Therapeutic Applications of Ribozymes and Riboswitches: Methods and Protocols(1103 Methods in Molecular Biology)

In Therapeutic Applications of Ribozymes and Riboswitches: Methods and Protocols, expert researchers in the field provide a complete overview of protocols used in the development of RNA molecule as drugs and drug target. These include methods and protocols on recent and precise RNA molecule approaches or studies in the development of an RNA therapeutic tool. We are convinced that these methods will help researchers from various domains of life sciences, including clinicians, biochemists and virologists. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Therapeutic Applications of Ribozymes and Riboswitches: Methods and Protocols seeks to aid scientists in the further development of new therapeutic applications implicating RNA molecules or directly targeting harmful RNA.

Table of Contents:
Identification of Regulatory RNA in Bacterial Genomes by Genome-Scale Mapping of Transcription Start Sites.- Screening Inhibitory potential of anti-HIV RT RNA Aptamers.- Design and Evaluation of Clinically Relevant SOFA-HDV Ribozymes Targeting HIV RNA.- Directing RNase P-mediated Cleavage of Target mRNAs by Engineered External Guide Sequences in Cultured Cells.- Design and Analysis of Hammerhead Ribozyme Activity Against an Artificial Gene Target.- Knockdown Strategies for the Study of Proprotein Converstase and Proliferation in Prostate Cancer Cells.- Use of Tumor-Targeting Trans-Splicing Ribozyme for Cancer Treatment.- Characterization of Hairpin Ribozyme Reactions.- Finding Instances of Riboswitches and Ribozymes by Homology Search of Structured RNA with Infernal.- Structure-based Virtual Screening for the Identification of RNA-binding Ligands.- Probing Riboswitch Binding Sites with Molecular Docking, Focused Libraries, and in-line Probing Assays.- Discovery of Small Molecule Modifiers of microRNAs for the Treatment of HCV Infection.- Bacterial Flavin Mononucleotide Riboswitches as Targets for Flavin Analogs.- Construction and Application of Riboswitch-based Sensors That Detect Metabolites Within Bacterial Cells.- Screening Assays to Identify Artificial glmS Ribozyme Activators.- Analysis of Riboswitch Structure and Ligand Binding using Small-angle X-ray Scattering (SAXS).- Use of SHAPE to Select 2AP Substitution sites for RNA-ligand Interactions and Dynamics Studies.- Cell Internalization SELEX:  In Vitro Selection for Molecules that Internalize into Cells.- DNA Electronic Switches Based on Analyte-Responsive Aptamers.