Generation of cDNA Libraries
Home > Mathematics and Science Textbooks > Biology, life sciences > Life sciences: general issues > Genetics (non-medical) > Generation of cDNA Libraries: Methods and Protocols(221 Methods in Molecular Biology)
Generation of cDNA Libraries: Methods and Protocols(221 Methods in Molecular Biology)

Generation of cDNA Libraries: Methods and Protocols(221 Methods in Molecular Biology)


     0     
5
4
3
2
1



Available


X
About the Book

Since its invention and subsequent development nearly 20 years ago, po- merase chain reaction (PCR) has been extensively utilized to identify numerous gene probes in vitro and in vivo. However, attempts to generate complete and full-length complementary cDNA libraries were, for the most part, fruitless and remained elusive until the last decade, when simple and rapid methods were developed. With current decoding and potential application of human genome information to genechips, there are urgent needs for identification of functional significance of these decoded gene sequences. Inherent in bringing these app- cations to fruition is the need to generate a complete and full-length cDNA library for potential functional assays of specific gene sequences. Generation of cDNA Libraries: Methods and Protocols serves as a laboratory manual on the evolution of generation of cDNA libraries, covering both ba- ground information and step-by-step practical laboratory recipes for which p- tocols, reagents, operational tips, instrumentation, and other requirements are detailed. The first chapter of the book is an overview of the basics of generating cDNA libraries, which include the following: (a) the definition of a cDNA library, (b) different kinds of cDNA libraries, (c) differences between methods for cDNA library generation using conventional approaches and novel stra- gies, including reverse generation of RNA repertoires from cDNA libraries, and (d) the quality of cDNA libraries.

Table of Contents:
Complementary DNA Libraries.- Rapid Amplification of cDNA Ends.- cDNA Generation on Paramagnetic Beads.- Construction of a Normalized cDNA Library by mRN-cDNA Hybridization and Subtraction.- Amplification of cDNA Ends Using PCR Suppression Effect and Step-Out PCR.- Use of Inverse PCR to Clone cDNA Ends.- Construction of Size-Fractionated cDNA Library Assisted by an In Vitro Recombination Reaction.- Construction of a Full-Length Enriched and a 5?-End Enriched cDNA Library Using the Oligo-Capping Method.- cDNA Library Construction Using In Vitro Transcriptional Amplification.- Amplification of Representative cDNA Pools from Microscopic Amounts of Animal Tissue.- Single-Cell cDNA Library Construction Using Cycling aRNA Amplification.- mRNA/cDNA Library Construction Using RNA—Polymerase Cycling Reaction.- Quality Assessment of cDNA Libraries.- Assessment of the Quality of mRNA Libraries by Agarose Gel Electrophoresis.- PACS RT-PCR.- Single-Cell mRNA Library Analysis by Northern Blot Hybridization.- Generation of cDNA Libraries for Profiling Gene Expression of Given Tissues or Cells.- Screening Poly [dA/dT(?)] cDNA for Gene Identification.- Generation of Longer cDNA Fragments from SAGE Tags for Gene Identification.- Generation of Full-Length cDNA Libraries Enriched for Differentially Expressed Genes.- Subtractive Hybridization for the Identification of Differentially Expressed Genes Using Uraci-DNA Glycosylase and Mung-Bean Nuclease.- Subtractive Cloning of Differential Genes Using RNA-PCR.- Strategy for Construction of a cDNA Encoding a Repetitive Amino Acid Sequence.- Preparing Lambda Libraries for Expression of Proteins in Prokaryotes or Eukaryotes.- Peptide Library Construction from RNA-PCR-Derived RNAs.- Identifying Interacting Proteins in an Escherichiacoli-Based Two-Hybrid System.- Future Perspectives.

Review :
...the ready-for-use protocols are excellent...A good buy. - Cell Biochem Funct


Best Sellers


Product Details
  • ISBN-13: 9781588290663
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Height: 235 mm
  • No of Pages: 338
  • Series Title: 221 Methods in Molecular Biology
  • Width: 155 mm
  • ISBN-10: 1588290662
  • Publisher Date: 19 Feb 2003
  • Binding: Hardback
  • Language: English
  • Returnable: Y
  • Sub Title: Methods and Protocols


Similar Products

Add Photo
Add Photo

Customer Reviews

REVIEWS      0     
Click Here To Be The First to Review this Product
Generation of cDNA Libraries: Methods and Protocols(221 Methods in Molecular Biology)
Humana Press Inc. -
Generation of cDNA Libraries: Methods and Protocols(221 Methods in Molecular Biology)
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

Generation of cDNA Libraries: Methods and Protocols(221 Methods in Molecular Biology)

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Bookswagon (the "CRR Service").


    By submitting any content to Bookswagon, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Bookswagon (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Bookswagon a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Bookswagon may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Bookswagon's sole discretion. Bookswagon reserves the right to change, condense, withhold publication, remove or delete any content on Bookswagon's website that Bookswagon deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Bookswagon does not guarantee that you will have any recourse through Bookswagon to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Bookswagon reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Bookswagon, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Bookswagon, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept

    New Arrivals


    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!