Diagnostic Bacteriology Protocols
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Diagnostic Bacteriology Protocols: (No. 345 Methods in Molecular Biology)

Diagnostic Bacteriology Protocols: (No. 345 Methods in Molecular Biology)


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About the Book

"Diagnostic Bacteriology Protocols" is designed to highlight new technologies of potential use in a diagnostic setting and to outline the technological advances that have recently been made in the field of diagnostic testing. This volume will provide ideas and aid in decision making for those intending to introduce novel identification, detection, or typing technologies into their laboratories. Also included are contributions by scientists at the forefront of diagnostic test development, as well as reviews treating current and future molecular diagnostic tests, and nucleic acid extraction methods.

Table of Contents:
Preface; Contributors; 1 A Review of Current and Future Molecular Diagnostic Tests for Use in the Microbiology Laboratory.; Geert Jannes and Daniel De Vos; 2 Overview of DNA Purification for Nucleic Acid-based Diagnostics from Environmental and Clinical Samples; Knut Rudi and Kjetill S. Jakobsen; 3 Microarray-based Detection of Bacteria by On-chip PCR; Georg Mitterer and Wolfgang M. Schmidt; 4 An Array Biosensor for Detection of Bacterial and Toxic Contaminants of foods; Miriam M. Ngundi and Chris R. Taitt; 5 Detection of Neisseria meningitidis, Streptococcus pneumoniae and Haemophilus influenzae in Blood and Cerebrospinal Fluid using Fluorescence-based PCR; Stuart C. Clarke; 6 Use of Hybridization Probes in a Real-time PCR Assay on the LightCycler[registered] for the Detection of MRSA; Andrea J. Grisold and Harald H. Kessler; 7 Detection of Verotoxin Genes VT 1 and VT 2 in Escherichia coli O157:H7 in Minced Beef using Immunocpture and Real-time PCR.; Justine Fitzmaurice; 8 Application of a Quantitative Two-step qRT-PCR to Bacterial Daignostics.; Barry Glynn; 9 Quencher Extension (QEXT) for Single Nucleotide Polymorphism Quantification in Bacterial Typing and Microbial Community Analyses; Knut Rudi, Monika Zimonja and Beate Skaseng; 10 Amplified Fragment Length Polymorphism and Protein Profiling for Identification of Campylobacter lari Subgroups; Birgitta Duim and Jaap Wagenaar; 11 Use of Peptide Nucleic Acid Probes for Rapid Detection and Enumeration of viable Bacteria in Recreational Waters and Beach Sand; Nwadiuto Esiobu; 12 HOOF-Prints: Brucella Strain Typing by PCR Amplification of Multilocus Tandem Repeat Polymorphisms; Betsy J. Bricker and Darla R. Ewalt; 13 Detection of Legionella in Various Sample Types using Whole Cell Fluorescent in situ Hybridization (FISH); Priscilla Declerck and Frans Ollevier; 14 Identification of Diagnostic Proteins in Mycobacterium avium subspecies paratuberculosis by Whole genome Analysis Approach; John P. Bannantine and Michael L. Paustian; 15 Diagnosois of Q Fever using Indirect Microimmunifluorescence; Guenievre Imbert and Bernard La Scola; 16 Use of Macrophage Cell Cultures for Rapid Isolation of Intracellular Bacteria: Mycobacterium bovis Model; Massimo Amadori, Marco Ritelli, Silvia Tagliabue and Maria L. Pacciarini.


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Product Details
  • ISBN-13: 9781597451437
  • Publisher: Humana Press Inc.
  • Binding: Digital (delivered electronically)
  • Series Title: No. 345 Methods in Molecular Biology
  • ISBN-10: 1597451436
  • Publisher Date: 18 Jun 2014
  • Language: English


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