Pyrosequencing Protocols by Sharon Marsh at Bookstore UAE
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Pyrosequencing Protocols: (373 Methods in Molecular Biology)

Pyrosequencing Protocols: (373 Methods in Molecular Biology)


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About the Book

Within "Pyrosequencing Protocols", the protocols for utilizing Pyrosequencing technology are described in detail, including trouble-shooting tips and background information. Chapter 1 provides an introduction to the fascinating origins of the methodology. Chapter 2 provides a brief overview of some of the applications Pyrosequencing is used for. Chapters 3 and 4 describe primer selection and the basic technique. Chapters 5 through 7 provide methods for improving the throughput and decreasing the cost of Pyrosequencing. Detailed applications for the technique can be ound in Chapters 8-13, whilist the important aspect of data storage is discussed in Chapter 14.

Table of Contents:
Table of contents; Chapter 1: The History of Pyrosequencing[registered]. Pal Nyren; Chapter 2: Pyrosequencing[registered] applications. Sharon Marsh; Chapter 3: Web-Based Primer Design Software for Genome Scale Genotyping by Pyrosequencing[registered]. Steven Ringquist, Christopher Pecoraro, Ying Lu, Alexis Styche, William A. Rudert, Panayiotis V. Benos and Massimo Trucco; Chapter 4: Pyrosequencing[registered]: A Simple Method for Accurate Genotyping. Cristi R. King and Tiffany Scott-Horton; Chapter 5: Universal Primer Applications for Pyrosequencing[registered]. Dong-chuan Guo and Dianna M. Milewicz; Chapter 6: Allele Quantification and DNA Pooling Methods. Jonathon Wasson; Chapter 7: Multiplex Pyrosequencing[registered] for DNA Variation Analysis. Pritesh Patel, Yih-Horng Shiao, and Paolo Fortina; Chapter 8: Analysis of gene-specific DNA methylation patterns by the Pyrosequencing[registered] technology. Jorg Tost and Ivo Glynne Gut; Chapter 9: Detecting mutations that confer oxazolidinone resistance in Gram-positive bacteria. Neil Woodford, Sarah E. North and Matthew J. Ellington; Chapter 10: Pyrosequencing[registered] Based Strategies for Improved Allele Typing of HLA Loci. Steven Ringquist, Alexis Styche, William A. Rudert, and Massimo Trucco; Chapter 11: Pyrosequencing[registered] of Phage Display Libraries for the Identification of Cell Specific Targeting Ligands. Ahad A. Rahim; Chapter 12: Gene Copy Number Detection in Animal Studies. Gerli Pielberg and Leif Andersson; Chapter 13: Detection of Allelic Imbalance in Gene Expression Using Pyrosequencing[registered]. Hua Wang and Steven C. Elbein; Chapter 14: Pyrosequencing[registered] Genotype Storage Techniques. Derek J. Van Booven.


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Product Details
  • ISBN-13: 9781597453776
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Height: 229 mm
  • Width: 152 mm
  • ISBN-10: 1597453773
  • Publisher Date: 01 Nov 2006
  • Binding: Digital (delivered electronically)
  • Series Title: 373 Methods in Molecular Biology


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