Introduction to Elementary Practical Biology; A Laboratory Guide
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Introduction to Elementary Practical Biology; A Laboratory Guide

Introduction to Elementary Practical Biology; A Laboratory Guide


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Purchase of this book includes free trial access to www.million-books.com where you can read more than a million books for free. This is an OCR edition with typos. Excerpt from book: B.?Metazoa or Multicellular Animals. The following examples are cells from these animals, isolated from the body. Example 5.?Salivary Cells Material.?Rinse out the mouth two or three times with water, then collect some fresh saliva by spitting into a test-tube or a watch-glass. Let the glass stand quietly until the air-bubbles rise to the surface of the fluid and a sediment settles. The Avarm-stage, pipette, dilute iodine, magenta, one per cent. acetic acid, one per cent. acetic acid carmine, and indigo or carmine will be needed during the examination. Method of Examination.?With the pipette put a drop of the sediment on the slide and lay the cover-glass in position, with one edge resting on a piece of hair or a scrap of paper. Examine first with the low, then with the high power. In the preparation will usually be found many colorless flat cells, with irregularly polygonal outlines and large nuclei. These are epithelium cells from the lining membrane of the mouth, etc., and have nothing to do with the proper salivary cells. It will be well to examine their shape, size, structure, and the manner in which they are connected. Note also the very large nucleus, which may be made more distinct by staining with magenta or acetic acid carmine. MORPHOLOGY a. General Structure.?What is the shape of the cell? Size ? Color ? Does it have a cell-wall ? Nucleus? If so, what is its position ? Shape ? What doesthe cell contain ? Are the cells very abundant in the saliva ? Do they in their structure resemble any of the Protozoa ? Run a drop of magenta under the cover-glass, and note the changes in the cells. Prepare another specimen, and treat with dilute iodine. Test a third preparation with one per cent. acetic acid; a fourth with one per cent. acetic acid carmine. In each ...


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Product Details
  • ISBN-13: 9781459086869
  • Publisher: General Books
  • Publisher Imprint: General Books
  • Height: 246 mm
  • No of Pages: 104
  • Spine Width: 6 mm
  • Width: 189 mm
  • ISBN-10: 1459086864
  • Publisher Date: 12 Jan 2012
  • Binding: Paperback
  • Language: English
  • Returnable: N
  • Weight: 200 gr


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