Buy Herpes Simplex Virus Book by Cornel Fraefel - Bookswagon
close menu
Bookswagon
search
My Account
Home > Medicine & Health Science textbooks > Clinical and internal medicine > Diseases and disorders > Sexually transmitted infections / Venereal diseases > Herpes Simplex Virus: Methods and Protocols(2060 Methods in Molecular Biology)
Herpes Simplex Virus: Methods and Protocols(2060 Methods in Molecular Biology)

Herpes Simplex Virus: Methods and Protocols(2060 Methods in Molecular Biology)


     0     
5
4
3
2
1



Out of Stock


Notify me when this book is in stock
X
About the Book

This second edition volume expands on the previous edition with a discussion of new and updated methods used to study the Herpes Simplex Virus (HSV), along with a look at the latest developing technologies such as next generation sequencing, CRISPR/Cas9 engineering, and the use of BioID to identify protein-protein interactions. Chapters cover topics such as the biology, life cycle, and current state of antiviral and vaccine development for HSV-1; protocols on growing viruses in cell culture and manipulating viral DNA; design and application of HSV-1 vectors for cancer- and gene-therapy; and structural analyses, microscopy, proteomics, and testing of antivirals. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 Cutting-edge and comprehensive, Herpes Simplex Virus: Methods and Protocols, Second Edition is a valuable resource for immunologists, and molecular and cell biologists. This book will also be useful for researchers who wish to initiate molecular and/or cellular-based approaches to study HSV. 


Table of Contents:
Tour de Herpes: Cycling through the Life and Biology of HSV-1.- Vaccines for Herpes Simplex: Recent Progress Driven by Viral and Adjuvant Immunology.- Herpes Simplex Virus Growth, Preparation, and Assay.- Engineering HSV-1 Vectors for Gene Therapy.- Preparation of Herpes Simplex Virus Type 1 (HSV-1)-Based Amplicon Vectors.- HSV-1 Amplicon Vectors as Genetic Vaccines.- oHSV Genome Editing by Means of galK Recombineering.- Rescue, Purification, and Characterization of a Recombinant HSV Expressing a Transgenic Protein.- CRISPR/Cas9-Based Genome Editing of HSV.- Latent/Quiescent Herpes Simplex Virus 1 Genome Detection by Fluorescent In Situ Hybridization (FISH).- Oligonucleotide Enrichment of HSV-1 Genomic DNA from Clinical Specimens for use in High-Throughput Sequencing.- HSV Mutant Generation and Dual-Detection Methods for Gaining Insight into Latent/Lytic Cycles In Vivo.- Phenotypic and Genotypic Testing of HSV-1 and HSV-2Resistance to Antivirals.- Using Primary SCG Neuron Cultures to Study Molecular Determinants of HSV-1 Latency and Reactivation.- Characterization of Extracellular HSV-1 Virions by Proteomics.- Analysis and Sorting of Individual HSV-1 Particles by Flow Virometry.- Isolation/Analysis of Extracellular Microvesicles from HSV-1-Infected Cells.- Conformational Change in Herpes Simplex Virus Entry Glycoproteins Detected by Dot Blot.- BioID Combined with Mass Spectrometry to Study Herpesvirus Protein-Protein Interaction Networks.- Preparation of Herpes Simplex Virus Infected Primary Neurons for Transmission Electron Microscopy.- Transmission Immuno-Electron Microscopy of Herpes Simplex Virus-1 Infected Dorsal Root Ganglia Neurons Sectioned in Growth Plane.- Multi-Fluorescence Live Analysis of Herpes Simplex Virus Type-1 Replication.- Expression, Purification, and Crystallization of HSV-1 Glycoproteins for Structure Determination.- Expression, Purification, and Crystallization of Full-Length HSV-1 gB for Structure Determination.- The Use of Microfluidic Neuronal Devices to Study the Anterograde Axonal Transport of Herpes Simplex Virus-1.- A Model of In Vivo HSV-1 DNA Transport using Murine Retinal Ganglion Cells.- The Murine Intravaginal HSV-2 Challenge Model for Investigation of DNA Vaccines.

Review :

"The book is intended to help researchers in the field of herpes simplex virus research in understanding complex interactions between herpes virus and host. In my opinion, in addition to the researchers, the book will be quite useful for students and virologists who wish to further their knowledge in this field. The book does meet the needs of the intended audience by providing comprehensive, cutting-edge information on HSV methods and protocols." (Omer Iqbal, Doody's Book Reviews, October 2, 2020)


Best Sellers


Product Details
  • ISBN-13: 9781493998166
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Edition: Revised edition
  • Language: English
  • Returnable: N
  • Sub Title: Methods and Protocols
  • ISBN-10: 1493998161
  • Publisher Date: 16 Oct 2020
  • Binding: Paperback
  • Height: 254 mm
  • No of Pages: 457
  • Series Title: 2060 Methods in Molecular Biology
  • Width: 178 mm


Similar Products

Add Photo
Add Photo

Customer Reviews

REVIEWS      0     
Click Here To Be The First to Review this Product
Herpes Simplex Virus: Methods and Protocols(2060 Methods in Molecular Biology)
Humana Press Inc. -
Herpes Simplex Virus: Methods and Protocols(2060 Methods in Molecular Biology)
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

Herpes Simplex Virus: Methods and Protocols(2060 Methods in Molecular Biology)

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Bookswagon (the "CRR Service").


    By submitting any content to Bookswagon, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Bookswagon (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Bookswagon a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Bookswagon may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Bookswagon's sole discretion. Bookswagon reserves the right to change, condense, withhold publication, remove or delete any content on Bookswagon's website that Bookswagon deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Bookswagon does not guarantee that you will have any recourse through Bookswagon to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Bookswagon reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Bookswagon, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Bookswagon, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept

    Fresh on the Shelf


    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!
    Your IP: 216.73.216.124 IN