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The Proteomics Protocols Handbook

The Proteomics Protocols Handbook


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About the Book

This important handbook provides a number of novel mass spectrometry and LC-MS techniques central to structural and functional proteomics that are needed to deduce the function of newly discovered protein sequences. The use of these techniques will lead to achieving the ultimate goal of proteomics, namely to catalog the identity and funtion of all proteins in living organisms.

Table of Contents:
Extraction and Solubilization of Proteins for Proteomic Studies; Richard M. Leimgruber; Preparation of Bacterial Samples for 2-D Page; Brian Berg Vandahl, Gunna Christiansen and Svend Birkelund; Preparation of Yeast Samples for 2-D Page; Joakim Norbeck; Preparation of Mammalian Tissue Samples for Two-Dimensional Electrophoresis; Frank Witzmann; Differential Detergent Fractionation of Eukaryotic Cells; Melinda L. Ramsby and Gregory S. Makowski; Serum or Plasma Sample Preparation for Two-Dimensional Gel Electrophoresis; Anthony G. Sullivan, Stephen Russell, Henry Brzeski, Richard I. Somiari and Craig D. Shriver; Preparation of Plant Protein Samples for 2-D Page; David W.M. Leung; Laser Assisted Microdissection for Sample Preparation; Darrell L. Ellsworth, Stephen Russell, Brenda Deyarmin, Anthony Sullivan, Henry Brzeski, Richard I. Somiari and Craig D. Shriver; Purification of Cellular and Organelle Populations by Fluorescence-Activated Cell Sorting for Proteome Analysis; William L. Godfrey, Colette J. Rudd, Sujata Iyer and Diether Recktenwald; Purification of Nucleoli From Lymphoma Cells and Solubilization of Nucleolar Proteins for 2-DE Separations; Regis Dieckmann, Yohanne Coute, Denis Hochstrasser, Jean-Jacques Diaz and Jean-Charles Sanchez; Pre-Fractionation of Complex Protein Mixtures for 2-D Page Using Reversed Phase Liquid Chromatography; Volker Badock and Albrecht Otto; Fractionation of Complex Proteomes by Microscale Solution Isoelectrofocusing Using Zoom IEF Fractionators to Improve Protein Profiling; Xun Zuo, Ki-Beom Lee and David W. Speicher; Large-Format Two-Dimensional Polyacrylamide Gel Electrophoresis; Henry Brzeski, Stephen Russell, Tony G. Sullivan, Richard Somiari & Craig Shriver; Analysis of Membrane Proteins by Two-Dimensional Gels; Michael Fountoulakis, 2-D Page of High Molecular Mass Proteins; Masamichi Oh-Ishi and Tadakazu Maeda; Using Ultra-Zoom Gels for High Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis; Sjouke Hoving, Hans Voshol and Jan van Oostrum; NEPHGE and pI Strip Proteomic Two-Dimensional Gel Electrophoretic Mapping of Lipid-Rich Membranes; Steven E. Pfeiffer, Yoshihide Yamaguchi, Cecilia B. Marta, Rashmi Bansal and Christopher M. Taylor; Silver Staining of 2-D gels; Julia Poland, Thierry Rabilloud and Pranav Sinha; Zn2 & Reverse Staining Technique; Carlos Fernandez-Patron; Multiplexed Proteomics Technology for the Fluorescence Detection of Glycoprotein Levels and Protein Expression Levels; Using Pro-Q Emerald and SYPRO Ruby dyes; Birte Schulenberg and Wayne F. Patton; Multiplexed Proteomics Technology for the Fluorescence Detection of Phosphorylation and Protein Expression Levels; Using Pro-Q Diamond and SYPRO Ruby Dyes; Birte Schulenberg, Terrie Goodman, Thomas Steinberg and Wayne F. Patton; Sensitive Quantitative Fluorescence Detection of Proteins in Gels Using SYPRO Ruby Protein Gel Stain; Birte Schulenberg, Nancy Ahnert and Wayne F. Patton; Rapid, Sensitive Detection of Proteins in Minigels with Fluorescent Dyes: Coomassie Fluor Orange, SYPRO Orange, SYPRO Red, and SYPRO Tangerine protein gel stains; Thomas H. Steinberg, Courtenay R. Hart and Wayne F. Patton; Difference Gel Electrophoresis in a High Throughput Environment; Richard I. Somiari, Stephen Russell, Stella B. Somiari, Anthony G. Sullivan, Darrell L. Ellsworth, Henry Brzeski and Craig D. Shriver; Statistical Analysis of 2-D Gel Patterns; Francoise Seillier-Moiseiwitsch; 2-DE Databases on the World Wide Web; Christine Hoogland, Khaled Mostaguir and Ron D. Appel; Computer Analysis of 2-D images; Patricia M. Palagi, Daniel Walther, Gerard Bouchet, Sonja Voordijk and Ron D. Appel; Comparing 2D Electrophoretic Gels Across Internet Databases: An Open Source Application; Peter F. Lemkin, Gregory C. Thornwall and Jai Evans; Sample Cleanup by Solid Phase Extraction/Pipette Tip Chromatography; Alastair Aitken; Protein Identification by In-Gel Digestion and Mass Spectrometric Analysis; Michele Learmonth and Alastair Aitken


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Product Details
  • ISBN-13: 9781592598908
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Language: English
  • ISBN-10: 1592598900
  • Publisher Date: 01 Jan 2005
  • Binding: Digital (delivered electronically)


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