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Home > Mathematics and Science Textbooks > Science: general issues > Scientific equipment, experiments and techniques > Electron Microscopy: Methods and Protocols(v. 369 Methods in Molecular Biology)
Electron Microscopy: Methods and Protocols(v. 369 Methods in Molecular Biology)

Electron Microscopy: Methods and Protocols(v. 369 Methods in Molecular Biology)


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About the Book

Hands-on experts describe in detail the key electron microscopy techniques used for examining cells, tissue, biological macromolecules, molecular structure, and their interactions. "Electron Microscopy Methods and Protocols, Second Edition" offers both newcomers and established researchers across experimental biology and medicine wanting to expand their repertoire a gold-standard laboratory manual of cutting-edge electron microscopy techniques - each optimized for reproducibility and robust results - today's gold-standard laboratory manual. New to this edition are sections covering Transmission Electron Microscopy (TEM), and Scanning Electron Microscopy (SEM).

Table of Contents:
CONTENTS; Electron Microscopy: Methods and Protocols (2nd Edition); "METHODS IN MOLECULAR BIOLOGY" Volume 117; 1. Conventional Specimen Preparation Techniques for Transmission Electron Microscopy of Cultured Cells; John J. Bozzola (USA); 2. Processing Biological Tissues for Ultrastructural Study; Jose A. Mascorro (USA) and John J. Bozzola (USA); 3. Processing Plant Tissues for Ultrastructural Study; John Kuo and Ivan Kuo (Australia); 4. Microwave-Assisted Processing and Embedding for Transmission Electron Microscopy; Paul Webster (USA); 5. Ultramicrotomy: for Biological Electron Microscopy; Herbert K. Hagler (USA); 6. Post Staining Grids for Transmission Electron Microscopy: Conventional and Alternative Protocols; E. Ann Ellis (USA); 7. Negative Staining of Thinly Spread Biological Samples; J. Robin Harris (Germany); 8. Recent Advances in High Pressure Freezing: Equipment and Specimen Loading Methods; Kent L McDonald, Mary Morphew, Paul Verkade, and Thomas Muller-Reichert (USA/Germany); 9. Cryoultramicrotomy: Cryo-Electron Microscopy of Vitreous Sections; Dimitri Vanhecke, Luca Studer and Daniel Studer (Switzerland); 10. Cell-free Extract Systems and the Cytoskeleton: Preparation of Biochemical Experiments for Transmission Electron Microscopy; Margaret Coughlin, William M. Brieher and Ryoma Ohi (USA); 11. Electron Microscopy In Situ Hybridization - Tracking DNA and RNA Sequences at High Resolution; Du_an Cmarko and Karel Koberna (Chezvakea); 12. Correlative Light and Electron Microscopy Using Immunolabeled Resin Sections; Heinz Schwarz and Bruno M. Humbel (Germany/Netherlands); 13. Cryosectioning Fixed and Cryoprotected Biological Material for Immunocytochemistry; Paul Webster and Alexandre Webster (USA); 14. F-TEM mages: Integrating Fluorescence Microscopy with Transmission Electron Microscopy; Paul A. Sims and Jeff D. Hardin (USA); 15. Quantitative Immunoelectron Microscopy: Alternative Ways of Assessing Subcellular Patterns of Gold Labelling; Terry M. Mayhew (England); 16. Electron Crystallography of Membrane Proteins; Hui-Ting Chou, James Evans and Henning Stahlberg (USA). 17. Cryo-Electron Microscopy of Icosahedral Virus Particle; Wen Jiang and Wah Chiu (USA); 18. 3D Reconstructions of Chromosomes with Electron Tomography; Peter Engelhardt (Finland); 19. Whole-Mount Immuno-Electron Tomography of Chromosomes and Cells; Peter Engelhardt, Jari Merilainen, Fang Zhao, Susumu Uchiyama, Kiichi Fukui and Veli-Pekka Lehto (Finland/Japan); 20. 3-D Cryo-Transmission Electron Microscopy of Cells and Organelles; Michael Marko and Chyong-Ere Hsieh (USA); 21. Electron Energy-Loss Spectroscopy (EELS) as a Tool for Elemental Analysis in Biological Specimens; Nadine Kapp, Peter Gehr, Daniel Studer and Marianne Geiser (Switzerland); 22. Conventional Specimen Preparation Techniques for Scanning Electron Microscopy of Biological Specimens; John J. Bozzola (USA); 23. Variable Pressure (VPSEM) and Environmental Scanning Electron Microscopy (ESEM): Imaging of Biological Samples; Brendan J. Griffin (Australia); 24. Cryo-planing Technique for Visualizing the Distribution of Water in Woody Tissue by Cryo-Scanning Electron Microscopy; Yasuhiro Utsumi and Yuzou Sano (Japan); 25. X-ray Microanalysis in the Scanning Electron Microscope; Godfrield M Roomans and Anca Dragomir (Sweden); 26. Botanical SEM X-ray Microanalysis in Cryo-Scanning Electron Microscopy; Beat Frey (Switzerland); 27. Static Secondary Ion Mass Spectrometry for Biological and Biomedical Research; Nicholas P. Lockyer (England); 28. Using SIMS and MIMS in Biological Materials: Application to Higher Plants; Nicole Grignon (France).


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Product Details
  • ISBN-13: 9781597452946
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Edition: Revised edition
  • Series Title: v. 369 Methods in Molecular Biology
  • ISBN-10: 1597452947
  • Publisher Date: 01 Jan 2007
  • Binding: Digital (delivered electronically)
  • Language: English
  • Sub Title: Methods and Protocols


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