Buy Phosphodiesterase Methods and Protocols - Bookswagon
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Home > Medicine & Health Science textbooks > Medical specialties, branches of medicine > Pharmacology > Phosphodiesterase Methods and Protocols: (307 Methods in Molecular Biology)
Phosphodiesterase Methods and Protocols: (307 Methods in Molecular Biology)

Phosphodiesterase Methods and Protocols: (307 Methods in Molecular Biology)


     0     
5
4
3
2
1



Available


X
About the Book

This volume provides a palette of a variety of conceptual and technical approaches, as well as tools in the PDE field allowing investigators to solve questions concerning the role of PDE isozyme and PDE isozyme tissue variants by addressing physiological functions or their implication in various pathologies. Phosphodiesterase Methods and Protocols will be invaluable to scientists, biochemists, molecular biologists, cell biologists and pharmacologists who wish to initiate or to deepen studies in the PDE field and to assist medicinal chemists and pharmaceutical reseachers in devising new approaches to drug design.

Table of Contents:
Study of Cyclic Adenosine Monophosphate Microdomains in Cells.- High-Resolution Measurements of Cyclic Adenosine Monophosphate Signals in 3D Microdomains.- Cygnets.- High-Throughput Screening of Phosphodiesterase Activity in Living Cells.- Assessment of Phosphodiesterase Isozyme Contribution in Cell and Tissue Extracts.- Localization of the Cyclic Guanosine 3?,5?-Monophosphate- Hydrolyzing Phosphodiesterase Type 9 in Rat Brain by Nonradioactive In Situ Hybridization.- Determination of Ca2+/Calmodulin-Stimulated Phosphodiesterase Activity in Intact Cells.- Adenovirus-Mediated Overexpression of Murine Cyclic Nucleotide Phosphodiesterase 3B.- Identification of Promoter Elements in the 5?-Flanking Region of Murine Cyclic Nucleotide Phosphodiesterase 3B Gene.- Purification of PDE6 Isozymes From Mammalian Retina.- Cyclic Guanosine 5?-Monophosphate Binding to Regulatory GAF Domains of Photoreceptor Phosphodiesterase.- Renaturation of the Catalytic Domain of PDE4A Expressed in Escherichia coli as Inclusion Bodies.- Determining the Subunit Structure of Phosphodiesterases Using Gel Filtration and Sucrose Density Gradient Centrifugation.- Crystallization of Cyclic Nucleotide Phosphodiesterases.- Generation of PDE4 Knockout Mice by Gene Targeting.- Immunoprecipitation of PDE2 Phosphorylated and Inactivated by an Associated Protein Kinase.- Investigation of Extracellular Signal-Regulated Kinase 2 Mitogen-Activated Protein Kinase Phosphorylation and Regulation of Activity of PDE4 Cyclic Adenosine Monophosphate-Specific Phosphodiesterases.- Radiolabeled Ligand Binding to the Catalytic or Allosteric Sites of PDE5 and PDE11.- Analysis of Dimerization Determinants of PDE6 Catalytic Subunits.- Interactions Between Catalytic and Inhibitory Subunits of PDE6.- Purification,Reconstitution on Lipid Vesicles, and Assays of PDE6 and Its Activator G Protein, Transducin.

Review :
"...interesting and thought provoking, but technically challenging...excellent guide to current research techniques in the field of phosphodiesterase." - Doody's Health Sciences Book Review Journal


Best Sellers


Product Details
  • ISBN-13: 9781588293145
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Height: 235 mm
  • No of Pages: 336
  • Series Title: 307 Methods in Molecular Biology
  • ISBN-10: 1588293149
  • Publisher Date: 22 Jun 2005
  • Binding: Hardback
  • Language: English
  • Returnable: Y
  • Width: 155 mm


Similar Products

Add Photo
Add Photo

Customer Reviews

REVIEWS      0     
Click Here To Be The First to Review this Product
Phosphodiesterase Methods and Protocols: (307 Methods in Molecular Biology)
Humana Press Inc. -
Phosphodiesterase Methods and Protocols: (307 Methods in Molecular Biology)
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

Phosphodiesterase Methods and Protocols: (307 Methods in Molecular Biology)

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Bookswagon (the "CRR Service").


    By submitting any content to Bookswagon, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Bookswagon (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Bookswagon a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Bookswagon may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Bookswagon's sole discretion. Bookswagon reserves the right to change, condense, withhold publication, remove or delete any content on Bookswagon's website that Bookswagon deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Bookswagon does not guarantee that you will have any recourse through Bookswagon to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Bookswagon reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Bookswagon, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Bookswagon, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept

    Fresh on the Shelf


    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!