Protein Engineering Protocols by Katja Arndt - Bookswagon
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Home > Mathematics and Science Textbooks > Biology, life sciences > Biochemistry > Protein Engineering Protocols: (No. 352 Methods in Molecular Biology)
Protein Engineering Protocols: (No. 352 Methods in Molecular Biology)

Protein Engineering Protocols: (No. 352 Methods in Molecular Biology)


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About the Book

"Protein Engineering Protocols" considers the general, but not mutually exclusive, strategies for protein engineering. The first is known as rational design, in which the scientist uses detailed knowledge of the structure and function of the protein to make desired changes. The second strategy is known as directed evolution. This volume provides a comprehensive guide to the methods used at every stage of the engineering process. It combines a thorough theoretical foundation with detailed protocols and will be invaluable to all research workers in the area, from graduate students to senior investigators.

Table of Contents:
Table of contents for Protein Engineering Protocols:; PART I. DESIGN AND COMPUTATIONAL STRATEGIES FOR PROTEIN ENGINEERING; 1 Combinatorial protein design strategies using computational methods Hidetoshi Kono, Wei Wang and Jefferey G. Saven; 2 Global incorporation of unnatural amino acids in Escherichia coli Jamie M. Bacher and Andrew D. Ellington; 3 Considerations in the Design and Optimization of Coiled coil Structures Jody M. Mason, Kristian M. Muller and Katja M. Arndt; 4 Ca2+ indicators based on Calmodulin - Fluorescent Protein Fusions Kevin Truong, Asako Sawano, Atsushi Miyawaki, Mitsuhiko Ikura; 5 Design and Synthesis of Artificial Zinc Finger Proteins Wataru Nomura and Yukio Sugiura; 6 Monobodies: antibody mimics based on the scaffold of the fibronectin type III domain Akiko Koide and Shohei Koide; 7 Engineering site-specific endonucleases Peter Friedhoff and Alfred Pingoud; PART II. EVOLUTIONARY STRATEGIES FOR PROTEIN ENGINEERING; 8 Protein Library Design and Screening: Working Out the Probabilities Joelle N. Pelletier and Michel Denault; 9 Protein Design By Binary Patterning of Polar and Nonpolar Amino Acids Luke H. Bradley, Yinan Wei, Peter Thumfort, Christine Wurth and Michael H. Hecht; 10 Versatile DNA fragmentation and directed evolution with Nucleotide Exchange and Excision Technology (NExT) Sabine C. Stebel, Katja M. Arndt and Kristian M. Muller; 11 Degenerate Oligonucleotide Gene Shuffling (DOGS); Peter L. Bergquist and Moreland D. Gibbs. 12 M13 Bacteriophage Coat Proteins Engineered for Improved Phage Display Sachdev S. Sidhu, Birte K. Feld and Gregory A. Weiss; 13 Ribosome-Inactivation Display System (RIDS) Satoshi Fujita, Jing-Min Zhou and Kazunari Taira; 14 Compartmentalized self-replication (CSR): a novel method for the directed evolution of polymerases and other enzymes Farid J. Ghadessy and Philipp Holliger; 15 Synthesis of Degenerated Libraries of the RBD of Raf and Rapid Selection of fast-folding and stable clones with the DHFR Protein fragment Complementation Assay Francois-Xavier Campbell-Valois and Stephen W. Michnick; 16 A general method of terminal truncation, evolution and re-elongation to generate enzymes of enhanced stability Jochen Hecky, Jody M. Mason, Katja M. Arndt and Kristian M. Muller.

Review :
From the reviews: "Whether the research in your laboratory focuses on protein engineering or you wish to adopt a method for engineering a protein of interest, you will find this book, with it up-to-date stepwise protocols and experimentally oriented language, well suited to these goals." -Quarterly Review of Biology "The chapters of Protein Engineering Protocols have been written by researchers with years of practical experience at the bench. It represents a methodological cook-book of high quality. The diverse topics are well written and covered in an up-to-date manner. This makes it a bench-top handbook indispensable for all scientists who either work directly in protein engineering and design or use the described approaches of this field for their own research." (Nediljko Budisa, ChemBioChem, Vol. 8, 2007)


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Product Details
  • ISBN-13: 9781597451871
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Language: English
  • ISBN-10: 1597451878
  • Publisher Date: 01 Jan 2007
  • Binding: Digital (delivered electronically)
  • Series Title: No. 352 Methods in Molecular Biology


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