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Home > Mathematics and Science Textbooks > Biology, life sciences > Biochemistry > Chiral Separations: Methods and Protocols(970 Methods in Molecular Biology)
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Chiral Separations: Methods and Protocols(970 Methods in Molecular Biology)

Chiral Separations: Methods and Protocols(970 Methods in Molecular Biology)


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About the Book

 There is a demand for analytical methods that are able to discriminate between enantiomers in order to analyze the enantiomeric purity of compounds from natural or chemical sources not only in pharmaceutical sciences but in any field on bioactive compounds including chemistry, biology, biochemistry, forensic, and environmental sciences and many others. The second edition of Chiral Separations: Methods and Protocols, expands upon the previous edition with current methodology, providing an overview and especially practically oriented applications of the most important analytical techniques in chiral separation sciences. New chapters on analytical separation sciences by chromatographic and electrophoretic techniques have been added as has simulated moving bed chromatography as a preparative method. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, Chiral Separations: Methods and Protocols, Second Edition is helpful for analytical chemists working on stereochemical problems in fields or pharmacy, chemistry, biochemistry, food chemistry, molecular biology, forensics, environmental sciences or cosmetics in academia, government or industry.

Table of Contents:
Chiral Recognition in Separation Science – An Overview.-Enantioseparations by Thin-Layer Chromatography.-Gas Chromatographic Enantioseparation of Unfunctionalized Chiral Hydrocarbons - An Overview.-HPLC-Enantioseparation on Cyclodextrin-based Chiral Stationary Phases.-Enantioseparations by High Performance Liquid Chromatography Using Polysaccharide-Based Chiral Stationary Phases – An Overview.-Common Screening Approaches for Efficient Analytical Method Development in LC and SFC on Columns Packed with Immobilized Polysaccharide-derived Chiral Stationary Phases.-Chiral Separations by HPLC on Immobilized Polysaccharide Chiral Stationary Phases.-Enantioseparations by High-Performance Liquid Chromatography Using Macrocyclic Glycopeptide-Based Chiral Stationary Phases – An Overview.-Enantioseparations of Primary Amino Compounds by High Performance Liquid Chromatography Using Chiral Crown Ether-Based Chiral Stationary Phase.-Screening of Pirkle-type Chiral Stationary Phases for HPLC Enantioseparations.-Enantioseparations by High Performance Liquid Chromatography Based on Chiral Ligand Exchange.-Enantioseparations by High Performance Liquid Chromatography Using Molecularly Imprinted Polymers.-Chiral Mobile Phase Additives in HPLC Enantioseparations.-Chiral Benzofurazan-Derived Derivatization Reagents for Indirect Enantioseparations by HPLC.-Separation of Racemic 1-(9-anthryl)-2,2,2-trifluoroethanol by Sub-/supercritical Fluid Chromatography.-Chiral Separations by Simulated Moving Bed Method Using Polysaccharide-Based Chiral Stationary Phases.-Enantioseparations by Capillary Electrophoresis Using Cyclodextrins as Chiral Selectors.-Application of Dual Cyclodextrin-Systems in Capillary Electrophoresis Enantioseparations.-Enantioseparations in Nonaqueous Capillary Electrophoresis Using Charged Cyclodextrins.-Use of Macrocyclic Antibiotics as the Chiral Selectors in Capillary Electrophoresis.-Application of Polymeric Surfactants in Chiral Micellar Electrokinetic Chromatography (CMEKC) and CMEKC Coupled to Mass Spectrometry.-Cyclodextrin-modified Micellar Electrokinetic Chromatography for Enantioseparations.-Cyclodextrin-mediated Enantioseparation in Microemulsion Electrokinetic Chromatography.-Chiral Separations by Capillary Electrophoresis Using Proteins as Chiral Selectors.-Enantioseparation by Chiral Ligand-Exchange Capillary Electrophoresis.-Experimental Design Methodologies in the Optimization of Chiral CE or CEC Separations – An Overview.-Chiral Capillary Electrophoresis-Mass Spectrometry.-Application of Chiral Ligand-Exchange Stationary Phases in Capillary Electrochromatography.-Polysaccharide-Derived Chiral Stationary Phases in Capillary Electrochromatography Enantioseparations.-Open Tubular Molecular Imprinted Phases in Chiral Capillary Electrochromatography.-Enantioseparations in Capillary Electrochromatography Using Sulfated Poly b-Cyclodextrin Modified Silica-Based Monolith as Stationary Phase.-Cyclodextrin-Mediated Enantioseparations by Capillary Electrochromatography.

Review :
From the reviews of the second edition: “The second edition of Chiral Separations: Methods and Protocols discusses almost every kind of chiral separation method, with the emphasis on fundamental research and experimental design. … it is a very useful book for scientists and students involved in the enantioseparation field, and scientists involved in R & D areas such as drug development and environmental monitoring can also benefit from this monograph. It is expected that this book will definitely promote research in chirality-related fields.” (Huwei Liu, Analytical and Bioanalytical Chemistry, Vol. 406, 2014) “The book provides an overview and practically oriented applications of the most important analytical techniques in chiral separations. … the chapters include introductions, lists of necessary materials and reagents, step-by-step laboratory protocols, and troubleshooting tips. Pharmacists, biochemists, chemists, food chemists, molecular biologists, and forensic and environmental scientists will find this book extremely useful.” (Omer Iqbal, Doody’s Book Reviews, August, 2013)


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Product Details
  • ISBN-13: 9781493959747
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Edition: Revised edition
  • Language: English
  • Returnable: Y
  • Sub Title: Methods and Protocols
  • ISBN-10: 1493959743
  • Publisher Date: 23 Aug 2016
  • Binding: Paperback
  • Height: 254 mm
  • No of Pages: 531
  • Series Title: 970 Methods in Molecular Biology
  • Width: 178 mm


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