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Journal of the New-York Microscopical Society (Volume 11-12)

Journal of the New-York Microscopical Society (Volume 11-12)


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Purchase of this book includes free trial access to www.million-books.com where you can read more than a million books for free. This is an OCR edition with typos. Excerpt from book: SOME REMARKS ON CLARIFICATION, AND ALSO ON A NEW CLARIFIER FOR MICRO- SCOPICAL PURPOSES. BY EDWIN A. SCHULTZE. (Read October igth, 1894.) One of the most important points to be observed in microscopical research is the requisite clarification of the object under examination. As simple, however, as this appears, the views of the different authorities who have investigated the subject do not appear to coincide. In studying plant sections clarification, according to Dippel, is resorted to to produce the necessary transparency of the object, which on account of its contents does not permit of a thorough investigation of the inner structure. The usual clarifiers mentioned by Dippel are: Liquor potassse (in some cases to be used with acetic acid and ammonia); potassic alcohol, carbolic acid (when necessary to be used after treatment with alcohol or mixed with turpentine), and finally chloral hydrate (8 parts dissolved in 5 of water). Zimmermann, in his work Botanische Mikro- technik, discriminates between the action of the above named chemical clarifiers and the clarification by means of more or less refractive mounting media, especially essential oils, balsams and resins. Of these latter he mentions Canada balsam, dammar and Venetian turpentine. Tschirch, in his Pflanzen anatomic, speaks of employing only liquor potassse and potassium chloride, with acetic acid for clarifying plant sections. Moeller, to conclude, very correctly places water at the head of his list of clarifying reagents; then follow glycerine, alkaline solutions, Javelle water, and finally chloral hydrate. There appears to be a difference of opinion among the authorities on this last mentioned clarifier, which Schimper frequently and highly recommends. Behrens, in his extensive catalogue of clarifiers, ...


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Product Details
  • ISBN-13: 9781459092198
  • Publisher: General Books
  • Publisher Imprint: General Books
  • Height: 246 mm
  • No of Pages: 66
  • Spine Width: 4 mm
  • Width: 189 mm
  • ISBN-10: 1459092198
  • Publisher Date: 01 Feb 2012
  • Binding: Paperback
  • Language: English
  • Returnable: N
  • Weight: 136 gr


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