Practical Manual of In Vitro Fertilization: Advanced Methods and Novel Devices

The Practical Manual of In Vitro Fertilization: Advanced Methods and Novel Devices is a unique, accessible title that provides a complete review of the most well-established and current diagnostic and treatment techniques comprising in vitro fertilization. Throughout the chapters, a uniform structure is employed, including a brief abstract, a keyword glossary, a step-by-step protocol of the laboratory procedures, several pages of expert commentary, key issues of clinical concern, and a list of references. The result is a readily accessible, high quality reference guide for reproductive endocrinologists, urologists, embryologists, biologists and research scientists. The Manual also offers an excellent description of novel procedures that will likely be employed in the near future. An indispensable resource for physicians and basic scientists, the Practical Manual of In Vitro Fertilization: Advanced Methods and Novel Devices is an invaluable reference and addition to the literature.

Table of Contents:
1. Journey of Human Gametes In Vitro: 1978 to 2010 Zeev Shoham I. Setting Up and Running an IVF Laboratory 2. Building the Laboratory Dean E. Morbeck and Marlena Duke 3. Air Quality Management Johan Guns, Ronny Janssens, and Martine Vercammen 4. Organizational Aspects of the Laboratory in a Tertiary Care ART Center Nancy L. Bossert and Christopher De Jonge 5. Quality Control Management William R. Boone and H. Lee Higdon III 6. Daily, Weekly, and Regular Preparations for the IVF Laboratory Michael A. Britt and Klaus E. Wiemer 7. Risk and Safety in the IVF Clinic Peter Sjoblom and Julius Hreinsson II. IVF Laboratory Equipment and Culture Systems 8. Essential Instruments and Disposable Supplies for an IVF Laboratory Leslie Weikert, Christa Fralick, and Klaus E. Wiemer 9. Co2 and Low-O2 Incubators Marius Meintjes 10. IVF Workstations Nicolas Prados and Alex C. Varghese 11. Culture Media in IVF: Decisions for the Laboratory Jason E. Swain and Thomas B. Pool III. In Vitro Fertilization 12. Oocyte Denuding Roberta Maggiulli, Filippo Ubaldi, and Laura Rienzi 13. Assessment of Oocyte Quality Basak Balaban, Turgay Barut, and Bülent Urman 14. Polarization Microscopy Markus Montag, Maria Köster, and Hans van der Ven 15. Cumulus Cell Gene Expression in Assessment of Oocyte Quality Dagan Wells IV. Embryo Culture Methods 16. Short Culture: Day 1/Day 2/Day 3 Embryo Culture Patrick Quinn 17. Extended Culture in IVF David K. Gardner and Michelle Lane 18. In Vitro Maturation of Human Oocytes Baris Ata, Jack Huang, and Ri-Cheng Chian 19. In Vivo Embryo Culture Device Claude Ranoux 20. Microfluidics for Gamete Manipulation and Embryo Culture Gary D. Smith, Charles Bormann, and Shuichi Takayama V. Sperm Processing and Selection 21. Sperm Assessment: Traditional Approaches and Their Indicative Value Margot Flint, Fanuel Lampiao, Ashok Agarwal, and Stefan S du Plessis 22. Sperm Assessment: Novel Approaches and Their Indicative Value De Y. Liu, Harold Bourne, C. Garrett, G. N. Clarke, Shlomi Barak, and H. W. G. Baker 23. Sperm Processing for IVF Ralf Henkel 24. PESA/TESA/TESE Sperm Processing Sandro C. Esteves and Sidney Verza, Jr. 25. Processing Sperm Samples in HIV-Positive Patients Thamara Viloria Samochin, Marcos Meseguer, Antonio Pellicer, José Remohí, and Nicolás Garrido 26. Intracytoplasmic Morphologically Selected Sperm Injection P. Vanderzwalmen, Magnus Bach, Batsuren Baramsai, A. Neyer, Delf Schwerda, Astrid Stecher, Barbara Wirleitner, Martin Zintz, Bernard Lejeune, S. Vanderzwalmen, Nino Guy Cassuto, Mathias Zech, and Nicolas H. Zech 27. Sperm Testing and ICSI Selection by Hyaluronic Acid Binding: The Hyaluronic Acid-Coated Glass Slide and Petri Dish in the Andrology and IVF Laboratories Gabor Huszar 28. Electrophoretic Sperm Separation Steven Fleming and John Aitken 29. Magnetic Activated Cell Sorting of Human Spermatozoa Enver Kerem Dirican 30. Poloscope-Based Sperm Selection Luca Gianaroli, M. Cristina Magli, Andor Crippa, Giorgio Cavallini, Eleonora Borghi, and Anna P. Ferraretti VI. Insemination Procedures 31. Intrauterine Insemination Gautam N. Allahbadia and Rubina Merchant 32. Conventional IVF Insemination Liesl Nel-Themaat, Thomas Elliott, Ching-Chien Chang, Graham Wright, and Zsolt Peter Nagy 33. Intracytoplasmic Sperm Injection Gianpiero D. Palermo, Queenie V. Neri, Devin Monahan, Takumi Takeuchi, Peter