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Home > Mathematics and Science Textbooks > Biology, life sciences > Life sciences: general issues > Evolution > Directed Evolution Library Creation: Methods and Protocols(231 Methods in Molecular Biology)
Directed Evolution Library Creation: Methods and Protocols(231 Methods in Molecular Biology)

Directed Evolution Library Creation: Methods and Protocols(231 Methods in Molecular Biology)


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About the Book

A comprehensive compendium of cutting-edge protocols for the generation of molecular diversity. Described in step-by-step detail to ensure experimental success, these protocols include readily reproducible methods for random mutagenesis of entire genes or segments of genes, for homologous and nonhomologus recombination, and for constructing in vivo libraries in bacteria and yeast. In addition to the various protocols for creating libraries, this volume also describes ways to analyze libraries, particularly those made by recombination. An accompanying volume, Directed Enzyme Evolution: Screening and Selection Methods (ISBN: 1-58829-286-X), is devoted entirely to selection and screening methods that can be applied to the directed evolution of enzymes. Copy for Both Volumes Directed Evolution Library Creation: Methods and Protocols and Directed Enzyme Evolution: Screening and Selection Methods constitute an extraordinary collection of all the key methods used today for directed evolution research. Described in step-by-step detail to ensure robust experimental results, these methods will enable both newcomers and more experienced investigators to design and implement directed evolution strategies for the engineering of novel proteins. The first volume describes methods for the creation of mutated DNA molecules, or DNA libraries, encoding variants of desired proteins. The second volume describes methods for screening DNA libraries to isolate mutant proteins that exhibit a specified function.

Table of Contents:
Mutagenesis and Recombination Methods.- Generating Mutant Libraries Using Error-Prone PCR.- Preparing Libraries in Escherichia coli.- Preparing Libraries in Saccharomyces cerevisiae.- Creating Random Mutagenesis Libraries by Megaprimer PCR of Whole Plasmid (MEGAWHOP).- Construction of Designed Protein Libraries Using Gene Assembly Mutagenesis.- Production of Randomly Mutated Plasmid Libraries Using Mutator Strains.- Evolution of Microorganisms Using Mutator Plasmids.- Random Insertion and Deletion Mutagenesis.- Random Oligonucleotide Mutagenesis.- Saturation Mutagenesis.- DNA Shuffling.- Family Shuffling with Single-Stranded DNA.- In Vitro DNA Recombination by Random Priming.- Staggered Extension Process (StEP) In Vitro Recombination.- RACHITT.- The Creation of ITCHY Hybrid Protein Libraries.- Preparation of SCRATCHY Hybrid Protein Libraries.- Sequence Homology-Independent Protein Recombination (SHIPREC).- Producing Chimeric Genes by CLERY.- Analysis of Library Diversity.- Analysis of Shuffled Libraries by Oligonucleotide Probe Hybridization.- Sequence Mapping of Combinatorial Libraries on Macro- or Microarrays.- Sequence Mapping of Combinatorial Libraries on Macro- and Microarrays.


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Product Details
  • ISBN-13: 9781588292858
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Height: 229 mm
  • No of Pages: 226
  • Series Title: 231 Methods in Molecular Biology
  • Width: 152 mm
  • ISBN-10: 1588292851
  • Publisher Date: 25 Apr 2003
  • Binding: Hardback
  • Language: English
  • Returnable: Y
  • Sub Title: Methods and Protocols


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