Chromosome Nanoscience and Technology
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Chromosome Nanoscience and Technology

Chromosome Nanoscience and Technology


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About the Book

Despite progress in genetic research, knowledge about the exact structure of the chromosome continues to provide a challenge. Much of that challenge lies with the need for improved tools and methods that researchers require to perform novel analyses beyond the DNA level. Fortunately, rapid advances in nanotechnology, are now being employed to examine, analyze, and manipulate biological material at the chromosome level. Chromosome Nanoscience and Technology reviews these advances and their contribution to trends and applications in chromosome research. In addition to offering a guide to current progress, this book serves as the culminating report on a Japanese nanobiology project in the field of chromosome science begun in 2000. The project brought together researchers from disparate backgrounds that included molecular biology, biochemistry, protein science, immunology, genetics, anatomy, semiconductor production, polymer chemistry, material science, microscopy, and informatics, among others. Looking at chromosomes as nanomaterials, their contributions cover: Devices for chromosome handling, which includes the construction and application of nano and micro devices used for dissecting, analyzing, and manipulating chromosomes Visualization of chromosomes at nano and micro levels, which discusses methods for revealing nano-level folding of chromatin fibers Chromosomes as nanomaterials, which presents a new chromosome protein framework based on the cataloging of over 200 chromosomal proteins Informatics of chromosome images, which examines a new chromosome image database system for animals and plants This project, initiated a few years ago, now lays the groundwork for those scientists looking to perform further research in chromosome science. It provides them with starting points, as well as useful applications and methodology to assist in the long quest to gain a deepened understanding of life itself.

Table of Contents:
Preface. Mechanical Approaches to Elucidate Mechanisms of Chromosome Condensation at the Nano- and Micro-Level. Development of Novel AFM Probes for Chromosome Manipulation. Microchamber Array-Based Sequence-Specific DNA Detection from a Single Chromosome via Trace Volume PCR. On-Chip Chromosome Sorter Using Electric and Magnetic Fields. Fluorescence Microscopy for Analysis of Chromosome Dynamics. Fluorescence In Situ Hybridization (FISH) as a Tool for Comparative Genomics. Immunocytochemistry for Analyzing Chromosomes. Transmission and Scanning Electron Microscopy of Mammalian Metaphase Chromosomes. Atomic Force Microscopy of Human Chromosomes in Relation to Their Higher-Order Structure. Mechanism of Higher-Order Chromatin Folding Revealed by AFM Observation of In Vitro Reconstituted Chromatin. Scanning Near-Field Optical/Atomic Force Microscopy as a Tool for Simultaneous Specification of Chromosome Topography and Particular Gene Location on the Nanometer Scale. Isolation of Human and Plant Chromosomes as Nano-Materials. Proteome Analysis of Human Metaphase Chromosomes. Anti-Peptide Antibodies for Examining the Conformation and Molecular Assembly of an Intracellular Protein. Structure and Interactions of the Imitation SWI-Type Chromatin-Remodeling Complex, ATP-Dependent Chromatin-Assembly Factor. Dynamic and Functional Analysis of Chromosomal Proteins. Development of a Sustainable Chromosome Imaging Database. Image Database and Image Analysis of Chromosome Information.

About the Author :
Osaka University, Osaka, Japan Niigata University, Japan


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Product Details
  • ISBN-13: 9781420044911
  • Publisher: Taylor & Francis Inc
  • Publisher Imprint: CRC Press Inc
  • Height: 229 mm
  • No of Pages: 288
  • Width: 152 mm
  • ISBN-10: 1420044915
  • Publisher Date: 31 Oct 2007
  • Binding: Hardback
  • Language: English
  • Weight: 544 gr


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