N. Schlegel, and Zev Rosenwaks 34. Mechanism of Human Oocyte Activation During ICSI and Methodology for Overcoming Low or Failed Fertilization Dmitri Dozortsev and Mohammad Hossein Nasr-Esfahani VII. Micromanipulators and Micromanipulation 35. Hydraulic Manipulators for ICSI Hubert Joris 36. Research Instruments Micromanipulators Steven Fleming and Catherine Pretty 37. Eppendorf Micromanipulator: Setup and Operation of Electronic Micromanipulators Ehab Abu-Marar and Safa Al-Hasani 38. The Leica-IMSI System Christiane Wittemer, Bruno Laborde, Frederic Ribay, and Stephane Viville 39. Automated Robotic Intracytoplasmic Sperm Injection Zhe Lu, Xinyu Liu, Xuping Zhang, Clement Leung, Navid Esfandiari, Robert F. Casper, and Yu Sun 40. Oocyte Treatment and Preparation for Microinjection Thomas Ebner 41. Livestock Production Via Micromanipulation Akira Onishi and Anthony C. F. Perry VIII. Embryo Evaluation, Grading, and Assisted Hatching 42. Pronuclear Scoring in Human In Vitro Fertilization Lynette Scott 43. Cumulative Morphological Assessment of Embryo Quality Barry Behr and Aparna Hegde 44. Metabolomics: The ViaMetrics-E™ Procedure for Assessing Embryo Viability D. Sakkas, L. Botros, M. Henson, K. Judge, and P. Roos 45. Oxygen Consumption as an Indicator of Oocyte and Embryo Viability Ana S. Lopes 46. Gene Expression Changes During Human Early Embryo Development: New Applications for Embryo Selection Samir Hamamah, Said Assou, Imène Boumela, Gisèle Ouandaogo, Hervé Dechaud, and John de Vos 47. Amino Acid Turnover as a Biomarker of Embryo Viability Roger Sturmey, Christine Leary, Henry Leese, and Danielle Smith 48. Real-time Embryo Monitoring Devices for Embryo Selection Gábor Vajta and Thorir Hardarson 49. Assisted Hatching in IVF Itziar Belil and Anna Veiga IX. Biopsy Procedures on Oocytes and Embryos 50. Polar Body Biopsy Markus Montag, Maria Köster, K. van der Ven, and Hans van der Ven 51. Cleavage Stage Embryo Biopsy Alan R. Thornhill 52. Embryo Biopsy for PGD: Current Perspective Steven J. McArthur, Don Leigh, Maria Traversa, James Marshall, and Robert P. S. Jansen 53. Microarrays and CGH for PGD of Chromosome Abnormalities and Gene Defects Gary Harton and Santiago Munné X. Cryopreservation 54. Sperm Cryopreservation Fabio Firmbach Pasqualotto, Eleonora Bedin Pasqualotto, Edson Borges, Jr., and Ashok Agarwal 55. Slow Freezing of Oocytes Giovanni Coticchio and Lucia De Santis 56. Vitrification: Research in Animal Models Gábor Vajta 57. Oocyte Vitrification Ana Cobo 58. Slow Freezing of Embryos Liesl Nel-Themaat, Ching-Chien Chang, Thomas Elliott, Patricia Bernal, Graham Wright, and Zsolt Peter Nagy 59. Vitrification of Embryos Juergen Liebermann, Joe Conaghan, Zsolt Peter Nagy, and Michael Tucker 60. Directional Freezing of Reproduction Cells, Tissues, and Organs Amir Arav 61. Ovarian Tissue Cryopreservation Jennifer L Kulp, J. Ryan Martin, and Pasquale Patrizio XI. Embryo Transfer 62. Assessment of Uterine Receptivity Hakan Cakmak and Hugh S. Taylor 63. Single Embryo Transfer Thorir Hardarson and Matts Wikland 64. Ultrasound-Guided Embryo Transfer (Abdominal/Vaginal): An Evidence-Based Evaluation Ahmed M. Abou-Setta 65. Cumulus-Aided Embryo Transfer Firuza R. Parikh, Nandkishor J. Naik, Dattatray J. Naik, and Dhanajaya Kulkarni XII. Management and Regulation in the ART Laboratory 66. Data Management in the ART Laboratory: Requirements and Solutions Timothy Brown and Bruce R. Gilbert 67. Regulation, Licensing, and Accreditation of the ART Laboratory Doris Baker 68. Legislation in the United Kingdom Rachel Cutting 69. Regulation, Licensing, and Accreditation of the ART Laboratory in Europe Julius Hreinsson and Peter Sjoblom 70. Regulation, Licensing, and Accreditation of ART Laboratories in India B. N. Chakravarty and Rita Modi 71. Troubleshooting in the Clinical Embryology Laboratory: The Art of Problem-Solving in ART Kathryn J. Go, Jay C. Patel, and Rick Dietz XIII. Special Topics 72. The Role of Mitochondria in the Establishment of Developmental Competence in Early Human Development Jonathan Van Blerkom 73. Nuclear and Cytoplasmic Transfer: Human Applications and Concerns Josef Fulka, Jr. and Helena Fulka 74. Cytoskeletal Architecture of Human Oocytes with Focus on Centrosomes and Their Significant Role in Fertilization Heide Schatten, Vanesa Y. Rawe, and Qing-Yuan Sun 75. Molecular Mining of Follicular Fluid for Reliable Biomarkers of Human Oocyte and Embryo Developmental Competence