Cryopreservation and Freeze-drying Protocols
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Cryopreservation and Freeze-drying Protocols: (368 Methods in Molecular Biology)

Cryopreservation and Freeze-drying Protocols: (368 Methods in Molecular Biology)

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About the Book

This book, in addition to outlining the fundamental principles associated with the conservation of Biological Resources, freeze-drying and cryopreservation, is a compilation of cryopreservation and freeze-drying methodologies applicable to different biological materials, which have been developed by expert laboratories. The protocols are reproducible, robust, and most have been transferred successfully to other laboratories. "Cryopreservation and Freeze-Drying Protocols, Second Edition" expands on the range of materials previously covered and includes many novel approaches and protocols for biological materials that were not preservable at the time.

Table of Contents:
Contents; Preface; Contributors; Chapter 1; Long-term ex situ conservation of Biological Resources and the role of Biological Resource Centres; Glyn N. Stacey and John G. Day; Chapter 2; The Process of Freeze-drying; Gerald Adams; Chapter 3; Principles of Cryopreservation; David E. Pegg; Chapter 4; Lyophilization of Proteins; Paul Matejschuk; Chapter 5; Vacuum-drying and Cryopreservation of Prokaryotes; Brian J. Tindall; Chapter 6; Freeze-drying of Yeast Cultures; Chris Bond; Chapter 7; Cryopreservation of Yeast Cultures; Chris Bond; Chapter 8; Freeze-drying Fungi using a Shelf-freeze-drier; C. Shu-hui Tan, Cor W. van Ingen and Joost A. Stalpers; Chapter 9; Cryopreservation and Freeze-drying of Fungi employing Centrifugal and Shelf Freeze-drying; Matthew J. Ryan and David Smith; Chapter 10; Cryopreservation of Microalgae and Cyanobacteria; John G. Day; Chapter 11; Cryopreservation of Plant Cell Suspensions; Brian W. W. Grout; Chapter 12; Cryopreservation of Shoot-tips and Meristems; Erica E. Benson, Keith Harding and Jason W. Johnston; Chapter 13. Cryopreservation of Desiccation Tolerant Seeds; Hugh W. Pritchard; Chapter 14; Cryopreservation of Fish Sperm; Eugeny Kopeika, Julia Kopeika and Tiantian Zhang; Chapter 15; Cryopreservation of Avian Spermatozoa; Graham Wishart; Chapter 16; Cryopreservation of Animal and Human Cell Lines; Christopher B. Morris; Chapter 17; Cryopreservation of Haematopoietic Stem/Progenitor Cells for Therapeutic Use; Suzanne M. Watt, Eric Austin and Sue Armitage; Chapter 18; Cryopreservation of Human Embryonic Stem Cell Lines; Charles J. Hunt and Paula M. Timmons; Chapter 19; Cryopreservation of Primary Animal Cell Cultures; Glyn N. Stacey and Stuart Dowall; Chapter 20; Cryopreservation of Red Blood Cells and Platelets; Andreas Sputtek; Chapter 21; Cryopreservation of Mammalian Semen; Mark R. Curry; Chapter 22; Cryopreservation of Mammalian Oocytes; Sharon J. Paynter and Barry J. Fuller; Chapter 23; Cryopreservation of Mammalian Embryos; Barry J. Fuller and Sharon J. Paynter; Glossary.


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Product Details
  • ISBN-13: 9781597453622
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Height: 229 mm
  • Width: 152 mm
  • ISBN-10: 1597453625
  • Publisher Date: 14 May 2014
  • Binding: Digital (delivered electronically)
  • Series Title: 368 Methods in Molecular Biology


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