Recombinant Gene Expression
Home > Medicine & Health Science textbooks > Pre-clinical medicine: basic sciences > Medical genetics > Recombinant Gene Expression: (824 Methods in Molecular Biology)
Recombinant Gene Expression: (824 Methods in Molecular Biology)

Recombinant Gene Expression: (824 Methods in Molecular Biology)


     0     
5
4
3
2
1



International Edition


X
About the Book

Studies related to recombinant gene expression have brought new advance such as  the emergence of the “omics” technologies. While Escherichia coli, Sacharomyces cerevisiae and insect cells continue to be the dominant production platforms of recombinant proteins. In Recombinant Gene Expression: Review and Protocols, Third Edition, expert researchers in the field detail many of the methods now commonly used to study recombinant gene expression. These include methods and techniques for bacteria, lower eukaryotes, fungi, plants and plant cells, and animals and animal cells. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Recombinant Gene Expression: Review and Protocols, Third Edition seeks to aid scientists in the further study of this crucially important research into recombinant gene expression.

Table of Contents:
Using Folding Promoting Agents in Recombinant Protein Production: A Review.-Routine Identity Confirmation of Recombinant Proteins by MALDI-TOF Mass Spectrometry.-A Matter of Packaging: Influence of Nucleosome Positioning on Heterologous Gene Expression.-Tools of the Trade: Developing Antibody-Based Detection Capabilities for Recombinant Proteins.-Heat-shock Protein Fusion Vectors for Improved Expression of Soluble Recombinant Proteins in Escherichia coli.-The use of a Flagellar Export Signal for the Secretion of Recombinant Proteins in Salmonella.-Optimization of Purification Protocols Based on the Step-by-step Monitoring of the Protein Aggregates in the Soluble Fractions.-Heterologous Protein Expression by Lactococcus lactis.-An Extended Suite of Genetic Tools for Use in Bacteria of the Halomonadaceae: An Overview.-Regulated Recombinant Protein Production in the Antarctic Bacterium Pseudoalteromonas haloplanktis TAC125.-A Novel Strategy for the Construction of Genomic Mutants of the Antarctic Bacterium Pseudoalteromonas haloplanktis TAC125.-A New Bacterial Co-expression System for Over-expressing Soluble Protein and Validating Protein-protein Interaction.-Heterologous High-level Gene Expression in the Photosynthetic Bacterium Rhodobacter capsulatus.-Plasmid DNA Production for Therapeutic Applications.-Recombinant Protein Production in the Eukaryotic Protozoan Parasite Leishmania tarentolae: A Review.-Expression of Multi-subunit Proteins in Leishmania tarentolae.-Recombinant Protein Production in Yeasts.-Yeasts as Tool for Heterologous Gene Expression.-The Cre/lox system: A Practical Tool to Efficiently Eliminate Selectable Markers in Fungal Endophytes.-Aptamer-regulated Expression of Essential Genes in Yeast.-Cloning and Expression of Hemicellulases from Aspergillus nidulans in Pichia pastoris.-A Thiamine-regulatable Epitope-tagged Protein Expression System in Fission.-Heterologous Gene Expression by Chromosomal Integration in Fission Yeast.-Genetic Engineering of Industrial Strains of Saccharomyces cerevisiae.-Recombinant Protein Production in Plants: Challenges and Solutions.-A Novel Plant Cell Bioproduction Platform for High-yield Secretion of Recombinant Proteins.-Super-promoter:TEV, a Powerful Gene Expression System for Tobacco Hairy Roots.-Bioseparation of Recombinant Proteins from Plant Extract with Hydrophobin Fusion Technology.-Quality Assessment of Recombinant Proteins Produced in Plants.-Cell-free Protein Synthesis as a Promising Expression System for Recombinant Proteins.-The use of Bacterial Artificial Chromosomes for Recombinant Protein Production in Mammalian Cell Lines.-Engineering the Chaperone Network of CHO Cells for Optimal Recombinant Protein Production and Authenticity.-High-throughput Baculovirus Expression in Insect Cells.-Recombinant Protein Expression in Milk of Livestock Species.            


Best Sellers


Product Details
  • ISBN-13: 9781493962211
  • Publisher: Humana Press Inc.
  • Binding: Paperback
  • Height: 254 mm
  • No of Pages: 649
  • Series Title: 824 Methods in Molecular Biology
  • ISBN-10: 1493962213
  • Publisher Date: 23 Aug 2016
  • Edition: Revised edition
  • Language: English
  • Returnable: Y
  • Width: 178 mm


Similar Products

Add Photo
Add Photo

Customer Reviews

REVIEWS      0     
Click Here To Be The First to Review this Product
Recombinant Gene Expression: (824 Methods in Molecular Biology)
Humana Press Inc. -
Recombinant Gene Expression: (824 Methods in Molecular Biology)
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

Recombinant Gene Expression: (824 Methods in Molecular Biology)

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Bookswagon (the "CRR Service").


    By submitting any content to Bookswagon, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Bookswagon (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Bookswagon a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Bookswagon may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Bookswagon's sole discretion. Bookswagon reserves the right to change, condense, withhold publication, remove or delete any content on Bookswagon's website that Bookswagon deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Bookswagon does not guarantee that you will have any recourse through Bookswagon to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Bookswagon reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Bookswagon, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Bookswagon, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept

    New Arrivals


    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!