneo Yamane, Hideo Nakano; Chapter 5 - Methods for High-throughput Materialization of Genetic Information Based on Wheat Germ Cell-free Expression System; Tatsuya Sawasaki, Ryo Morishita, Mudeppa D. Gouda, Yaeta Endo; Chapter 6 - Exogenous Protein Expression in Xenopus Oocytes: Basic Procedures; Elena Bossi, Maria Serena Fabbrini, Aldo Ceriotti; Section Two: APPLICATIONS; Chapter 7 -In vitro translation to study HIV protease activity; Zene Matsuda, Mutsunori Iga, Kosuke Miyauchi, Jun Komano, Kazuhiro Morishita, Akihiko Okayama, Hirohito Tsubouchi; Chapter 8 - The protein truncation test in mutation detection and molecular diagnosis; Oliver Hauss, Oliver Muller; Chapter 9 - Creation of Novel Enantioselective lipases by SIMPLEX; Yuichi Koga, Tsuneo Yamane, Hideo Nakano; Chapter 10 - In vitro transcription and translation coupled to 2D-electrophoresis for bacterial proteome analysis; Nathalie Norais, Ignazio Garaguso, Germano Ferrari, Guido Grandi; Chapter 11 - In vitro Screen of Bioinformatically Selected Bacillus anthracis Vaccine Candidates by Coupled Transcription, Translation and Immunoprecipitation Analysis; Orit Gat, Haim Grosfeld, Avigdor Shafferman; Chapter 12 - Functional expression of type 1 rat GABA transporter in microinjected Xenopus laevis oocytes. Stefano Giovannardi, Simona Magagnin, Andrea Soragna, Laura Faravelli; Chapter 13 - Production of Protein for NMR Study Using the Wheat Germ Cell-Free System; Toshiyuki Kohno, Yaeta Endo.


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Product Details
  • ISBN-13: 9781597453882
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Edition: Revised edition
  • Language: English
  • Width: 152 mm
  • ISBN-10: 1597453889
  • Publisher Date: 18 Jun 2014
  • Binding: Digital (delivered electronically)
  • Height: 229 mm
  • Series Title: v. 375 Methods in Molecular Biology


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