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Home > Mathematics and Science Textbooks > Biology, life sciences > Zoology and animal sciences > Molecular Epidemiology and Characterization of the Receptor Binding of Porcine Circovirus Type 2 (Pcv2)
Molecular Epidemiology and Characterization of the Receptor Binding of Porcine Circovirus Type 2 (Pcv2)

Molecular Epidemiology and Characterization of the Receptor Binding of Porcine Circovirus Type 2 (Pcv2)


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About the Book

This dissertation, "Molecular Epidemiology and Characterization of the Receptor Binding of Porcine Circovirus Type 2 (PCV2)" by Ching-man, Ma, 馬靜雯, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Abstract of thesis entitled MOLECULAR EPIDEMIOLOGY AND CHARACTERIZATION OF THE RECEPTOR BINDING OF PORCINE CIRCOVIRUS TYPE 2 (PCV2) Submitted by Ching Man MA for the degree of Master of Philosophy at The University of Hong Kong in November 2006 Porcine circovirus type 2 (PCV2) belongs to the family Circoviridae, which is the causative agent of postweaning multisystemic wasting syndrome (PMWS) in pigs. To investigate the evolution and pathogenicity of PCV2, molecular epidemiology was studied using a bioinformatic approach. In addition, receptor binding of PCV2 was studied using cell-binding assays. Classification of worldwide PCV2 strains designated five major PCV2 lineages (I-V) following phylogenetic analysis. It was found the local and worldwide strains were mainly originated from sub-lineage Ia. Evidences for recombination was shown in PCV2 strains from Hong Kong and mainland China. Molecular dating of PCV2 sub-lineage Ia using Bayesian Monte Carlo Markov chain (BMCMC) approach estimated that the emergence time of the most recent common ancestor (TMRCA) was around 1995. To study the receptor binding of PCV2, both cap genes (ORF2) of PCV1 and PCV2 without the nuclear localization signal (NLS) were expressed as glutathione S-transferase (GST)-fusion proteins in a bacterial expression system. The recombinant proteins GST-1.2-XNLS and GST-2.2-XNLS were demonstrated to bind to the porcine monocytic cell line 3D4/31 in a dose-dependent and specific manner. Both GST-1.2-XNLS and GST-2.2-XNLS showed reduced bindings in trypsin pre-treated cells and post-binding NaCl washing at different levels. Binding of GST-2.2-XNLS could not be significantly inhibited by GST-1.2-XNLS. Interestingly, both recombination proteins bound to all eight mammalian cell lines investigated, suggesting PCV1 and PCV2 use ubiquitous receptor(s) for cell attachment. However, different receptors might be responsible for successive infection. The current study provides information on evolution, receptor usage and pathogenicity of PCV2. DOI: 10.5353/th_b3822720 Subjects: DNA virus - Receptors Swine - Virus diseases - Genetic aspects Viral genetics Molecular epidemiology


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Product Details
  • ISBN-13: 9781374674813
  • Publisher: Open Dissertation Press
  • Publisher Imprint: Open Dissertation Press
  • Height: 279 mm
  • No of Pages: 136
  • Weight: 662 gr
  • ISBN-10: 1374674818
  • Publisher Date: 27 Jan 2017
  • Binding: Hardback
  • Language: English
  • Spine Width: 10 mm
  • Width: 216 mm


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Molecular Epidemiology and Characterization of the Receptor Binding of Porcine Circovirus Type 2 (Pcv2)
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