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Home > Mathematics and Science Textbooks > Biology, life sciences > Cellular biology (cytology) > Cell Migration: Developmental Methods and Protocols(Methods in Molecular Biology)
Cell Migration: Developmental Methods and Protocols(Methods in Molecular Biology)

Cell Migration: Developmental Methods and Protocols(Methods in Molecular Biology)


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About the Book

Cell migration plays an essential role in a variety of biological processes including embryonic development, homeostasis, immune responses, wound healing and cancer metastasis. The power of genetics has allowed for the discovery of novel molecules and signaling pathways that are important to the regulation of cell migration. This book brings together a wide range of novel and state-of-the-art methodologies as well as various classic methods in migration research in cultured cells, different model organisms, and as applied to specialized cells in normal development and in disease. The first part provides and overview of the field and is followed by chapters that describe assays that apply to all cell migration studies in vitro. Part III includes cell migration assays for cancer cells, endothelial cells, and neurons both in vitro and in animal models. Part IV discusses cell migration assays in various model organisms while the last part combines chapters on biochemical methods to study the mechanisms of cell migration and several new approaches to its study.

Table of Contents:
Part I. Introduction; Cell Migration: An Overview; Donna J. Webb, Huaye Zhang, and Alan F. Horwitz; Part II. Basic Cell Migration and Related Assays; Boyden Chamber Assay; Hong-Chen Chen; Wound Healing Assay; Luis G. Rodriguez, Xiaoyang Wu, and Jun-Lin Guan; Analysis of Cell Migration Using the Dunn Chemotaxis Chamber and Time-Lapse Microscopy; Claire M. Wells and Anne J. Ridley; Cell Adhesion Assays; Dennis F. Kucik and Chuanyue Wu; Cell Spreading Assays; Allison L. Berrier and Susan E. LaFlamme; Cell Scatter Assay; Hong-Chen Chen; Cell Migration Analyses within Fibroblast-Derived 3-D Matrices; Edna Cukierman; Part III. Analysis of Cell Migration in Specialized Cell Systems; Tumor Cell Invasion Assays; Leslie M. Shaw; Analysis of Endothelial Cell Migration Under Flow; Song Li; Angiogenesis Assays in the Chick CAM; Chris Storgard, David Mikolon, and Dwayne G. Stupack; Investigations of Neuronal Migration in the Central Nervous System; Michael E. Ward and Yi Rao; Part IV. Analysis of Cell Migration in Model Organisms; Analysis of Cell Migration in C. elegans; M. Afaq Shakir and Erik A. Lundquist; Analysis of Cell Migration Using Drosophila as a Model System; Jocelyn A. McDonald and Denise J. Montell; Imaging Cell Movement During Dorsal Closure in Drosophila Embryos; William Wood and Antonio Jacinto; Analysis of Cell Movements in Zebrafish Embryos: Morphometrics and Measuring Movement of Labeled Cell; Populations In Vivo; Diane S. Sepich and Lilianna Solnica-Krezel; The Xenopus Embryo as a Model System for Studies of Cell Migration; Douglas W. DeSimone, Lance Davidson, Mungo Marsden, and Dominique Alfandari; Neural Crest Migration Methods in the Chicken Embryo; Maria Elena de Bellard and Marianne Bronner-Fraser; In Vivo and In Vitro Models of Mammalian Angiogenesis; Mien V. Hoang and Donald R. Senger; Part V. Biochemical and Novel Approaches in Cell Migration; Measurement of Protein Tyrosine Phosphorylations in Cell Adhesion; Vidhya V. Iyer, Christoph Ballestrem, Joachim Kirchner, Benjamin Geiger, and Michael D. Schaller; Cell Adhesion Assays: Fabrication of an E-cadherin Substratum and Isolation of Lateral and Basal Membrane Patches; Frauke Drees, Amy Reilein, and W. James Nelson; Application of microscope-based FRET to Study Molecular Interactions in Focal Adhesions of Live Cells; Christoph Ballestrem and Benjamin Geiger; Use of Multiphoton Imaging for Studying Cell Migration in the Mouse; Andrea Flesken-Nikitin, Rebecca M. Williams, Warren R. Zipfel, Watt W. Webb, and Alexander Yu; Using Microfluidic Channel Networks to Generate Gradients for Studying Cell Migration; Daniel S. Rhoads, Sharvari M. Nadkarni, Loling Song, Camilla Voeltz, Eberhard Bodenschatz, and Jun-Lin Guan


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Product Details
  • ISBN-13: 9781592598601
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Language: English
  • Sub Title: Developmental Methods and Protocols
  • ISBN-10: 1592598609
  • Publisher Date: 01 Jan 2005
  • Binding: Digital (delivered electronically)
  • Series Title: Methods in Molecular Biology


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