Cell Analysis: Volume 1

The selective combination of physical, biochemical, and immunological prin- ciples, along with new knowledge concerning the biology of cells and advance- ments in engineering and computer sciences, has made possible the emergence of highly sophisticated and powerful methods for the analysis of cells and their constituents. This series on Cell Analysis is, therefore, aiming at providing the theoretical and practical background on how these methods work and what kind of information can be obtained. Cell Analysis will cover techniques on cell separation, cell identification and classification, characterization of orga- nized cellular components, functional properties of cells, and cell interactions. Applications in cell biology, immunology, genetics, toxicology, specific diseases, diagnostics and therapeutics, and other areas will be covered whenever relevant results exist. Nicholas Catsimpoolas Boston, Massachusetts vii Contents Chapter I Quantification of Red Blood Cell Morphology James W. Bacus I. History .. II. Details of Red Cell Measurements. 3 III. Cell Sample Population Distributions. 11 IV. Discussion and Summary. 25 References. 30 Chapter 2 Laser Microirradiation and Computer Video Optical Microscopy in Cell Analysis Michael W. Berns and Robert J. Walter I. Introduction 33 II. Laser Microbeams 34 III. Computer-Enhanced Video Microscopy for Laser Microsurgery.

Table of Contents:
1 Quantification of Red Blood Cell Morphology.- I. History.- II. Details of Red Cell Measurements.- III. Cell Sample Population Distributions.- IV. Discussion and Summary.- References.- 2 Laser Microirradiation and Computer Video Optical Microscopy in Cell Analysis.- I. Introduction.- II. Laser Microbeams.- III. Computer-Enhanced Video Microscopy for Laser Microsurgery.- IV. Chromosome Microsurgery.- V. Plant Cell Development (Chloroplast Irradiation).- VI. Developmental Cellular Neurobiology.- VII. Pattern Formation.- VIII. Conclusions.- References.- 3 Combination of Two Physical Parameters for the Identification and Separation of Lymphocyte Subsets.- I. Introduction.- II. Methods.- III. Applications.- IV. Conclusion.- References.- 4 Electrical Sizing of Cells in Suspension.- I. Introduction.- II. Theory.- III. Instrumentation.- IV. Operation.- V. Applications.- VI. The Future.- References.- 5 Light Scattering Analysis of Single Cells.- I. Introduction.- II. Theoretical Considerations.- III. Recent Applications of Forward and 90° Scatter.- IV. Multiangle and Multiwavelength Scattering.- V. Fourier Transform Techniques.- VI. Polarization Studies—New Directions.- VII. Conclusion.- References.- 6 Methods for Measuring Leukocyte Locomotion.- I. Introduction.- II. Visual Assays.- III. The Micropore Filter Assay.- IV. The Under-Agarose Assay.- V. Miscellaneous Techniques.- VI. Factors Influencing Locomotion.- References.- 7 Sizing of Cells by the Electrical Resistance Pulse Technique: Methodology and Application in Cytometric Systems.- I. Introduction.- II. Basic Events and Problems in Electrically Sizing Transducers.- III. Instrumentation.- IV. Calibration Problems of Electrical Sizing Flow Cytometers.- V. Applications of the Metricell Cell Volume Analyzer.- VI.Combination of Electrical Sizing with Multiparameter Optical Flow Analysis.- VII. Sorting Activated by Electrical Sizing.- VIII. Size-Triggered Imaging in Flow.- References.