Proteomic Profiling
Home > Mathematics and Science Textbooks > Biology, life sciences > Biochemistry > Proteomic Profiling: Methods and Protocols(1295 Methods in Molecular Biology)
Proteomic Profiling: Methods and Protocols(1295 Methods in Molecular Biology)

Proteomic Profiling: Methods and Protocols(1295 Methods in Molecular Biology)


     0     
5
4
3
2
1



International Edition


X
About the Book

This volume presents the latest developments of the main pillars of protein analysis, such as sample preparation, separation and characterization. The book begins by describing basic but important sample preparation protocols. It then goes on to describe more sophisticated procedures on enriching specific protein classes and concludes with detailed descriptions of integrated work-flows for comprehensive protein analysis and characterization. The authors of the individual chapters are renowned protein biochemists who have all set value to provide a detailed representation of their lab work. Throughout the chapters, these authors share important tips and tricks for a successful and reproducible employment of their protocols in other laboratories. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Proteomic Profiling: Methods and Protocols is the perfect guide for students of Biochemistry, Biomedicine, Biology, and Genomics and will be an invaluable source for the experienced, practicing scientists.

Table of Contents:
Mechanical/Physical Methods of Cell Disruption and Tissue Homogenization.- Sample Preservation Through Heat Stabilization of Proteins: Principles and Examples.- Isolating Peripheral Lymphocytes by Density Gradient Centrifugation and Magnetic Cell Sorting.- Investigating the Adipose Tissue Secretome: A Protocol to Generate High Quality Samples Appropriate for Comprehensive Proteomic Profiling.- Methods for Proteomics-Based Analysis of the Human Muscle Secretome Using an in vitro Exercise Model.- Urinary Pellet Sample Preparation for Shotgun Proteomic Analysis of Microbial Infection and Host Pathogen Interactions.- A Protocol for the Parallel Isolation of Intact Mitochondria from Rat Liver, Kidney, Heart and Brain.- Isolation of Mitochondria from Cultured Cells and Liver Tissue Biopsies for Molecular and Biochemical Analyses.- Dynamic Range Compression with ProteoMiner: Principles and Examples.- Qualitative and Quantitative Proteomic Analysis of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue.- Full-Length Protein Extraction Protocols and Gel-Based Downstream Applications in Formalin-Fixed Tissue Proteomics.- Enrichment of Low-Abundant Protein Targets by Immunoprecipitation Upstream of Mass Spectrometry.- P Isolation of Extracellular Vesicles for Proteomic Profiling.- A Protocol for Exosome Isolation and Characterization: Evaluation of Ultracentrifugation, Density-Gradient Separation, and Immunoaffinity Capture Methods.- Chloroplast Isolation and Affinity Chromatography for Enrichment of Low Abundant Proteins in Complex Proteomes.- Depletion of RuBisCO Protein Using the Protamine Sulfate Precipitation Method.- Step by Step Preparation of Proteins for Mass Spectrometric Analysis.- Identification of Protein N-Termini Using TMPP or Dimethyl Labeling and Mass Spectrometry.- Optimization of Cell Lysis and Protein Digestion Protocols for Protein Analysis by LC-MS/MS.- Comprehensive Protocol to Simultaneously Study Protein Phosphorylation, Acetylation and N-Linked Sialylated Glycosylation.- Protein Profiling and Phosphoprotein Analysis by Isoelectric Focusing.- Principles and Examples of Gel-Based Approaches for Phosphoprotein Analysis.- Neutral Phosphate-Affinity SDS-PAGE System for Profiling of Protein Phosphorylation.- Enrichment and Identification of Bacterial Glycopeptides by Mass Spectrometry.- In-Gel Peptide IEF Sample Preparation for LC/MS Analysis.- Western Blotting Using In-Gel Protein Labeling as a Normalization Control: Stain-Free Technology.- 2-D Western Blotting for Evaluation of Antibodies Developed for Detection of Host Cell Protein.- Free Flow Electrophoresis for Separation of Native Membrane Protein Complexes.- Three-Dimensional Electrophoresis for Quantitative Profiling of Complex Proteomes.- A Bead-Based Multiplex Sandwich Immunoassay to Assess the Abundance and Posttranslational Modification State of β-Catenin.- Identification of SUMO E3 Ligase Specific Substrates Using the HuProt Human Proteome Microarray.- Amyloid-Binding Proteins: Affinity Based Separation, Proteomic Identification and Optical Biosensor Validation.- Proteomic Profiling by Nanomaterials-Based Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry for High-Resolution Data and Novel Protein Information Directly from Biological Samples.

About the Author :
Bio-Rad Laboratories GmbH, 80939 München, Heidemannstr. 164 Sitz der Gesellschaft: München - Amtsgericht München HRB 7491


Best Sellers


Product Details
  • ISBN-13: 9781493925490
  • Publisher: Humana Press Inc.
  • Binding: Hardback
  • Language: English
  • Returnable: Y
  • Sub Title: Methods and Protocols
  • ISBN-10: 1493925490
  • Publisher Date: 28 Mar 2015
  • Height: 254 mm
  • No of Pages: 501
  • Series Title: 1295 Methods in Molecular Biology
  • Width: 178 mm


Similar Products

Add Photo
Add Photo

Customer Reviews

REVIEWS      0     
Click Here To Be The First to Review this Product
Proteomic Profiling: Methods and Protocols(1295 Methods in Molecular Biology)
Humana Press Inc. -
Proteomic Profiling: Methods and Protocols(1295 Methods in Molecular Biology)
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

Proteomic Profiling: Methods and Protocols(1295 Methods in Molecular Biology)

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Bookswagon (the "CRR Service").


    By submitting any content to Bookswagon, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Bookswagon (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Bookswagon a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Bookswagon may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Bookswagon's sole discretion. Bookswagon reserves the right to change, condense, withhold publication, remove or delete any content on Bookswagon's website that Bookswagon deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Bookswagon does not guarantee that you will have any recourse through Bookswagon to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Bookswagon reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Bookswagon, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Bookswagon, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept

    New Arrivals


    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!