Agrobacterium Protocols
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Agrobacterium Protocols: v. I

Agrobacterium Protocols: v. I


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About the Book

The second edition of "Agrobacterium Protocols" contains 80 chapters (two volumes) divided into 14 parts. In Volume I, there are a total of six parts. Part I (Agrobacterium handling) provides six chapters describing basic techniques in Agrobacterium manipulation and strategies for vector construction, major components of plant transformation that are often neglected by many plant biologists. Part II (Model plants) consists of seven chapters describing various ways to introduce DNA into three major model plant species, Arabidopsis thaliana, Medicago truncatula, and Nicotiana. Although most plant laboratories transform these model plants on a routine basis, protocols from leading experts may further enhance their capabilities. Parts III through XIII collect 61 chapters covering protocols for 59 plant species. The plants are grouped according to their practical utilization rather than their botanical classification. The significant expansion of this section reflects the remarkable advancements in plant transformation technology during the past decade. Volume I contains four of the eleven parts (Part III to VI) of plant protocols. This book provides a bench-top manual for tested protocols involving Agrobacterium-mediated transformation.

Review :

From the reviews of the second edition:

"This is, as the title suggests, a laboratory manual with protocols for using Agrobacterium tumefaciens to transform plant species . the book will be a valuable addition to the laboratory bookshelf. The protocols are clearly set out and the notes explain things . I was also pleased that some chapters mentioned the need to consider regulatory issues regarding disposal of genetically modified materials, something that is often ignored in lab manuals." (Heather Macdonald, SGM Society for General Microbiology, 2007)


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Product Details
  • ISBN-13: 9781597451307
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Language: English
  • ISBN-10: 1597451304
  • Publisher Date: 14 May 2014
  • Binding: Digital (delivered electronically)
  • Sub Title: v. I


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