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Home > Mathematics and Science Textbooks > Chemistry > Analytical chemistry > Chromatography > Affinity Chromatography: Methods and Protocols(421 Methods in Molecular Biology)
Affinity Chromatography: Methods and Protocols(421 Methods in Molecular Biology)

Affinity Chromatography: Methods and Protocols(421 Methods in Molecular Biology)


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About the Book

Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era. It contains state-of-the-art, brand new protocols, and provides step-by-step laboratory instructions for readily reproducible results. Thirty-eight years after its introduction, affinity chromatography remains a key tool in the armory of separation techniques available to separation and interaction scientists.



Table of Contents:
Preface.- Contributors.- Affinity Chromatography: History, Perspectives, Limitations and ProspectsAna Ceclia A. Roque and Christopher R. Lowe 1.- Various Modes of Affinity Chromatography.- Immobilized Metal Ion Affinity Chromatography of Native ProteinsAdam Charlton and Michael Zachariou.- Affinity Precipitation of Proteins Using Metal ChelatesAshok Kumar, Igor Yu. Galaev, and Bo Mattiasson.- Immunoaffinity ChromatographyStuart R. Gallant, Vish Koppaka, and Nick Zecherle.- Dye Ligand ChromatographyStuart R. Gallant, Vish Koppaka, and Nick Zecherle.- Purification of Proteins Using Displacement ChromatographyNihal Tugcu.- Affinity Chromatography Using Purification Tags.- Rationally Designed Ligands for Use in Affinity Chromatography: An Artificial Protein LAna Ceclia A. Roque and Christopher R. Lowe.- Phage Display of Peptides in Ligand Selection for Use in Affinity ChromatographyJoanne L. Casey, Andrew M. Coley, and Michael Foley.- Preparation, Analysis and Use of an Affinity Adsorbent for the Purification of GST Fusion ProteinGareth M. Forde.- Immobilized Metal Ion Affinity Chromatography of Histidine-Tagged Fusion ProteinsAdam Charlton and Michael Zachariou.- Methods for the Purification of HQ-Tagged ProteinsBecky Godat, Laurie Engel, Natalie A. Betz, and Tonny M. Johnson.- Amylose Affinity Chromatography of Maltose-Binding Protein: Purification by both Native and Novel Matrix-Assisted Dialysis Refolding MethodsLeonard K. Pattenden and Walter G. Thomas.- Methods for Detection of Protein–Protein and Protein–DNA Interactions Using HaloTagMarjeta Urh, Danette Hartzell, Jacqui Mendez, Dieter H. Klaubert, and Keith Wood.- Site-Specific Cleavage of Fusion ProteinsAdam Charlton.- The Use of TAGZyme for the Efficient Removal of N-Terminal His-TagsJose? Arnau, ConniLauritzen, Gitte Ebert Petersen, and John Pedersen.- Various Applications of Affinity Chromatography.- Affinity Processing of Cell-Containing Feeds Using Monolithic Macroporous Hydrogels, CryogelsIgor Yu. Galaev and Bo Mattiasson.- Monolithic Bioreactors for MacromoleculesMojca Ben?ina, Katja Ben?ina, Aleš Podgornik, and Aleš Štrancar.- Plasmid DNA Purification Via the Use of a Dual Affinity ProteinGareth M. Forde.- Affinity Chromatography of Phosphorylated ProteinsGrigoriy S. Tchaga.- Protein Separation Using Immobilized Phospholipid ChromatographyTzong-Hsien Lee and Marie-Isabel Aguilar.- Analysis of Proteins in Solution Using Affinity Capillary ElectrophoresisNiels H. H. Heegaard, Christian Schou, and Jesper Østergaard.

Review :
From the reviews of the second edition: "The book consists of three parts including 21 chapters that have been written by a large group of authors from many countries. ... recommends this book as a manual for many specialists involved in purification of biomolecules in various fields of biochemistry, molecular biology, biotechnology, and bioorganic chemistry. The book can also be recommended as a handbook for university students and their teachers as well as for other schools preparing specialists in the above-mentioned fields." (G. Ya. Wiederschain, Biochemistry, November, 2008)


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Product Details
  • ISBN-13: 9781588296597
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Edition: Revised edition
  • Language: English
  • Returnable: N
  • Sub Title: Methods and Protocols
  • ISBN-10: 1588296598
  • Publisher Date: 30 Nov 2007
  • Binding: Hardback
  • Height: 229 mm
  • No of Pages: 348
  • Series Title: 421 Methods in Molecular Biology
  • Width: 152 mm


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