Buy Functional Ion Channels in Human Bone Marrow-Derived Mesenchymal Stem Cells and Human Cardiac C-Kit Progenitor Cells
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Home > Medicine & Health Science textbooks > Medicine: general issues > Functional Ion Channels in Human Bone Marrow-Derived Mesenchymal Stem Cells and Human Cardiac C-Kit Progenitor Cells
Functional Ion Channels in Human Bone Marrow-Derived Mesenchymal Stem Cells and Human Cardiac C-Kit Progenitor Cells

Functional Ion Channels in Human Bone Marrow-Derived Mesenchymal Stem Cells and Human Cardiac C-Kit Progenitor Cells


     0     
5
4
3
2
1



Out of Stock


Notify me when this book is in stock
X
About the Book

This dissertation, "Functional Ion Channels in Human Bone Marrow-derived Mesenchymal Stem Cells and Human Cardiac C-kit Progenitor Cells" by Yingying, Zhang, 张莹莹, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Ion channels play vital roles in maintaining physiological homeostasis and regulating a variety of physiological processes including cell proliferation and migration. The present PhD project investigated the potential roles of ion channels in regulating cell proliferation, differentiation, and/or migration in human bone marrow-derived mesenchymal stem cells (MSCs) and human cardiac c-kit+ progenitor cells using multiple approaches including electrophysiology, molecular biology, cell proliferation assays, flow cytometry, cell migration assays, etc. In first part of the study, roles of large-conductance Ca2+-activated potassium (BKCa or KCa1.1) channels and ether-a-go-go potassium (hEag1 or KCNH1) channels in regulating cell proliferation and adipogenesis and osteogenesis were determined in human MSCs. We found that inhibition of BKCa with paxilline or hEag1 with astemizole, or silencing BKCa with shRNAs targeting KCa1.1 or hEag1 channels with shRNAs targeting KCNH1 arrested the cells at G0/G1 phase via inhibiting signaling pathways including cyclin D1, cyclin E, p-ERK1/2, and p-Akt. Interestingly, silencing BKCa or hEag1 channels significantly reduced adipogenic differentiation and deceased osteogenic differentiation. These results demonstrate that BKCa and hEag1 channels not only regulate cell proliferation, but also participate in the adipogenic and osteogenic differentiations in human MSCs. In second part of the study, we found that several ionic currents were heterogeneously expressed in human cardiac c-kit+ progenitor cells, including BKCa in 86% of cells, an inwardly-rectifying K+ current (IKir) in 84% of cells, a transient outward K+ current (Ito) in 47% of cells, a voltage-gated tetrodotoxin-sensitive Na+ current (INa, TTX) in 61% of cells. Molecular identities of these ionic currents were determined with RT-PCR and Western blot analysis. KCa.1.1 (for BKCa), Kir2.1 (for IKir), Kv4.2 and Kv4.3 (for Ito), NaV1.2, NaV1.3, NaV1.6, NaV1.7 (for INa.TTX) were expressed in human cardiac progenitor cells. In third part of the study, we demonstrated that inhibition of BKCa with paxilline, Ito with 4-aminopyridine, but not INa.TTX with TTX or IKir with Ba2+, decreased cell proliferation in a concentration-dependent manner. Silencing KCa.1.1, or Kv4.2 and Kv4.3, but not Kir2.1 channels, reduced cell proliferation by accumulating the cells at G0/G1 phase. Blockade or knockdown of KCa.1.1, Kv4.2 or Kv4.3 channels, (but not INa.TTX)reduced cell migration. Interestingly, blockade of IKir or silencing Kir2.1 channels enhanced cell migration in human cardiac c-kit+ cells. Collectively, the present PhD project demonstrates the novel information that 1) BKCa and hEag1 regulate not only cell cycling, but also adipogenesis and osteogenesis in human MSCs; 2) multiple ion currents, BKCa, IKir, Ito, and INa, TTX, are present in human cardiac c-kit+ progenitor cells; and 3) BKCa and Ito, but not IKir and INa.TTX participate in regulating cell cycling. Interestingly, BKCa and Ito (but not INa.TTX) promote, while IKir inhibits cell proliferation in human cardiac c-kit+ progenitor cells DOI: 10.5353/th_b5060566 Subjects: Ion channels Stem cells Mesenchymal stem cells


Best Sellers


Product Details
  • ISBN-13: 9781361381939
  • Publisher: Open Dissertation Press
  • Publisher Imprint: Open Dissertation Press
  • Height: 279 mm
  • No of Pages: 228
  • Weight: 821 gr
  • ISBN-10: 1361381930
  • Publisher Date: 27 Jan 2017
  • Binding: Hardback
  • Language: English
  • Spine Width: 14 mm
  • Width: 216 mm


Similar Products

Add Photo
Add Photo

Customer Reviews

REVIEWS      0     
Click Here To Be The First to Review this Product
Functional Ion Channels in Human Bone Marrow-Derived Mesenchymal Stem Cells and Human Cardiac C-Kit Progenitor Cells
Open Dissertation Press -
Functional Ion Channels in Human Bone Marrow-Derived Mesenchymal Stem Cells and Human Cardiac C-Kit Progenitor Cells
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

Functional Ion Channels in Human Bone Marrow-Derived Mesenchymal Stem Cells and Human Cardiac C-Kit Progenitor Cells

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Bookswagon (the "CRR Service").


    By submitting any content to Bookswagon, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Bookswagon (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Bookswagon a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Bookswagon may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Bookswagon's sole discretion. Bookswagon reserves the right to change, condense, withhold publication, remove or delete any content on Bookswagon's website that Bookswagon deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Bookswagon does not guarantee that you will have any recourse through Bookswagon to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Bookswagon reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Bookswagon, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Bookswagon, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept

    Fresh on the Shelf


    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!