A Laboratory Course in Tissue Engineering
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A Laboratory Course in Tissue Engineering

A Laboratory Course in Tissue Engineering


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About the Book

Filling the need for a lab textbook in this rapidly growing field, A Laboratory Course in Tissue Engineering helps students develop hands-on experience. The book contains fifteen standalone experiments based on both classic tissue-engineering approaches and recent advances in the field. Experiments encompass a set of widely applicable techniques: c

Table of Contents:
Getting Started in the Lab. Lab Safety. Essential Lab Skills for Tissue Engineers. Isolation of Primary Chondrocytes from Bovine Articular Cartilage. Measuring and Modeling Growth of a Cell Population. Purification of a Cell Population Using Magnetic Cell Sorting. Decellularized Matrices for Tissue Engineering. Effect of Plating Density on Cell Adhesion to Varied Culture Matrices. Dynamic versus Static Seeding of Cells onto Biomaterial Scaffolds. Cell Patterning Using Microcontact Printing. Measuring and Modeling the Motility of a Cell Population Using an Under-Agarose Assay. Characterizing Matrix Remodeling Through Collagen Gel Contraction. Effect of Substrate Stiffness on Cell Differentiation. Effect of Culture Configuration (Two versus Three Dimensions) on Matrix Accumulation. Combining In Silico and In Vitro Techniques to Engineer Pluripotent Stem Cell Fate. The Fahraeus-Lindqvist Effect: Using Microchannels to Observe Small Vessel Hemodynamics. Examining Single-Cell Mechanics Using a Microfluidic Micropipette Aspiration System. Contribution of Tissue Composition to Bone Material Properties. Technical Communication: Presenting Your Findings. Appendices. Index.

About the Author :
Melissa Kurtis Micou, Ph.D., is a lecturer in the Department of Bioengineering at the University of California, San Diego. She has taught tissue-engineering lecture and lab courses for undergraduate students for the past ten years. Dawn M. Kilkenny, Ph.D., is an assistant professor at the Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto, and is academic advisor to the IBBME undergraduate teaching laboratory. Her research interests include cellular signaling, fluorescent protein technology, and microscopy.

Review :
"The book is well organized to teach cell culture and tissue engineering experiments to novice and experienced students. There is a quantitative emphasis in the book that strengthens the 'engineering' part of tissue engineering." -Ann Saterbak, Rice University, Houston, Texas, USA "... an excellent handbook for graduate students and investigators who are new in tissue engineering area. ... The approaches and topics selected are appropriate for not only undergraduate but also graduate students and new investigators in this area." -Sha Jin, University of Arkansas, Fayetteville, USA "This book has a protocol-like style and can actually be used directly by teachers to prepare lab courses as well as by people with lab experience that enter the field of tissue engineering. ... provides students with good insight in methods, techniques and approaches in the field of tissue engineering." -Gerjo J.V.M. van Osch, Erasmus MC, University Medical Center Rotterdam, the Netherlands "... a much-needed book for undergraduate bioengineering curricula. Tissue engineering is a topic best learned through practice, and this book just might take the fear out of offering a laboratory course on the subject." -Michael J. Moore, Ph.D., Tulane University, New Orleans "... provides comprehensive coverage of laboratory techniques in tissue engineering, including detailed experimental protocols." -Adam Higgins, Oregon State University, Corvallis


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Product Details
  • ISBN-13: 9781466575233
  • Publisher: Taylor & Francis Inc
  • Publisher Imprint: CRC Press Inc
  • Language: English
  • ISBN-10: 1466575239
  • Publisher Date: 19 Apr 2016
  • Binding: Digital (delivered electronically)


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