Stroke Genomics: Methods and Reviews(104 Methods in Molecular Medicine)

Leading experts explore the pragmatic application of the tools of genomic and molecular biology research to the treatment of stroke. Providing the reader with cutting-edge reviews of clinical and preclinical genomics, the authors relate changes ingene expression to physiological endpoints - such as functional imaging paradigms - to produce a more holistic approach in which molecular biology goes hand-in-hand with stroke pathophysiology. Topics of special interest include stem cell transplantation, gene therapy, clinical gene/gene interaction studies, and cytokine drug discovery. Readily reproducible techniques are provided, along with ancillary methods (imaging, animal models, human genetic study design, and transgenic animal production) that can be effectively applied to gene discovery. The book shows how to successfully design, execute, and interpret preclinical and clinical molecular biological studies of stroke, work that will greatly advance its understanding, as well as rapid development of the next generation of stroke therapeutics.

Table of Contents:
to Stroke Genomics.- Preclinical Models and Gene Manipulation.- Choice, Methodology, and Characterization of Focal Ischemic Stroke Models.- Mutant Animal Models of Stroke and Gene Expression.- Gene Therapy in Neurological Disease.- Stem Cell Transplantation After Middle Cerebral Artery Occlusion.- Endogenous Brain Protection.- Production of Transgenic and Mutant Mouse Models.- 3-Nitropropionic Acid Model of Metabolic Stress.- Clinical Paradigms.- Practicalities of Genetic Studies in Human Stroke.- Evaluation of the Interactions of Common Genetic Mutations in Stroke.- Assessing Differential Expression.- Technologies of Disease-Related Gene Discovery Using Preclinical Models of Stroke.- Quantitative Analysis of Gene Transcription in Stroke Models Using Real-Time RT-PCR.- Effective Analysis of Genomic Data.- Bioinformatic Approaches to Assigning Protein Function From Novel Sequence Data.- Pragmatic Target Discovery From Novel Gene to Functionally Defined Drug Target.

Review :
From the reviews: "The protocols presented in Stroke Genomics: Methods and Reviews will greatly benefit scientists interested in the molecular biology as well as the genetic and genomic basis of strokes." - Journal of Chemical Neuroanatomy "...very interesting and informative...an attractive and interesting overview of the different aspects of the current stroke research...can be recommended as a source of useful and up-to-date practical and theoretical information on stroke genetics and on stroke associated gene expression." - European Neurology "This book gives the opportunity for experts in the field to explore the practical and experimental applications of genomic and molecular biology research to the treatment of stroke. … The book also indicates detailed methods for studying the molecular biology of stroke. … It can be recommended if one is interested in the basic scientific background that underlies central nervous system pathology … ." (J. V. Byrne, Neuroradiology, Vol. 47, 2005)