Comparative Molecular Analysis of the Binding Between Severe Acute Respiratory Syndrome Coronavirus (Sars-Cov) Spike Protein and Angiotensin Converting Enzyme 2(ace2)
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Comparative Molecular Analysis of the Binding Between Severe Acute Respiratory Syndrome Coronavirus (Sars-Cov) Spike Protein and Angiotensin Converting Enzyme 2(ace2)

Comparative Molecular Analysis of the Binding Between Severe Acute Respiratory Syndrome Coronavirus (Sars-Cov) Spike Protein and Angiotensin Converting Enzyme 2(ace2)


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This dissertation, "Comparative Molecular Analysis of the Binding Between Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) Spike Protein and Angiotensin Converting Enzyme 2(ACE2)" by Chun-yip, Lam, 林俊業, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Abstract of thesis entitled COMPARATIVE MOLECULAR ANALYSIS OF THE BINDING BETWEEN SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS (SARS-CoV) SPIKE PROTEIN AND ANGIOTENSIN CONVERTING ENZYME 2 (ACE2) Submitted by Chun Yip LAM for the degree of Master of Philosophy at The University of Hong Kong in March 2007 The outbreak of SARS during 2002-2003 led to a tremendous impact on humans. The source of the pathogen was high concern. Isolation of the SARS associated coronavirus (SARS-CoV) from palm civet (Paguma larvata) and phylogenetic analysis suggested that palm civet is an intermediate host, which transmitted the disease to human. Recently, a number of diverse SARS-like CoVs were identified in horseshoe bats (Rhinolophus spp.). Horseshoe bats were recognized as a potential natural reservoir for SARS-CoV. On the other hand, angiotensin converting enzyme 2 (ACE2), the functional receptor of SARS-CoV, is highly conserved in animals. The aim of this study is to examine the possible host of SARS-CoV. This study focuses on the investigation of the molecular interaction between the spike protein of the SARS-CoV and ACE2 from 13 animal species. ACE2 from different animal species was cloned and sequenced. ACE2 tagged with green fluorescent protein (GFP) were expressed in Ad293 and HeLa cells. On the other hand, spike protein and red fluorescent protein (RFP) were co-expressed in Ad293 by the adenoviral expression system for a cell-base binding assay. SARS-CoV spike pseudovirus was also prepared for viral infection assay. With the aid of computational docking analysis, the data suggested that ACE2 of cat, dog and palm civet binds strongly to the spike protein and the level of binding is comparable with that of human ACE2. ACE2 of chinchilla, hamster, golden hamster, rabbit and rat binds to the spike protein moderately. Those of mouse, pig, horseshoe bat and Rickett's big footed bat show inferior capability in the binding of the spike protein of SARS-CoV. These results have several implications. Firstly, the inability of the spike protein to bind horseshoe bat's ACE2 and the large genetic distance between SARS-CoV and bat SARS-like CoV suggest the presence of another intermediate host. Secondly, the strong binding between the ACE2 of chinchilla and dog and spike protein of SARS-CoV suggest these animals may be highly susceptible to SARS-CoV. Surveillance on these animals is necessary to prevent the re-emergence of SARS. Finally, extra information on the susceptibility of the 13 kinds of animal may provide an insight on tracing the intermediate host of SARS-CoV. Continuous screening for the intermediate host is needed for a better understanding of the transmission path of SARS-like CoV from its natural reservoir to human. DOI: 10.5353/th_b3955701 Subjects: SARS (Disease) - Molecular aspects Coronaviruses Angiotensin converting enzyme Proteins - Analysis


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Product Details
  • ISBN-13: 9781374676756
  • Publisher: Open Dissertation Press
  • Publisher Imprint: Open Dissertation Press
  • Height: 279 mm
  • No of Pages: 134
  • Weight: 608 gr
  • ISBN-10: 1374676756
  • Publisher Date: 27 Jan 2017
  • Binding: Hardback
  • Language: English
  • Spine Width: 10 mm
  • Width: 216 mm

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Comparative Molecular Analysis of the Binding Between Severe Acute Respiratory Syndrome Coronavirus (Sars-Cov) Spike Protein and Angiotensin Converting Enzyme 2(ace2)
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