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Home > Mathematics and Science Textbooks > Biology, life sciences > Cellular biology (cytology) > Transmembrane Signaling Protocols: (No. 332 Methods in Molecular Biology)
Transmembrane Signaling Protocols: (No. 332 Methods in Molecular Biology)

Transmembrane Signaling Protocols: (No. 332 Methods in Molecular Biology)


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About the Book

Since the previous edition of "Transmembrane Signaling Protocols" (1998), new advances have increased the ability to understand transmembrane signaling processes in greater detail. The human genome has been completely sequenced as well as new methods have been developed for microarrays and proteomics analyzing global changes in gene expression and protein profiles. Updated to include these new methods, "Transmembrane Signaling Protocols, 2nd Edition", also includes comprehensive detailed methods, studies in genetically tractable systems, fluorescence microscopy in live single cells, ex vivo analysis of primary cells from transgenic mice, and genomic and proteomic approaches to the analysis of transmembrane signaling events.

Table of Contents:
Part I: OVER VIEWS:; 1 Transmembrane signaling by G protein coupled receptors; Louis M. Luttrell; 2 Cross-talk coregulation mechanisms of G protein coupled receptors and receptor tyrosine kinases; Kanchana Nataranjan and Bradford C. Berk; 3 Protein-protein Interactions in Signaling Pathways; Bruce J. Mayer; PART II. SPECIFIC TOPICS; A. MEMBRANE RECEPTORS AND SIGNALING; 4 Biological role of the CXCR4-SDF-1 axis in normal human hematopoietic cells; Marcin Majka and Mariusz Z. Ratajczak; 5 Functional Expression of CXCR4 in Saccharomyces Cerevisiae for the Development of Powerful Tools for Pharmacologic Characterization of CXCR4; Zi-xuan Wang, James R. Broach and Stephen C. Peiper; 6 Characterization of constitutively active mutants of G protein coupled receptors; Jean-Marc Navenot, Zi-xuan Wang and Stephen C. Peiper; 7 G-protein coupled receptor dimerization and signaling; Mario Mellado, Antonio Serrano., Carlos Martinez-A and Jose Miguel Rodriguez-Frade; 8 Real time analysis of G-protein coupled receptor signaling in live cells; Venkatakrishna R Jala and Bodduluri Haribabu; B. LIPID RAFT AND CAVEOLAE IN TRANSMEMBRANE SIGNALING; 9 Isolation of membrane lipid rafts and signaling complexes; Kathleen Boesze Battaglia; 10 Methods for the study of signaling molecules in membrane lipid rafts and caveolae; Rennolds S. Ostrom and Paul A. Insel. C. PROTEIN-PROTEIN INTERACTION IN TRANSMEMBRANE SIGNALING; 11 Bioluminescence Resonance Energy Transfer (BRET) to monitor protein-protein interactions; Tarik Issad and Ralf Jockers; 12 Identification of interacting proteins using the yeast two-hybrid screen; Kelly L. Jordan-Sciutto and Marshall B. Montgomery; 13 Analysis of PDZ domain interactions using yeast two-hybrid and coimmunoprecipitation assays; Hyun Woo Lee, Jaewon Ko, and Eunjoon Kim; 14 Clustering assay for studying the interaction of membrane proteins with PDZ domain proteins; Jaewon Ko and Eunjoon Kim; D. RHO GTPASES AND RECONSTITUTION OF SIGNALING COMPLEXES; 15 Mammalian cell microinjection assay to study the function of Rho family GTPases; Ritu Garg and Anne J. Ridley; 16 Affinity based assay of Rho GTPase activation; Mary Stofega, Celine DerMardirossian and Gary M. Bokoch; 17 Assay for phospholipase D in cell-free systems; Shanker S. Iyer and David Kusner; 18 Reconstitution system based on cytosol-depleted cells to study regulation of phospholipase D; Amanda Fensome-Green and Shamshad Cockcroft; E. GENOMICS AND PROTEOMICS; 19 Analysis of Global Gene Expression Profiles Activated by Chemoattractant Receptors; Fernando O. Martinez, and Massimo Locati; 20 Genetic reconstitution of bone marrow for the study of signal transduction Ex Vivo.


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Product Details
  • ISBN-13: 9781597450485
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Language: English
  • ISBN-10: 1597450480
  • Publisher Date: 01 Jan 2006
  • Binding: Digital (delivered electronically)
  • Series Title: No. 332 Methods in Molecular Biology


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