Laser Capture Microdissection: Methods and Protocols(v. 293 Methods in Molecular Biology)

Expert laboratorians noted for their optimized methods describe in step-by-step detail how to successfully carry out tissue-based laser microdissection in their own laboratory using the different systems available and to apply a wide range of sophisticated molecular technologies to the microdissected tissue samples. The highlights include gene expression profiling of primary tumor cell populations using laser capture microdissection, RNA transcript amplification and GeneChip microarrays, laser capture microdissection and colorectal cancer proteomics, and proteomic analysis of human bladder tissue using SELDI approach following microdissection techniques. Applications of the microdissected tissue described encompass PCR, gene expression studies including microarray analysis, and proteomics.

Table of Contents:
Part I. Introduction An Introduction to Laser-Based Tissue Microdissection Techniques Stephanie Curran and Graeme I. Murray Part II. Microdissection and DNA Analysis Methacarn Fixation for Genomic DNA Analysis in Microdissected Cells Makoto Shibutani and Chikako Uneyama Multiplex Quantitative Real-Time PCR of Laser Microdissected Tissue Patrick H. Rooney Comparative Genomic Hybridization Using DNA From Laser Capture Microdissected Tissue Grace Callagy, Lucy Jackson, and Carlos Caldas Detection of Ki-ras and p53 Mutations by Laser Capture Microdissection/PCR/SSCP Deborah Dillon, Karl Zheng, Brina Negin, and Jose Costa Whole-Genome Allelotyping Using Laser Microdissected Tissue Colleen M. Feltmate and Samuel C. Mok Microdissection for Detecting Genetic Aberrations in Early and Advanced Human Urinary Bladder Cancer Arndt Hartmann, Robert Stoehr, Peter J. Wild, Wolfgang Dietmaier, and Ruth Knuechel Laser Microdissection for Microsatellite Analysis in Colon and Breast Cancer Peter J. Wild, Robert Stoehr, Ruth Knuechel, Arndt Hartmann, and Wolfgang Dietmaier Assessment of RET/PTC Oncogene Activation in Thyroid Nodules Utilizing Laser Microdissection Followed by Nested RT-PCR Giovanni Tallini and Guilherme Brandao Combined Laser-Assisted Microdissection and Short Tandem Repeat Analysis for Detection of In Situ Microchimerism After Solid Organ Transplantation Ulrich Lehmann, Anne Versmold, and Hans Kreipe Part III. RNA and Gene Expression Studies Using Microdissected Cells, Laser-Assisted Microdissection of Membrane-Mounted Tissue Sections Lise Mette Gjerdrum and Stephen Hamilton-Dutoit. Laser-Assisted Microdissection of Membrane-Mounted Sections Following Immunohistochemistry and In Situ Hybridization Lise Mette Gjerdrum and Stephen Hamilton-Dutoit Laser-Assisted Cell Microdissection Using the PALM System Patrick Micke, Arne A-stman, Joakim Lundeberg, and Fredrik Ponten Laser Microdissection and RNA Analysis Ludger Fink and Rainer Maria Bohle Gene Expression Profiling of Primary Tumor Cell Populations Using Laser Capture Microdissection, RNA Transcript Amplification, and GeneChip(R) Microarrays Veronica I. Luzzi, Victoria Holtschlag, and Mark A. Watson Quantification of Gene Expression in Mouse and Human Renal Proximal Tubules Jun-ya Kaimori, Masaru Takenaka, and Kousaku Okubo Laser Capture Microdissection for Analysis of Macrophage Gene Expression From Atherosclerotic Lesions Eugene Trogan and Edward A. Fisher Analysis of Pituitary Cells by Laser Capture Microdissection Ricardo V. Lloyd, Xiang Qian, Long Jin, Katharina Ruebel, Jill Bayliss, Shuya Zhang, and Ikuo Kobayaski Part IV. Microdissection Techniques and Applications in Proteomics Laser Capture Microdissection and Colorectal Cancer Proteomics Laura C. Lawrie and Stephanie Curran Proteomic Analysis of Human Bladder Tissue Using SELDI(R) Approach Following Microdissection Techniques Rene C. Krieg, Nadine T. Gaisa, Cloud P. Paweletz, and Ruth Knuechel Part V. Microdissection and Molecular Analysis of Microorganisms Genetic Analysis of HIV by In Situ PCR-Directed Laser Capture Microscopy of Infected Cells Daniele Marras Use of Laser Capture Microdissection Together With In Situ Hybridization and Real-Time PCR to Study the Distribution of Latent Herpes Simplex Virus Genomes in Mouse Trigeminal Ganglion Xiao-Ping Chen, Marina Mata, and David J. Fink Laser Capture Microdissection and PCR for Analysis of Human Papilloma Virus Infection Kheng Chew, Patrick H. Rooney, Margaret E. Cruickshank, and Graeme I. Murray Laser Capture Microdissection of Hepatic Stages of the Human Parasite Plasmodium falciparum for Molecular Analysis Jean-Philippe Semblat, Olivier Silvie, Jean-Francois Franetich, and Dominique Mazier Index

Review :
From the reviews: "...an excellent help to researchers..." - Neoplasma "Comprehensive and state of the art, this book provides a highly practical guide to successful tissue-based laser microdissection using available commercial systems, and also a guide to interpreting the results using a wide range of sophisticated molecular technologies." - Clinical Laboratory International "...provides researchers with practical advise and detailed technical protocols for carrying out tissue-based LCM..." - Vet Pathol "Comprehensive and up-to-date collection of readily reproducible preparative and analytical methods is presented in each chapter for an in-depth practice of laser microdissection application. ! The book is subdivided into 5 parts with a total of 24 chapters featuring step-by-step protocols that will enable novice researchers to isolate specific cell types or microorganisms." (Carlo Alberto Redi, European Journal of Histochemistry, December, 2005)